Immunofluorescent Staining: BALB/c spleen cells were activated by incubation for 72 hours with LPS. The cells were then harvested and stained with anti-Mouse CD86-FITC. A wide gate was set to include small, medium and large cells, following which the cells were analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 1 ug/10e6 cells.