Fig 2: Effect of CYB5 concentration on maximum reaction velocities of CYP1A2 (A), 2A6 (B) and 3A4 (C) when sustained by WT CPR and the three hinge mutants. Activities were normalized to the one without CYB5 for each CPR mutant in comparison to WT.

Fig 3: Relative CYP-reaction velocities (kobs) of CYP1A2, 2A6 and 3A4 in function of the NaCl concentration, when combined with CPR WT (A) or mutants S234P (B), I245P (C) and R246A (D). Velocities were normalized with the one observed when combined with WT CPR at 0 M NaCl.

Fig 4: CYP reaction velocity (kobs) in function of the NaCl concentration, for the WT and mutant forms of CPR with CYP1A2 (A), CYP2A6 (B) or CYP3A4 (C).