Fig 1: LIM domain proteins identified in adhesion complexes from MSCs. (A) Protein interaction network of all LIM proteins identified by MS and proteins with which they reportedly directly interact in the adhesome. The non-adhesome LIM protein PDLIM1 did not map on to the interaction network database. (B) Immunofluorescence images of MSCs spread on glass or polyacrylamide-coated coverslips with defined stiffness (12 kPa, Matrigen) previously coated with FN (20 µg/mL). MSCs were cultured for 24 h before fixation with 4% (w/v) paraformaldehyde and visualised using antibodies against PDLIM1 (ab64971, Abcam), PDLIM5 (E-25 and JK-3R, Santa Cruz Biotechnology) and PDLIM7 (ab86065, Abcam) and vinculin (hVIN-1; Sigma-Aldrich). Confocal microscopy images were collected on a Leica TCS SP5 AOBS upright confocal using a 20×/0.50 HCX Apo L water objective and processed using ImageJ.
Supplier Page from Abcam for Anti-PDLIM7 antibody