Anti-U2AF35/U2AF1 antibody ab86305 from Abcam

Product Specs
ItemAnti-U2AF35/U2AF1 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab86305
ApplicationsIHC-P, IP, WB
Conjugate/TagUnconjugated
IsotypeIgG
Quantity100 µL
ClonalityPolyclonal
HostRabbit
Formatab86305 was affinity purified using an epitope specific to U2AF35 immobilized on solid support
Concentration0.2 mg/ml
Gene NameU2AF1
ReactivityHuman, Mouse
NCBI Gene AliasesU2AF1L5
Antibody TypePrimary Antibody
TargetU2AF1

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United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Activation of ERβ hijacks the splicing machinery to trigger R-loop formation in triple-negative breast cancerProceedings of the National Academy of SciencesMarch 26, 2024Dongfang Wang, Muya Tang, Peidong Zhang, Kailin Yang, Liang Huang, Mengrui Wu, Qiuhong Shen, Jing Yue, Wei Wang, Yanqiu Gong, et al.are as follows: for IF, IHC, and western blot: ERβ (1:2,000; Abcam, ab187291), U2AF1 (1:500; Abcam, ab86305), γH2A.X (1:500; Sigma-Aldrich, 05-636), S9.6 (1:500; Kerafast, ENH001), J2 (1:200; Abcam, ab288755),

(2) A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complexCell chemical biologyAugust 19, 2021Chatrikhi R, Feeney CF, Pulvino MJ, Alachouzos G, MacRae AJ, Falls Z, Rai S, Brennessel WW, Jenkins JL, Walter MJ, et al.(Millipore-Sigma, Cat. No. U4758), H2B (Cell Signaling Technology Inc., Cat. No. 12364), and U2AF1 (Abcam, Cat. No. ab86305) were diluted in blocking solution at 1:1000 (v/v) ratio. Anti-DYKDDDDK was diluted at 1:2000 (v/v)

(3) Prevalent intron retention fine‐tunes gene expression and contributes to cellular senescenceAging CellDecember 4, 2020Jun Yao, Dong Ding, Xueping Li, Ting Shen, Haihui Fu, Hua Zhong, Gang Wei, Ting Niprotein was incubated at 95°C for 10 min before performing SDS‐PAGE. The primary antibodies for U2AF1 (ab86305; 1: 2000, Abcam), CPNE1 (ab155675, 1: 1000, Abcam), and GAPDH (AB2000, 1: 5000, Abways) were incubated

(4) mTOR-regulated U2af1 tandem exon splicing specifies transcriptome features for translational controlNucleic acids researchNovember 4, 2019Chang JW, Yeh HS, Park M, Erber L, Sun J, Cheng S, Bui AM, Fahmi NA, Nasti R, Kuang R, et al.considered for further analyses. Western blotting Antibodies used in this study include: anti-U2AF1 (ab86305, Abcam), anti-U2AF2 (sc-48804, Santa Cruz Biotechnology), anti-hnRNP A1 (4B10, Abcam), anti-RPS6 (#2317,

(5) PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRMNucleic acids researchJuly 6, 2018Královicová J, Ševcíková I, Stejskalová E, Obuca M, Hiller M, Stanek D, Vorechovský I.B-2, Santa Cruz), anti-RBM39 (rabbit polyclonal, HPA001591 Sigma), anti-U2AF35 (rabbit polyclonal, AB86305, Abcam), anti-U1-70K (rabbit polyclonal AV40276, Sigma), anti-U1C (rabbit monoclonal, ab192028, Abcam)

(6) Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activationGenes & developmentOctober 1, 2015Martinez NM, Agosto L, Qiu J, Mallory MJ, Gazzara MR, Barash Y, Fu XD, Lynch KW.(#9271), SAPK/JNK (#9252), and Flag (#2368) from Cell Signaling. Antibodies to hnRNPL (ab6106) and U2AF35 (ab86305) were from Abcam. Antibody to c-myc (9E10) was from Roche, and CELF2 antibody was from the University

(7) Disease-associated mutation in SRSF2 misregulates splicing by altering RNA-binding affinitiesProceedings of the National Academy of SciencesAugust 25, 2015Jian Zhang, Yen K. Lieu, Abdullah M. Ali, Alex Penson, Kathryn S. Reggio, Raul Rabadan, Azra Raza, Siddhartha Mukherjee, James L. Manleytag (G166; ABM), anti-ACTIN (A2066; Sigma), antiSF3B1 (A300-996A; Bethyl Laboratories), anti-U2AF1 (ab86305; Abcam), and anti-snRNP70 (sc-9571; Santa Cruz). Immunoblotting was performed either with LI-COR secondary

(8) Mechanisms for U2AF to define 3' splice sites and regulate alternative splicing in the human genomeNature structural & molecular biologyNovember 1, 2014Shao C, Yang B, Wu T, Huang J, Tang P, Zhou Y, Zhou J, Qiu J, Jiang L, Li H, et al.primary antibodies were used: anti-U2AF65 (U4758, Clone MC3, Sigma-Aldrich, 1:10,000), anti-U2AF35 (ab86305, Abcam, 1:2,000), anti–β-actin (A1978, Clone AC-15, Sigma-Aldrich, 1:20,000), anti-GAPDH (10494-1-AP

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Anti-U2AF35/U2AF1 antibody from Abcam

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Anti-U2AF35/U2AF1 antibody from Abcam

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