alpha-Fetoprotein/AFP Antibody AF5369 from R&D Systems, a Bio-Techne Brand

Product Specs
Itemalpha-Fetoprotein/AFP Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberAF5369
Quantity100 ug (also 25 ug)
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry
ReactivityMouse
Targetalpha-Fetoprotein/AFP
IsotypeIgG
Conjugate/TagUnconjugated
HostGoat
NCBI Full Gene Namealpha fetoprotein
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse alpha -Fetoprotein/AFP, Lys19-Val605, Accession # P02772
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesHPAFP, AFPD, FETA

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse alpha -Fetoprotein/AFP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with human alpha -Fetoprotein is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Dedifferentiation-Dependent Regeneration of the Biliary Ductal Epithelium in Response to Hepatic Injury in TFF1-Deficient MiceCellsAugust 27, 2025Taisuke Yamamoto, Junpei Yamaguchi, Toshio Kokuryo, Yukihiro Yokoyama, Takashi Mizuno, Shunsuke Onoe, Masaki Sunagawa, Taisuke Baba, Tomoki Ebata"Santa Cruz Biotechnology (Dallas, TX, USA)",sc-6556,goat,1:50 AFP,"R and D Systems (Minneapolis, MN, USA)",AF5369,goat,1:100 Conditions and primary antibodies used for immunohistochemistry and immunofluorescence.

(2) Tumour-specific activation of a tumour-blood transport improves the diagnostic accuracy of blood tumour markers in miceEBioMedicineJuly 1, 2024Schmithals C, Kakoschky B, Denk D, von Harten M, Klug JH, Hintermann E, Dropmann A, Hamza E, Jacomin AC, Marquardt JU, et al.electrotransferred to nitrocellulose membranes and incubated with antibodies raised against mouse-AFP (#AF5369, R&D Systems, Minneapolis, MN, RRID:AB_2258018) and anti-β-actin (#A2066, Sigma–Aldrich/Merck, RRID:AB_476693).

(3) Identification of CD133(+) intercellsomes in intercellular communication to offset intracellular signal deficiteLifeOctober 17, 2023Kaneko K, Liang Y, Liu Q, Zhang S, Scheiter A, Song D, Feng GS.Dako), EpCAM (552370; BD Biosciences), Sox9 (AB5535: Millipore), CD44v6 (GTX75661; GeneTex), and AFP (AF5369; R&D SYSTEMS) were used as primary antibodies. Fluorescent images were taken with a monochrome camera,

(4) Locus-resolution analysis of L1 regulation and retrotransposition potential in mouse embryonic developmentGenome ResearchSeptember 1, 2023Patricia Gerdes, Dorothy Chan, Mischa Lundberg, Francisco J. Sanchez-Luque, Gabriela O. Bodea, Adam D. Ewing, Geoffrey J. Faulkner, Sandra R. Richardsonagainst β-tubulin (Rabbit IgG; Sigma-Aldrich, #T2200), Α-fetoprotein (AFP), (Goat IgG; R&D Systems AF5369), and smooth muscle actin (Mouse IgG; Thermo Fisher Scientific 14976080). Secondary antibodies used

(5) Mixed-Lineage Leukemia 1 Inhibition Enhances the Differentiation Potential of Bovine Embryonic Stem Cells by Increasing H3K4 Mono-Methylation at Active PromotersInternational journal of molecular sciencesJuly 25, 2023Li C, Han X, Wang J, Liu F, Zhang Y, Li Z, Lu Z, Yue Y, Xiang J, Li X.China), anti-TRA-1-60 (MAB4360, Shanghai, China), anti-TRA-1-81 (MAB4381, Shanghai, China), anti-AFP (AF5369, R&D Systems, Minneapolis, MN, USA), anti-SMA (ab5694, Abcam, Cambridge, MU, USA) and anti-GFAP (I1044,

(6) Dominant role of DNA methylation over H3K9me3 for IAP silencing in endodermNature communicationsSeptember 19, 2022Wang Z, Fan R, Russo A, Cernilogar FM, Nuber A, Schirge S, Shcherbakova I, Dzhilyanova I, Ugur E, Anton T, et al.

(7) Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cellsThe EMBO journalAugust 17, 2020Salazar-Roa M, Trakala M, Álvarez-Fernández M, Valdés-Mora F, Zhong C, Muñoz J, Yu Y, Peters TJ, Graña-Castro O, Serrano R, et al.(Roche, 11814460001), Sox2 (Cell Signaling Technology, 3728), alpha-fetoprotein (AFP; R&D Systems, AF5369), Oct4 (Santa Cruz Biotechnology, sc-9081), KI-67 (Master Diagnostica, 0003110QD), Nestin (Millipore,

(8) Microfluidic Multitissue Platform for Advanced Embryotoxicity Testing In VitroAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 3, 2019Boos JA, Misun PM, Michlmayr A, Hierlemann A, Frey O.rabbit, 1:200), the endodermal marker alpha fetoprotein (R&D Systems, Minneapolis, Minnesota, USA, AF5369, goat, 1:200), and the ectodermal marker nestin (Abcam, Cambridge, UK, ab134017, chicken, 1:200) were

(9) Intrathymic Notch3 and CXCR4 combinatorial interplay facilitates T-cell leukemia propagationOncogeneDecember 1, 2018Ferrandino F, Bernardini G, Tsaouli G, Grazioli P, Campese AF, Noce C, Ciuffetta A, Vacca A, Besharat ZM, Bellavia D, et al.

(10) Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor SignalingCancer cellJune 11, 2018Dhar D, Antonucci L, Nakagawa H, Kim JY, Glitzner E, Caruso S, Shalapour S, Yang L, Valasek MA, Lee S, et al.AB_2076194 anti-CD44v6,ABD Serotec (Biorad),Cat# MCA1967; RRID: AB_323213 anti-AFP,R&D Systems,Cat# AF5369; RRID: AB_2258018 anti-Ki67,Genetex,Cat# GTX16667; RRID: AB_422351 anti-F4/80 (Clone: A3-1),Caltag/Thermo Fisher

(11) Mice lacking RAP1 show early onset and higher rates of DEN-induced hepatocellular carcinomas in female micePloS oneJanuary 1, 2018Ferrara-Romeo I, Martínez P, Blasco MA.

(12) Hepatic loss of miR-122 predisposes mice to hepatobiliary cyst and hepatocellular carcinoma upon diethylnitrosamine exposureThe American journal of pathologyDecember 1, 2013Hsu SH, Wang B, Kutay H, Bid H, Shreve J, Zhang X, Costinean S, Bratasz A, Houghton P, Ghoshal K.

(13) Global gene profiling of spontaneous hepatocellular carcinoma in B6C3F1 mice: similarities in the molecular landscape with human liver cancerToxicologic pathologyJune 1, 2011Hoenerhoff MJ, Pandiri AR, Lahousse SA, Hong HH, Ton TV, Masinde T, Auerbach SS, Gerrish K, Bushel PR, Shockley KR, et al.rabbit polyclonal glutamine synthetase (ab49873, 1:5,000; Abcam, Cambridge, MA); goat polyclonal α-fetoprotein (AF5369, 1:1.0 µg/mL; R&D Systems, Minneapolis, MN); and donkey anti-mouse β-actin monoclonal antibody (Sigma-Aldrich,

(14) Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROSCancer cellJanuary 1, 0001"Yuan D, Huang S, Berger E, Liu L, Gross N, Heinzmann F, Ringelhan M, Connor TO, Stadler M, Meister M, et al."

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alpha-Fetoprotein/AFP Antibody

alpha-Fetoprotein/AFP Antibody

alpha-Fetoprotein/AFP Antibody

alpha-Fetoprotein/AFP Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (14)

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alpha-Fetoprotein/AFP Antibody from R&D Systems, a Bio-Techne Brand

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