Fig 1: Brg1 is required for H3K27me3 levels at derepressed developmental regulators. (A) Density of ChIP-seq reads for H3K27ac and H3K27me3±5 kb of the TSS of upregulated genes. Regions are ranked according to H3K27me3 density. Right bar indicates distribution of developmental transcription factors (TFs). Most developmental TFs are marked by H3K27me3 and not by H3K27ac. (B) Example genomic regions with loss of H3K27me3 at derepressed genes. y-axis denotes reads per bin per million. (C,D) (Left) Average ChIP-seq or input signal from control or 4-OHT-treated cultures at promoters of group I genes for (C) H3K27me3 or (D) Suz12. (Right) Box plot of normalized (C) H3K27me3 or (D) Suz12 ChIPseq read density at group I gene promoters (±5 kb of TSS) for day 4 control (blue) or day 4 4-OHT (red). Significance between groups was determined using a two-sided paired Mann–Whitney U-test.