Immunohistochemical analysis of ACE-2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47488) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Western Blot was performed using Anti-ACE2 Polyclonal Antibody (Product # PA5-47488) and a 120 kDa band corresponding to Angiotensin-converting enzyme 2 was observed across all tissues tested except Mouse Skeletal Muscle, Rat Heart, Rat Testis and Rat Skeletal Muscle. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Kidney (Lane 2), Mouse Testis (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Brain (Lane 5), Rat Heart (Lane 6), Rat Kidney (Lane 7), Rat Testis (Lane 8) and Rat Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).