Human TGF-beta 2 Quantikine ELISA Kit DB250 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman TGF-beta 2 Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberDB250
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range31.20 - 2000 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (125 uL), Serum (125 uL), EDTA Plasma (125 uL), Heparin Plasma (125 uL), Citrate Plasma (125 uL)
Sensitivity7 pg/mL
Detection TargetTGF-beta 2
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nametransforming growth factor beta 2
NCBI Gene AliasesG-TSF, LDS4, TGF-beta2

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Endothelial‐Mesenchymal Transition in Tumor Microenvironment Promotes Neuroendocrine Differentiation of Prostate CancerCancer ScienceOctober 1, 2025Takumi Kageyama, Manabu Kato, Shiori Miyachi, Xin Bao, Sho Sekito, Yusuke Sugino, Shinichiro Higashi, Takeshi Sasaki, Kouhei Nishikawa, Yasuhiro Murakawa, et al.ELISA Aliquots of conditioned medium were analyzed by ELISA (Quantikine kits: IL‐1β [DLB50], TGF‐β2 [DB250], GM‐CSF [DGM00]; Bio‐Techne, Minneapolis, MN, USA) to quantify protein levels per the manufacturer's

(2) TGFBR3 dependent mechanism of TGFB2 in smooth muscle cell differentiation and implications for TGFB2-related aortic aneurysmStem Cells Transl MedMarch 26, 2025Ying Tang, Jiaxi Cheng, Cynthia Huang, Ping Qiu, Jingxin Li, Yuqing Eugene Chen, Dogukan Mizrak, Bo Yangassessed in tissue ring lysates using a TGFB2 enzyme-linked immunosorbent assay (ELISA) kit (Catalog No. DB250, R&D Systems). Active TGFB2 levels were measured without sample acidification as described previously.40,41

(3) Involvement of USP7 in aggravating Kawasaki disease by promoting TGFβ2 signaling mediated endothelial-mesenchymal transition and coronary artery remodelingInternational immunopharmacologyJanuary 27, 2025Qian G, Wang Y, Yao H, Zhang Z, Wang W, Xu L, Li W, Huang L, Li X, Gao Y, et al.(IX81) confocal microscope. 2.10. Enzyme-linked immunosorbent assays (ELISAs) A human TGFβ2 ELISA Kit (DB250, R&D Systems, USA) and a human TNFα ELISA Kit (E-EL-H0109c, Elabscience, USA) were used in this study.

(4) Intratumor heterogeneity of EGFR expression mediates targeted therapy resistance and formation of drug tolerant microenvironmentNature communicationsJanuary 2, 2025Alsaed B, Lin L, Son J, Li J, Smolander J, Lopez T, Eser PÖ, Ogino A, Ambrogio C, Eum Y, et al.days of cell culture and TGFβ2 ELISA was conducted using manufacturer’s instructions (R&D Systems # DB250). Tumor organoid establishment and culture Primary lung tumor surgical samples were collected under

(5) The Expression of Serglycin Is Required for Active Transforming Growth Factor β Receptor I Tumorigenic Signaling in Glioblastoma Cells and Paracrine Activation of Stromal Fibroblasts via CXCR-2BiomoleculesApril 10, 2024Manou D, Golfinopoulou MA, Alharbi SND, Alghamdi HA, Alzahrani FM, Theocharis AD.Quantikine ELISA kit (DB100B, R&D Systems Inc., Minneapolis, MN, USA) and TGFβ2 Quantikine ELISA kit (DB250, R&D Systems Inc., Minneapolis, MN, USA), respectively, according to the manufacturer’s instructions. LN-18shSCR

(6) Aqueous Humor TGF-β2 and Its Association With Intraocular Pressure in a Naturally Occurring Large Animal Model of GlaucomaInvestigative ophthalmology & visual scienceJuly 3, 2023Oikawa K, Torne O, Sun D, Moon AKB, Kiland JA, Trane RM, McLellan GJ.samples were determined by a quantitative sandwich immunoassay (Quantikine ELISA assay kit, catalog # DB250; R&D Systems, Minneapolis, MN, USA) following the manufacturer's protocol as validated for feline samples

(7) Mesenchymal stromal cells promote the drug resistance of gastrointestinal stromal tumors by activating the PI3K-AKT pathway via TGF-β2Journal of translational medicineMarch 25, 2023Zhao Y, Weng Z, Zhou X, Xu Z, Cao B, Wang B, Li J.TGF- isoform. TGF-β1/2/3 was measured with a commercially available sandwich ELISA kit (Cat No. DB100C/DB250/DY243, R&D Systems, USA) according to the manufacturer's instructions. The mRNA expressions of TGF-β1,

(8) Impact of Sulfated Hyaluronan on Bone Metabolism in Diabetic Charcot Neuroarthropathy and Degenerative ArthritisInternational journal of molecular sciencesDecember 2, 2022Schulze S, Neuber C, Möller S, Hempel U, Hofbauer LC, Schaser KD, Pietzsch J, Rammelt S.

(9) How Effective Is a Late-Onset Antihypertensive Treatment? Studies with Captopril as Monotherapy and in Combination with Nifedipine in Old Spontaneously Hypertensive RatsBiomedicinesAugust 12, 2022Hawlitschek C, Brendel J, Gabriel P, Schierle K, Salameh A, Zimmer HG, Rassler B.

(10) Association between Maternal Characteristics and Immune Factors TGF-β1, TGF-β2, and IgA in Colostrum: An Exploratory Study in JapanNutrientsAugust 9, 2022Hirata N, Kiuchi M, Pak K, Fukuda R, Mochimaru N, Mitsui M, Yoshida K.TGF-β1 enzyme linked immunosorbent assay (ELISA) kit (DB100B) and Quantikine human TGF-β2 ELISA Kit (DB250) (both R&D Systems, Inc., Minneapolis, MN, USA) were used, respectively. TGF-β was activated prior to

(11) Maternal breast milk microbiota and immune markers in relation to subsequent development of celiac disease in offspringScientific reportsApril 22, 2022Štšepetova J, Simre K, Tagoma A, Uibo O, Peet A, Siljander H, Tillmann V, Knip M, Mändar R, Uibo R.

(12) Melanoma secretion of transforming growth factorâ€Î²2 leads to loss of epidermal AMBRA1 threatening epidermal integrity and facilitating tumour ulceration*British Journal of DermatologyApril 1, 2022I. Cosgarea, A.T. McConnell, T. Ewen, D. Tang, D.S. Hill, M. Anagnostou, M. Elias, R.A. Ellis, A. Murray, L.C. Spender, et al.control. TGF‐β2 secretion was measured using the TGF‐β2 Quantikine ELISA Kit (R & D Systems, Abingdon, UK; DB250) according to the manufacturer’s instructions. Small interfering RNA transfections Small interfering

(13) Oxidative Stress Enhances the TGF-β2-RhoA-MRTF-A/B Axis in Cells Entering Endothelial-Mesenchymal TransitionInternational journal of molecular sciencesFebruary 13, 2022Sobierajska K, Wawro ME, Niewiarowska J.

(14) TGF-β1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosisJCI InsightSeptember 22, 2021Lilian Varricchio et al.assay. TGF-β1, TGF-β2, and TGF-β3 levels were quantitated using commercially available ELISA kits (DB100B, DB250, DY008, R&D Systems). Briefly, supernatants from ND or MF MNC MKs were processed and the levels of TGF-β

(15) A static magnetic field changes the expression profile of the transforming growth factor β family genes in human cells that have been treated with fluoride ionsCytokineJuly 1, 2021Kimsa-Dudek M, Synowiec-Wojtarowicz A, Krawczyk A, Kruszniewska-Rajs C, Gola J.

(16) Desmoglein-2 harnesses a PDZ-GEF2/Rap1 signaling axis to control cell spreading and focal adhesions independent of cell-cell adhesionScientific reportsJune 24, 2021Shelton WT, Thomas SM, Alexander HR, Thomes CE, Conway DE, Dubash AD.

(17) Role of epidermal growth factor receptor inhibitor-induced interferon pathway signaling in the head and neck squamous cell carcinoma therapeutic responseJournal of translational medicineJanuary 23, 2021Korpela SP, Hinz TK, Oweida A, Kim J, Calhoun J, Ferris R, Nemenoff RA, Karam SD, Clambey ET, Heasley LE.

(18) Determining the effect of ellagic acid on the proliferation and migration of pancreatic cancer cell linesTranslational Cancer ResearchJanuary 1, 2021Ji Yoon Kim, Yun Ju Choi, Ha-Jeong Kimat each time. Enzymes-linked immunosorbent assay (ELISA) The concentrations of TGF-β (R&D System, DB250), MMP-2 (R&D System, DMP2F0), and MMP-9 (R&D System, DMP900) in the culture supernatant were measured

(19) Age-induced accumulation of methylmalonic acid promotes tumour progressionNatureSeptember 1, 2020Gomes AP, Ilter D, Low V, Endress JE, Fernández-García J, Rosenzweig A, Schild T, Broekaert D, Ahmed A, Planque M, et al.

(20) Acidosis-Induced TGF-β2 Production Promotes Lipid Droplet Formation in Dendritic Cells and Alters Their Potential to Support Anti-Mesothelioma T Cell ResponseCancersMay 19, 2020Trempolec N, Degavre C, Doix B, Brusa D, Corbet C, Feron O.Measurements Active human TGF-β2 protein levels were assessed by using a dedicated ELISA detection kit (#DB250, R&D Systems, Minneapolis, MN, USA) as previously described [36]. Of note, TGF-β2 present in the conditioned

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Human TGF-beta 2 Quantikine ELISA Kit

TGF-beta 2 ELISA

Human TGF-beta 2 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human TGF-beta 2 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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