Western blot was performed using Anti-CEACAM1 Polyclonal Antibody (Product # PA5-47842) and a 110 kDa band corresponding to CEACAM1 was observed across tissues tested. Tissue extracts (30ug lysate) of Mouse Liver (Lane 1), Mouse Small Intestine (Lane 2) and Mouse Lung (Lane 3) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/ml) and detected by chemiluminescence with Rabbit anti-Sheep IgG (H+L) Secondary Antibody, HRP (Product # 61-8620, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).