Immunocytochemical analysis of ELF3 was detected in immersion fixed A431 human epithelial carcinoma cell line using 10 µg/mL human/mouse ELF3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47776) for 3 hours at room temperature. Cells were stained with the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue).

Western blot analysis of ELF3 in PC‚3 human prostate cancer cell line, A549 human lung carcinoma cell line, and NIH‚3T3 mouse embryonic fibroblast cell line . Samples were incubated in ELF3 polyclonal antibody (Product # PA5-47776) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions.