Immunohistochemical analysis of IL-17F in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated with IL-17F polyclonal antibody (Product # PA5-46972) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular space and plasma membrane.