ELISA Assay Diluent (5X) 421203 from BioLegend

Product Specs
ItemELISA Assay Diluent (5X)
CompanyBioLegend
Price $71.00Supplier Page View Company Product Page
Catalog Number421203
Quantity100 mL
ApplicationsELISA

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BioLegend

8999 BioLegend Way

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Fax: 1-877-455-9587

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Website: biolegend.com

(1) Polymer-lipid hybrid nanoparticle enhances mRNA delivery and T cell-mediated immunitybioRxivJanuary 29, 2026Xiaolei Cai, Min Chen, Guoshuai Cao, Nicholas Asby, Derek Elli, Haley Gula, Vlad Nicolaescu, Duy-Thuc Nguyen, Xiaodan Huang, Tanushree Dangi, et al.200 uL 1×PBS+0.05% Tween 20 for three times and blocked by adding 200 μL 1×Assay Diluent (BioLegend #421203) into each well. The plates were washed gently with 200 uL 1×PBS+0.05% Tween 20 for three times after

(2) Highly Effective mRNA-LNP Vaccine Against Respiratory Syncytial Virus (RSV) in Multiple ModelsVaccines (Basel)June 10, 2025Huarong Bai, Xueliang Yu, Yue Gao, Qin Li, Baigang Wen, Rongkuan Huserially diluted with diluent. Then, 50 µL of the diluted sample or ELISA assay diluent (BIOLEGEND, 421203) was added to each well, and the plate was sealed and incubated at room temperature for 2 h. The liquid

(3) Validation and quantification of peptide antigens presented on MHCs using SureQuantNature ProtocolsMay 1, 2025Owen Leddy, Yufei Cui, Ryuhjin Ahn, Lauren Stopfer, Elizabeth Choe, Do Hun Kim, Malte Roerden, Stefani Spranger, Bryan D. Bryson, Forest M. White(https://www.biolegend.com/en-us/protocols/flex-t-hla-class-i-elisa-protocol). 5X ELISA Assay Diluent A (BioLegend Cat 421203, store at 4°C for up to 30 days after opening) 10X Dilution Buffer stock: 1 M NaCl, 0.5 M Tris, 1%

(4) Evaluation of safety, immunogenicity, and efficacy of inactivated reverse-genetics-based H5N8 highly pathogenic avian influenza virus vaccine with various adjuvants via parenteral and mucosal routes in chickensFrontiers in ImmunologyMarch 20, 2025Kairat Tabynov, Aidana Kuanyshbek, Leila Yelchibayeva, Kuantay Zharmambet, Zauresh Zhumadilova, Gleb Fomin, Nikolai Petrovsky, Olaitan C. Shekoni, Gourapura J. Renukaradhya, Kaissar Tabynovcoating buffer (#B288159, BioLegend). The coated plates were blocked using an ELISA Assay Diluent (#421203, BioLegend) at 200 μL per well and incubated under constant shaking (300–330 rpm on a PST-60HL thermal

(5) Detection of Amylin-β-amyloid Hetero-Oligomers by Enzyme-Linked Immunosorbent AssayBIO-PROTOCOLFebruary 5, 2025Noah S. Leibold, Deepak Kotiya, Nirmal Verma, Florin Despa(Anaspec, catalog number: AS-24235) Reagents 1. ELISA assay diluent (5×) (BioLegend, catalog number: 421203) 2. Dimethyl sulfoxide (DMSO) (Sigma, catalog number: D8418) 3. Na2CO3 (Sigma, catalog number: S7795) 4.

(6) An artificial transcription factor that activates potent interferon-γ expression in human Jurkat T CellsFront Mol MedJanuary 8, 2025Ashley King, Davis Noblitt, Olivia Sherron, Clara Kjerfve, Lydia Pless, Nicholas L. TruexPolysorbate-20 (3 × 100 µL) and blocked for 1 h with 200 µL of 1X the “Assay Diluent” buffer (BioLegend, 421203). Intracellular protein was isolated from the Jurkat cells after treatment with RIPA lysis buffer, quantified

(7) MCRS1 sensitizes T cell–dependent immunotherapy by augmenting MHC-I expression in solid tumorsJournal of Experimental MedicineDecember 2, 2024Xue Li, Han Yi, Zheyu Jin, Kaitao Jiang, Kangkang Xue, Jin Wang, Yuping Qian, Qian Xiang, Sijing Zhu, Runhe Yan, et al.experiments. The supernatant from Pmel-1 T cell co-culture was diluted by 500 folds with Assay Diluent Buffer (421203; BioLegend) and that from OT-I T cell co-culture was diluted by 1000 folds. 50 μl of standards or samples

(8) Prophylactic clemastine treatment improves influenza A virus-induced cognitive dysfunction in miceBrain, behavior, & immunity - healthDecember 1, 2024Tingling JD, Krauklis SA, Haak PL, Carr R, Louie AY, Johnson RW, Steelman AJ.(Sigma, P2287) at a volume of 200 μl per well. Plates were then blocked with ELISA diluent (BioLegend, 421203), 200 μl/well, for 1h at room temperature, then washed as described previously. Mouse serum samples

(9) Inhibition of ULK1/2 and KRASG12C controls tumor growth in preclinical models of lung cancereLifeAugust 30, 2024Phaedra C Ghazi, Kayla T O'Toole, Sanjana Srinivas Boggaram, Michael T Scherzer, Mark R Silvis, Yun Zhang, Madhumita Bogdan, Bryan D Smith, Guillermina Lozano, Daniel L Flynn, et al.(BioLegend, 421601), blocked for 1 hr at room temperature (RT) with 1× ELISA dilutant buffer (BioLegend Cat# 421203), then washed twice with 1× wash buffer. Cell extracts were diluted in wash buffer, incubated in the

(10) In vitro antibody-mediated SARS-CoV-2 infection suppression through human ACE2 receptor blockadeImmunology LettersAugust 1, 2024Priscilla S. Redd, Alyssa D. Merting, John D. Klement, Dakota B. Poschel, Dafeng Yang, Kebin Liuincubated at 4°C overnight. The wells were washed 3 times with PBS. 1x assay diluent (Biolegend, cat#421203, 200 μl/well) was added to the washed wells and incubated at room temperature for 1 h to block non-specific

(11) CAR NK92 Cells Targeting BCMA Can Effectively Kill Multiple Myeloma Cells Both In Vitro and In VivoBiomedicinesJanuary 22, 2024Park E, Mun HJ, Seo E, Hwang S, Lee JH, Song S, Sung H, Kim HY, Kwon MJ.harvested for ELISA. The concentration of IFN-γ in each well was determined using a human IFN-γ ELISA kit (#421203, Biolegend, San Diego, CA, USA). The concentration of granzyme B was determined using a human granzyme

(12) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.incubation at 4 °C, wells were washed with 0.05% Tween 20 in PBS three times and 200 μL blocking solution (421203, BioLegend) was added per well for one hour. Wells were then washed and incubated for three hours with

(13) Rapid, scalable assay of amylin-β amyloid co-aggregation in brain tissue and bloodThe Journal of biological chemistryMay 1, 2023Kotiya D, Leibold N, Verma N, Jicha GA, Goldstein LB, Despa F.of washing buffer [PBS with 0.05% of Tween 20 (0.05% PBST)] and blocked by 300 μl of assay diluent (421203, Biolegend) for 1 h at room temperature. Again, the plate was washed with 0.05% PBST washing buffer

(14) Human papillomavirus infection and seroprevalence among female university students in MexicoHuman Vaccines & ImmunotherapeuticsDecember 31, 2022Alexander Pedroza-Gonzalez et al.saline (PBS), pH 7.2 at 4°C overnight. The plates were blocked with ELISA Assay Diluent (Biolegend, Cat. 421203) at 4°C overnight. Plates were incubated with serum samples diluted 1:1000 in ELISA Assay Diluent for

(15) Development of an In-House EphA2 ELISA for Human Serum and Measurement of Circulating Levels of EphA2 in Hypertensive Patients with Renal DysfunctionDiagnostics (Basel)December 2, 2022Maki Murakoshi, Takumi Iwasawa, Takeo Koshida, Yusuke Suzuki, Tomohito Gohda, Kazunori Katoperoxidase (HRP, Cat. # 554066; Thermo Fisher Scientific, Waltham, MA, USA) in ELISA Assay Diluent (Cat. # 421203; BioLegend, San Diego, CA, USA) was added and maintained for 45 min at room temperature. Wells were

(16) Respiratory mucosal immunity against SARS-CoV-2 after mRNA vaccinationScience immunologyOctober 28, 2022Tang J, Zeng C, Cox TM, Li C, Son YM, Cheon IS, Wu Y, Behl S, Taylor JJ, Chakaraborty R, et al.washed with wash buffer (0.05% Tween 20 in PBS) and then blocked with Assay dilution buffer (Biolegend, 421203) for 1 hour at RT. Plasma or 20x concentrated BAL from unvaccinated donors, vaccinated and convalescents

(17) A Spike Protein-Based Subunit SARS-CoV-2 Vaccine for Pets: Safety, Immunogenicity, and Protective Efficacy in Juvenile CatsFrontiers in Veterinary ScienceMarch 14, 2022Kairat Tabynov, Madiana Orynbassar, Leila Yelchibayeva, Nurkeldi Turebekov, Toktassyn Yerubayev, Nurali Matikhan, Tlektes Yespolov, Nikolai Petrovsky, Kaissar TabynovCoating Buffer, #B288159, BioLegend) overnight. Plates were blocked using an ELISA Assay Diluent (#421203, BioLegend) at 200 μl per well and incubated under constant shaking (300–330 rpm on a PST-60HL thermal

(18) An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmissionNPJ vaccinesFebruary 23, 2022Tabynov K, Turebekov N, Babayeva M, Fomin G, Yerubayev T, Yespolov T, Li L, Renukaradhya GJ, Petrovsky N, Tabynov K.(ELISA Coating Buffer, #B288159, BioLegend) overnight. Plates were blocked using ELISA Assay Diluent (#421203, BioLegend) 200 µl/well and incubated under constant shaking (300-330 rpm on a PST-60HL thermal shaker,

(19) In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapyScience advancesJanuary 21, 2022Han JP, Kim M, Choi BS, Lee JH, Lee GS, Jeong M, Lee Y, Kim EA, Oh HK, Go N, et al.the plate was washed four times and then blocked by adding 100 μl of assay diluent buffer (BioLegend, 421203) for 1 hour at 25°C. Sera from mice injected with LNP-CRISPR-mAT or AAV9-Cas9 were diluted 40 times,

(20) Evaluating TNF-α and Interleukin-2 (IL-2) Levels in African American Primary Open-Angle Glaucoma PatientsGenesDecember 25, 2021Alapati T, Sagal KM, Gudiseva HV, Pistilli M, Pyfer M, Chavali VRM, O'Brien JM.similarly. To block non-specific binding and reduce background, 200 μL Assay Diluent (Biolegend Cat #421203, Biolegend, San Diego, CA, USA) with 1X PBS containing bovine serum was added to each well. The plate

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9/19/2019Increased Sensitivity of Homemade ELISA David TimmI'd give it a try with homemade ELISAs at least; it helped us.

ELISA Assay Diluent (5X) from BioLegend

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ELISA Assay Diluent (5X) from BioLegend

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