Rat Anti-Mouse CD25/IL-2Ra-APC Antibody MBS673293 from MyBioSource.com

Product Specs
ItemRat Anti-Mouse CD25/IL-2Ra-APC Antibody
CompanyMyBioSource.com
Price $365.00Supplier Page View Company Product Page
Catalog NumberMBS673293
Quantity0.1 mg
ApplicationsFlow Cytometry, Immunoprecipitation
Conjugate/TagAllophycocyanin (APC)
FormatAllophycocyanin (APC) Conjugate
ReactivityMouse
TargetCD25/IL-2R alpha
IsotypeIgG2b kappa
Clone3C7
HostRat
ImmunogenLow affinity alpha chain IL-2 receptor, Mr 55 kDa (IL-2R alpha)
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.^14 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^2, 3, 15 Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.^4 CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.^5 It is expressed at a higher level on CD4^+CD8^+ thymocytes.^5, 6 It is also expressed on cultured epidermal Langerhans cells.^7 The biochemical features of murine CD25 have been characterized in detail.^8-10 The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4.^2, 11, 12 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone.^2, 13
ClonalityMonoclonal