Increased Sensitivity of Homemade ELISA

Washington University
Psychiatry
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

ELISA assay diluent, ELISA diluent

Catalog Number:

421203

We were going by a protocol given to us. I tested with this diluent and it actually gave us better sensitivity. This could be important in the future for our experiments.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Cell supernatants

Tips

N/A

Results Summary

Better sensitivity

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Nearly everything

The Bad

Development time took longer than the homemade diluant.

The Bottom Line

I'd give it a try with homemade ELISAs at least; it helped us.

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