Free Glycerol Assay Kit ab65337 from Abcam

Product Specs
ItemFree Glycerol Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65337
Quantity100 Test
Sample TypeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay Time0h 40m
SensitivityMore than 1 µM
Research AreaMetabolism
Detection Range1 - 10000 µM
MethodFuncS

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Lymphocyte Antigen 6G Mediates Vagotomy-Associated Reduction in Body WeightFASEB JApril 15, 2026Ting Liu, April S. Caravaca, Min Cai, Wanmin Dai, Jérômine Jeanne Vacquié, Qi Guo, Peter Holicek, Maxime Villet, Patricia Teixeira Mendes, Yuyang Zhang, et al.#270–77 000) (n = 1 experiment). Glycerol was quantified using the Free Glycerol Assay Kit (Abcam, #ab65337) (n = 2 experiments). 2.6 Ex Vivo NEFA and CCL2 Release From eWAT eWAT was collected from male C57BL/6

(2) Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body WeightFASEB JApril 4, 2026Ting Liu, April S. Caravaca, Min Cai, Wanmin Dai, Jérômine Jeanne Vacquié, Qi Guo, Peter Holicek, Maxime Villet, Patricia Teixeira Mendes, Yuyang Zhang, et al.#270–77 000) (n = 1 experiment). Glycerol was quantified using the Free Glycerol Assay Kit (Abcam, #ab65337) (n = 2 experiments). 2.6 Ex Vivo NEFA and CCL2 Release From eWAT eWAT was collected from male C57BL/6

(3) Impaired Adipose Anabolism in Pancreatic Cancer Cachexia Is Reversed by HuR InhibitionJournal of Cachexia, Sarcopenia and MuscleMarch 19, 2026Paige C. Arneson‐Wissink, Katherine Pelz, Beth Worley, Heike Mendez, Peter Pham, Parham Diba, Peter R. Levasseur, Grace McCarthy, Alex Chitsazan, Jonathan R. Brody, et al.were run undiluted for glycerol quantification according to the manufacturer's instructions (Abcam ab65337). Fresh media and KPC CM alone (no 3T3‐L1 exposure) were below the detection limit of the assay. 2.4

(4) Co‐Treatment With rhBMP‐2 and Rapamycin Modulates Osteogenesis–Adipogenesis Balance to Enhance Aged Bone RegenerationAdvanced ScienceJanuary 28, 2026Zirui He, Xiaoqiao Bai, Fangru Xie, Xuan Wang, Fan Zhang, Yuanman Yu, Yuan Yuan, Changsheng Liufragments, the glycerol content in the supernatant was quantified using the Free Glycerol Assay Kit (Abcam, ab65337), according to the manufacturer's protocol. 5.9 Cell Culture in Conditioned Medium Senescent and young

(5) Portulaca oleracea-derived indoline amide ameliorates obesity and NAFLD in rats through Nrf2-dependent antioxidant and anti-inflammatory mechanismsSci RepOctober 8, 2025Mona Eid Alshamari, Laila Naif Al-Harbi, Ghedeir M. Alshammari, Maha H. Alhussain, Ali Saleh, Mohammed Abdo Yahyalipoprotein-cholesterol (LDL-c) and triglycerides (TG). Serum glycerol levels were quantified using the ab65337 kit from Abcam, Cambridge, UK. Additionally, alanine aminotransferase (ALT), gamma-glutamyl transpeptidase

(6) Lithocholic acid phenocopies anti-ageing effects of calorie restrictionNatureDecember 18, 2024Qi Qu, Yan Chen, Yu Wang, Shating Long, Weiche Wang, Heng-Ye Yang, Mengqi Li, Xiao Tian, Xiaoyan Wei, Yan-Hui Liu, et al.(isoallo-LCA; HY-B0172A) and 3-oxoallo-LCA (customized) were purchased from MCE. Free Glycerol Assay kit (ab65337) and Glycogen Assay kit (ab65620) were purchased from Abcam. Bacteriological peptone (LP0037) and yeast

(7) Royal Jelly Exerts a Potent Anti-Obesity Effect in Rats by Activating Lipolysis and Suppressing AdipogenesisNutrientsSeptember 19, 2024Alaa Hasanain Felemban, Ghedeir M. Alshammari, Abu ElGasim Ahmed Yagoub, Ali Saleh, Mohammed Abdo YahyaR&D System, Minneapolis, MN, USA). Free glycerol levels were assessed with a colorimetric assay kit (ab65337, Abcam, Cambridge, UK). Each assay was performed in duplicate, with n = 6 samples per group. 2.9. Cytokine

(8) Carthamus tinctorius L. (Safflower) Flower Extract Attenuates Hepatic Injury and Steatosis in a Rat Model of Type 2 Diabetes Mellitus via Nrf2-Dependent Hypoglycemic, Antioxidant, and Hypolipidemic EffectsAntioxidants (Basel)September 10, 2024Nuha Saad Alshareef, Sahar Abdulaziz AlSedairy, Laila Naif Al-Harbi, Ghedeir M. Alshammari, Mohammed Abdo YahyaFAs (FFAs) (Cat. No. MBS702165, Cat. No. MBS726298, and Cat. No. MBS014345); another kit (Cat. No. ab65337, Abcam, Cambridge, UK) was used to measure the serum levels of glycerol; the serum levels of alanine aminotransferase (ALT

(9) Light-responsive adipose-hypothalamus axis controls metabolic regulationNature communicationsAugust 8, 2024Tsuji T, Tolstikov V, Zhang Y, Huang TL, Camara H, Halpin M, Narain NR, Yau KW, Lynes MD, Kiebish MA, et al.were conducted following the manufacturer’s instructions using the free glycerol assay kit (Abcam, ab65337). Oil Red O staining Cells were washed twice with PBS and were subsequently fixed with 10% buffered

(10) Functional roles of pantothenic acid, riboflavin, thiamine, and choline in adipocyte browning in chemically induced human brown adipocytesScientific reportsAugust 6, 2024Takeda Y, Dai P.glycerol content, lactate secretion, and GPDH activity were measured using the Free Glycerol Assay Kit (ab65337, Abcam), Lactate Assay Kit-WST (L256, Dojindo), and GPDH Assay kit (AK01, Cosmo Bio Co., Ltd., Tokyo,

(11) Adaptive Metabolic Responses Facilitate Blood‐Brain Barrier Repair in Ischemic Stroke via BHB‐Mediated Epigenetic Modification of ZO‐1 ExpressionAdvanced ScienceJuly 1, 2024Ruijie Li, Yilin Liu, Jihao Wu, Xiong Chen, Qiying Lu, Kai Xia, Congyuan Liu, Xin Sui, Yixuan Liu, Yiling Wang, et al.and subsequently centrifuged at 3000 g for 10 min to collect serum. The concentration of glycerol (ab65337, Abcam), free fatty acids (MAK044, Sigma), triglycerides (MAK266, Sigma), low‐density lipoprotein (LDL,

(12) Angiotensin II participates in mitochondrial thermogenic functions via the activation of glycolysis in chemically induced human brown adipocytesScientific reportsMay 11, 2024Takeda Y, Yoshikawa T, Dai P.or Iso. The amounts of free glycerol and triglycerides were measured using Free Glycerol Assay Kit (ab65337, Abcam) and Triglyceride Assay kit (Ab65336, Abcam), respectively, according to the manufacturer’s instructions.

(13) Glycerol-weighted chemical exchange saturation transfer nanoprobes allow 19F/1H dual-modality magnetic resonance imaging-guided cancer radiotherapyNature CommunicationsOctober 20, 2023Rong A, Haoyu Wang, Chaoqun Nie, Zhaoguo Han, Meifang Zhou, Olagbaju Oluwatosin Atinuke, Kaiqi Wang, Xiance Wang, Shuang Liu, Jingshi Zhao, et al.free glycerol concentration in the dialysate samples, we applied a free glycerol assay kit (Abcam, ab65337). In the assay, glycerol is enzymatically oxidized to generate a product that reacts with the probe

(14) Breast cancer cell-derived exosome-delivered microRNA-155 targets UBQLN1 in adipocytes and facilitates cancer cachexia-related fat lossHuman molecular geneticsJune 19, 2023Sun S, Wang Z, Yao F, Sun K, Li Z, Sun S, Li C.Glycerol content in cell culture medium and serum was determined using the Free Glycerol Assay Kit (ab65337; Abcam) according to the manufacturer’s instructions. Reverse transcription–quantitative polymerase chain reaction

(15) Effect of Crocetin on Basal Lipolysis in 3T3-L1 AdipocytesAntioxidants (Basel)June 11, 2023Francisco J. Cimas, Miguel Ángel De la Cruz-Morcillo, Carmen Cifuentes, Natalia Moratalla-López, Gonzalo L. Alonso, Eduardo Nava, Sílvia Llorensmedium was collected to determine the extracellular glycerol content using a Free Glycerol Assay kit (ab65337, Abcam, Cambridge, United Kingdom) according to the protocol for the colorimetric assay provided by

(16) Luteolin-7-O-rutinoside Protects RIN-5F Cells from High-Glucose-Induced Toxicity, Improves Glucose Homeostasis in L6 Myotubes, and Prevents Onset of Type 2 DiabetesMetabolitesFebruary 14, 2023Pandurangan Subash-Babu, Sahar Abdulaziz AlSedairy, Manal Abdulaziz Binobead, Ali A. Alshatwiend of incubation, the medium was collected to measure the levels of lactate (ab282923), glycerol (ab65337) and free fatty acid (ab65341) concentrations using an enzymatic calorimetric kit purchased from Abcam

(17) Cancer cell-derived exosomal miR-425-3p induces white adipocyte atrophyAdipocyteDecember 1, 2022Liu A, Pan W, Zhuang S, Tang Y, Zhang H.Cell Proliferation Kit I (#11465007001, Sigma-Aldrich, St. Louis, MO, USA), Free Glycerol Assay Kit (ab65337, Abcam, Cambridge, MA, USA), cAMP Enzyme Immunoassay Kit (CA200, Sigma-Aldrich), and PKA Kinase Activity Assay Kit

(18) Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro StudiesCellsSeptember 16, 2022Lei Wang et al.the levels of free glycerol release and FFAs release from cells using the free glycerol assay kit (#ab65337, Abcam, The Netherlands) and FFAs assay kit (#ab65341, Abcam, The Netherlands). Cells lysates were used

(19) Capsaicin directly promotes adipocyte browning in the chemical compound-induced brown adipocytes converted from human dermal fibroblastsScientific reportsApril 22, 2022Takeda Y, Dai P.amount of free glycerol and non-esterified fatty acids (NEFAs) was measured by Free Glycerol Assay Kit (ab65337, Abcam) and Free Fatty Acid Assay Kit (ab65341, Abcam), respectively, according to the manufacturer’s

(20) Glycerol contained in vaping liquids affects the liver and aspects of energy homeostasis in a sex‐dependent mannerPhysiological ReportsJanuary 25, 2022Ariane Lechasseur et al.Carrez Clarification Reagent Kit (ab202373; Abcam). Free glycerol was measured according to the manufacturer's instructions (ab65337; Abcam). 2.4 Lung function measurement Mice were fasted at 20:00 on the day of the last exposure (5

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Free Glycerol Assay Kit from Abcam

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Free Glycerol Assay Kit from Abcam

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