Synapsin I Antibody

Product Specs
ItemSynapsin I Antibody - Azide Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberNB300-104-100ul
IsotypeIgG
Research AreaCircadian Rhythm, Cognition and Behavior, Neuronal Cell Markers, Neuroscience, Neurotransmission, Pain Research, Sensory Systems, Vision
Molecular Weight78 kDa
PurityAntigen Affinity-purified
ReactivityHu, Ms, Rt, GP, Pr
ClonalityPolyclonal
NCBI Gene AliasesSYNI, EPILX, EPILX1, MRX50, SYN1b, SYN1a
NCBI Full Gene Namesynapsin I
ApplicationsWB, ICC, IF, IHC, IHC-fr, IHC-p, IP
ImmunogenNative protein purified from bovine brain. Accession # P17599
HostRabbit
TargetSynapsin I
Format10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Conjugate/TagUnconjugated
Quantity100 ul
Antibody TypePrimary Antibodies
StorageStore at -20C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Specific for endogenous levels of the ~78 kDa synapsin I doublet. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

Sizes Available: 100 ul

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Questions and Answers

Can this Synapsin I antibody be used for immunocytochemistry in human-derived neuronal models?

Yes, the Synapsin I antibody has been utilized in immunocytochemistry for human-induced pluripotent stem cell-derived neurons, as demonstrated in studies where it was used to validate neuronal differentiation and assess synaptic markers ().

What is the recommended dilution for using this antibody in Western blot applications?

The Synapsin I antibody is recommended to be used at a dilution of 1:1000 for Western blot applications, as indicated in various protocols ().

Is this antibody effective for detecting synapsin I in rodent models?

Yes, the Synapsin I antibody has been shown to effectively detect synapsin I in rodent models, including studies that utilized it to analyze synaptic structures in the mouse cerebellum ().

What types of samples can this antibody be applied to for immunohistochemistry?

The Synapsin I antibody can be applied to various samples for immunohistochemistry, including brain tissues from mice and human-derived neuronal cultures, as evidenced by its use in studies focusing on synaptic localization and neurogenesis (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Targeting Tumour Microtubes to Disrupt Glioma NetworksResearch SquareApril 7, 2026Tengfei Huang, Tengfei Huang, Suchet Zhang, Suchet Taori, Donghai Wang, Donghai Wang, Huairui Chen, Huairui Yuan, Tingting Duan, Tingting Duan, et al.AB_10001083), PSD95 (Mouse, NOVUS, Cat# NB300–556, 1:1000, RRID: AB_2092366), SYNAPSIN-1 (Rabbit, NOVUS, Cat# NB300–104, 1:1000, RRID: AB_10078308), GLUR1 (Rabbit, NOVUS, Cat# NBP2–22399, 1:1000, RRID: AB_2857850), TOMM20

(2) Low oral dose of 4-methylumbelliferone reduces glial scar but is insufficient to induce functional recovery after spinal cord injuryScientific reportsNovember 6, 2023Štepánková K, Chudíčková M, Šimková Z, Martinez-Varea N, Kubinová Š, Urdzíková LM, Jendelová P, Kwok JCF.IgG,Invitrogen cat. no. MA5-12111 Anti-synapsin,Synapsin,Rabbit polyclonal,Novus biologicals cat. no. NB300-104 Anti-Nestin,Nestin,Mouse monoclonal IgG1,Chemiconcat. no. MAB353 Anti-NG2,Neural/glial antigen 2,Rabbit

(3) Treadmill Exercise Alleviates Cognition Disorder by Activating the FNDC5: Dual Role of Integrin αV/β5 in Parkinson's DiseaseInternational journal of molecular sciencesApril 25, 2023Tang C, Liu M, Zhou Z, Li H, Yang C, Yang L, Xiang J.ab112, Abcam, Shanghai, China), PSD95 (1:1000, 3450S, CST, Fall River, MA, USA), Synapsin (1:1000, NB300-104, NOVUS (Shanghai) Co. Ltd, Centennial, CO, USA), CAMKII(1:1000, 50049S, CST, Fall River, MA, USA), SNAP47

(4) Differentiating PC12 cells to evaluate neurite densities through live-cell imagingSTAR protocolsMarch 17, 2023Karliner J, Merry DE.AB_794335 "Synapsin-I antibody (rabbit), 1:1000 for Western blot and immunocytochemistry",Novus Biologicals,Cat#NB300-104; RRID: AB_10078308; Lot#ajo919p "Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody,

(5) Antibody stabilization for thermally accelerated deep immunostainingNature methodsSeptember 1, 2022Lai HM, Tang Y, Lau ZYH, Campbell RAA, Yau JCN, Chan CCY, Chan DCW, Wong TY, Wong HKT, Yan LYC, et al.

(6) Neuronal Glypican4 promotes mossy fiber sprouting through the mTOR pathway after pilocarpine-induced status epilepticus in miceExperimental neurologyJanuary 1, 2022Ma KG, Hu HB, Zhou JS, Ji C, Yan QS, Peng SM, Ren LD, Yang BN, Xiao XL, Ma YB, et al.

(7) Exercise protects from hippocampal inflammation and neurodegeneration in experimental autoimmune encephalomyelitisBrain, behavior, and immunityNovember 1, 2021Rizzo FR, Guadalupi L, Sanna K, Vanni V, Fresegna D, De Vito F, Musella A, Caioli S, Balletta S, Bullitta S, et al.

(8) Dysregulated APP expression and α-secretase processing of APP is involved in manganese-induced cognitive impairmentEcotoxicology and environmental safetySeptember 1, 2021Yang Y, Zhang J, Yang X, Li Z, Wang J, Lu C, Nan A, Zou Y.

(9) A novel structure associated with aging is augmented in the DPP6-KO mouse brainActa neuropathologica communicationsNovember 23, 2020Lin L, Petralia RS, Lake R, Wang YX, Hoffman DA.IHC) Rabbit Wako 019-19741 1:800 MAP-2 Mouse Millipore MAB-3418 1:500 MAP-2 Rabbit Millipore Ab-5622 1:500 MAB377 NeuN Mouse Millipore NeuN Rabbit MilliporeSigma ABN78 1:1000 1:500 Synapsin I Rabbit Novus NB300-104 1:200 Synaptophysin Mouse Sigma S-5768 1:500 PHF-Tau at Mouse Thr231 (AT180) Thermo Fisher MN1040 1:200 Ubiquitin mouse Abcam VGLUT1 Guinea pig

(10) Synaptic Loss in Multiple Sclerosis Spinal CordAnnals of neurologySeptember 1, 2020Petrova N, Nutma E, Carassiti D, Rs Newman J, Amor S, Altmann DR, Baker D, Schmierer K.

(11) hiPSC-Derived Neurons Provide a Robust and Physiologically Relevant In Vitro Platform to Test Botulinum NeurotoxinsFrontiers in pharmacologyJanuary 1, 2020Lamotte JD, Roqueviere S, Gautier H, Raban E, Bouré C, Fonfria E, Krupp J, Nicoleau C.Kingdom ; Ab41489, Abcam ; BLE801202, BioLegend, San Diego, CA, USA), SMI-32 (ab8135, Abcam), SYN (NB300-104, Novus Biologicals, Centennial, CO, USA), SNAP25 (S9684, Sigma-Aldrich), GABA (A0310, Sigma-Aldrich),

(12) Non-proliferative neurogenesis in human periodontal ligament stem cellsScientific reportsDecember 2, 2019Bueno C, Martínez-Morga M, Martínez S.Biolegend), Connexin-43 (3512; 1/300, Cell Signalling), Synaptophysin (18-0130; 1/300, Zymed), Synapsin1 (NB300-104; 1/300, Novus), Fibrillarin (ab5821; 1/300, Abcam) and Lamin A/C (GTX101127; 1/300, GeneTex) in PBS

(13) A slow transcription rate causes embryonic lethality and perturbs kinetic coupling of neuronal genesThe EMBO journalMay 2, 2019Maslon MM, Braunschweig U, Aitken S, Mann AR, Kilanowski F, Hunter CJ, Blencowe BJ, Kornblihtt AR, Adams IR, Cáceres JF.number Antibodies,Antibodies,Antibodies Tuj1,BioLegend,801213 Map2,Millipore,MAB3418 RNAPII (8WG16),Abcam,ab817 Syn1,Novus Biologicals,NB300‐104 Tubulin,Sigma,T9026 NeuN,Abcam,Ab177487 Nestin,Abcam,Ab24692 Chemicals,Chemicals,Chemicals "5,6‐dichlorobenzimidazole

(14) Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissuesNature communicationsMarch 14, 2018Lai HM, Liu AKL, Ng HHM, Goldfinger MH, Chau TW, DeFelice J, Tilley BS, Wong WM, Wu W, Gentleman SM.Dako,M0762 Synapsin I,Rabbit,Polyclonal,Native protein purified from bovine brain,Novus biologicals,NB300-104 Tau (AT8; Phospho-PHF-tau pSer202 + Thr205),Mouse,Monoclonal (IgG1),Partially purified human PHF-Tau,ThermoFisher Scientific

(15) Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord InjuryFrontiers in bioengineering and biotechnologyJanuary 1, 2018Vishwakarma SK, Bardia A, Lakkireddy C, Paspala SAB, Khan AA.(ab5694, Abcam, USA, 1:100) and β-tubulin III (ab78078, Abcam, USA, 1:500) from Abcam, UK. Synapsin I (NB300-104, 1:500) and PSD-95 (6G6-1C9, 1:250) antibodies from Novus Biologicals, Southpark Way, A-8, Littleton,

(16) Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recoveryCytotechnologyOctober 1, 2016Gho CG, Schomann T, de Groot SC, Frijns JH, Rivolta MN, Neumann MH, Huisman MA.

(17) Cocaine Self-Administration and Extinction Leads to Reduced Glial Fibrillary Acidic Protein Expression and Morphometric Features of Astrocytes in the Nucleus Accumbens CoreBiological psychiatryAugust 1, 2016Scofield MD, Li H, Siemsen BM, Healey KL, Tran PK, Woronoff N, Boger HA, Kalivas PW, Reissner KJ.

(18) Co-immunoprecipitation with Tau Isoform-specific Antibodies Reveals Distinct Protein Interactions and Highlights a Putative Role for 2N Tau in DiseaseThe Journal of biological chemistryApril 8, 2016Liu C, Song X, Nisbet R, Götz J.

(19) M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the HippocampusPloS oneJanuary 1, 2016Lam CS, Tipoe GL, Wong JK, Youdim MB, Fung ML.

(20) Overexpression of tissue-nonspecific alkaline phosphatase increases the expression of neurogenic differentiation markers in the human SH-SY5Y neuroblastoma cell lineBoneOctober 1, 2015Graser S, Mentrup B, Schneider D, Klein-Hitpass L, Jakob F, Hofmann C.