Anti-RbcL antibody - Rubisco large subunit, form I (rabbit) AS03 037 from Agrisera

Product Specs
ItemAnti-RbcL antibody - Rubisco large subunit, form I (rabbit)
CompanyAgrisera
Price EUR329.00Supplier Page View Company Product Page
Catalog NumberAS03 037
ImmunogenKLH-conjugated synthetic peptide conserved across all known plant, algal and cyanobacterial RbcL protein sequences (form I L8S8 and form II L2), including, Arabidopsis thaliana O03042, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5
ReactivityAgostis stolonifera cv. Penncross, Arabidopsis thaliana, Apium graveolens, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata, Beta vulgaris, Begonia, Bienertia sinuspersici, Brassica napus, Kandelia candel, Camellia sinensis, Cannabis sativa L., Chaetoceros furcellatus, Chlorococcum dorsiventrale, Colobanthus quitensis, Cicer arietinum, Chenopodium quinoa, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Chlorella sorokiniana, Chlorella vulgaris, Coscinodiscus concinnus, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Cynara cardunculus, Emiliana huxleyi, Euglena gracilis, Ficus carica, Fortunella margarita Swingle, Fraxinus mandshurica, Fucus vesiculosus, Gladieria sulphuraria, Glycine max, Gonyaulax polyedra, Gongolaria barbata, Guzmania hybrid, Heterosigma akashiwo, Hevea, Hordeum vulgare, Hypnum cupressiforme, Jatropha curcas, Karenia brevis, Kochia prostrata, Lathyrus sativus, Liquidambar formosana, Malus domestica, Medicago truncatula, Micromonas pusila, Nicotiana benthamiana, Nicotiana tabacum, Panicum virgatum, Petunia hybrida cv. Mitchell, Phaeodactylum tricornutum, Physcomitrium patens, Pisum sativum, olytrichum formosum, Porosira glacialis, Porphyra, Ricinus communis, Robinia pseudoacacia, Rhytidiadelphus squarrosus, Saccharum, Schima superba, Skeletonema costatum, Skeletonema marinoi, Solanum lycopersicum, Spinacia oleracea, lichens, Stanleya pinnata, Symbiodinium, Synechococcus PCC 7942, Synechococcus elongatus UTEX 2973, Rhoeo discolor, Thalassiosira pseudonana, Thermosynechococcus elongatus, Triticum aestivum, Prochlorococcus, Triticum aestivum, dinoflagellate endosymbionts, extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Tisochrysis lutea, Verbascum lychnitis, Vitis vinifera, Quercus ilex
TargetAnti-RbcL
HostRabbit
Quantity50 µl
ApplicationsImmunofluorescence/confocal Immunolocalization, Immunogold, Tissue Printing, Western blot
ClonalityPolyclonal
FormatLyophilized
Research AreaPlant Biology, Photosynthesis
PuritySerum
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Molecular Weight52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)

Specifications/Features

1: 5000 - 10 000 with standard ECL (WB), 1: 800 (TP), immunofluorescence/confocal microscopy (IF), 1: 1000 (IG), 1: 250 for images see Prins et al. (2008), detailed protocol available on request

Add to Compare List

Questions and Answers

Can this antibody be used to study Rubisco in different plant species?

Yes, the RbcL antibody has been shown to work effectively in various plant species, including Arabidopsis thaliana, Hordeum vulgare, Oryza sativa, and Spinacia oleracea, among others. It is particularly suitable for quantifying Rubisco in plant and algal samples.

How has this antibody been utilized in recent research?

The RbcL antibody has been employed in several studies, including immunoblot analyses to detect Rubisco levels in different plant species under various conditions, such as salt stress and photoinhibition of photosystem II. For instance, it was used in the study by Krausko et al. (2022) to assess the abundance of photosynthetic proteins under salt stress conditions.

What are the recommended dilutions for using this antibody in Western blotting?

For Western blotting, the recommended dilution for the RbcL antibody is between 1:5,000 and 1:10,000, depending on the specific experimental setup and detection method used.

Is there any evidence of this antibody's effectiveness in immunofluorescence applications?

Yes, the RbcL antibody has been utilized in immunofluorescence applications, as indicated by its use in studies that involve confocal microscopy to visualize Rubisco localization in plant cells.

Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) A high-throughput heterologous expression platform for plant synthetic biology based on Arabidopsis suspension cellsJournal of Experimental BotanyJuly 10, 2026Lukas Meile, Germán Alonso-Tolo, Zeltia Ferreiro-Eiras, Xi Jiang, Stefan Burén, Luis M Rubiomonoclonal antibody (Santa Cruz sc-9996, 1:2000), and RbcL with a rabbit polyclonal antibody (Agrisera AS03 037, 1:5000). Secondary horseradish peroxidase (HRP)-conjugated antibodies were used in 2% milk powder in

(2) Comparative Proteomics of Plasma and Flagellar Membranes in Chlamydomonas reinhardtiiInt J Mol SciJuly 9, 2026Yiwen Lin, Sheng Yao, Huan Long, Kaiyao HuangRosenbaum PsbA,Rabbit,"1:20,000",Chloroplast (thylakoid),"Agrisera, AS05 084" RbcL,Rabbit,"1:10,000",Chloroplast (stroma),"Agrisera, AS03 037" Arf1,Rabbit,1:1000,Golgi/endosomal,"Agrisera, AS08 325" RSP3,Rabbit,1:2000,Axoneme,Gift from Professor

(3) Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803Plant PhysiologyJuly 2, 2026Jinlu Hu, Kuo Zhao, Yu Chen, Xingwu Ge, Jing Yang, Gregory F Dykes, Jian-Yu Shi, Lu-Ning Liumin. The membrane was immunoprobed using rabbit polyclonal antisera against RbcL (1:5,000; Agrisera AS03 037), CcmK2 (1:5,000; -PhytoAB PHY5336S), or CcmM (1:5,000; PhytoAB PHY5331S) overnight, followed by goat

(4) Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrityNew PhytologistMay 24, 2026Kaijie Zheng, Farnusch Kaschani, Emma C. Watts, Markus Kaiser, Renier A. L. van der HoornSweden), and GFP (Ab6663; Abcam, Cambridge, UK), diluted 1 : 5000 in PBS‐T. Primary antibody against RbcL (AS03037; Agrisera, Vännäs, Sweden) was diluted 1 : 10 000 in PBS‐T. For PPO detection, membranes were further

(5) Carbon dioxide concentration alters cyanobacterial carboxysome encapsulation and redox state in Synechococcus sp. PCC 7002Communications BiologyMay 4, 2026Clair A. Huffine, Catherine G. Fontana, Rosanna L. Garris, Colin Sempeck, Jeffrey C. Cameron, Anton AvramovAfter blocking membranes overnight, membranes were incubated with primary RbcL (Agrisera, cat. no. AS03037) antibodies. Membranes were then incubated with a secondary antibody conjugated to AlexaFluor 488 (Thermo Fisher

(6) Subcellular visualization and quantification of cyanotoxin synthesis in cyanobacteria reveals distinct compartmentationScientific ReportsApril 19, 2026Rubén Morón-Asensio, Rainer Kurmayerpolyclonal antibody (Agrisera AB, Vännä, Sweden) against Rubisco large subunit form I and form II (#AS03 037) linked to goat anti-rabbit IgG (H + L) superclonal secondary antibody Alexa Fluor 488 (#A27034) was

(7) The GreenCut protein TEF6 maintains photosystem II homeostasis under high light by stabilizing FtsH accumulation in Chlamydomonas reinhardtiiPlant CommunicationsApril 13, 2026Heng Yi, Huanling Yang, Song Bin, Xiahe Huang, Jiale Xing, Yingchun Wang, Yongbiao Xue, Wenqiang YangPsbC/CP43 (AS11 1787), PsbB/CP47 (AS04 038), Lhcbm5 (AS09 408), Cyt f (AS06 119), ATPB (AS05 085), RbcL (AS03 037), and PsbO (AS06 142-33) from Agrisera. Anti-α-tubulin (T5168) and anti-FLAG M2 (F1804) antibodies were

(8) Transient molecular chimerism for exploiting xenogeneic organellesNat CommunMarch 24, 2026Yuichiro Kashiyama, Moe Maruyama, Masami Nakazawa, Tsuyoshi Kagamoto, Hiroki Imanishi, Sayaka Yamamoto, Mio Inoue, Ryo Onuma, Goro Tanifuji, Hiroki Ashida, et al.anti-TsRbcS (epitope: RISTALPIEKRSVA, aa 131–144; Eurofins Genomics KK), rabbit polyclonal anti-RbcL (#AS03-037; Agrisera, Vännäs, Sweden), and mouse monoclonal anti-HA (clone 16B12, #901513; BioLegend, San Diego,

(9) Phytochrome B integrates jasmonic acid and warm temperature signaling pathways to regulate cotyledon chloroplast developmentNature CommunicationsMarch 9, 2026Peipei Qi, Junling Huai, Nan Gao, Yuanyuan Yao, Rongcheng LinTransGen), anti-His (HT501, TransGen), anti-GST (ab184804, Abcam), anti-MYC2 (A20774, ABclonal), anti-RbcL (AS03037, Agrisera), anti-RbcS (AS07259, Agrisera), anti-HSP70 (M20041F, Abmart), anti-Lhcb3 (AS01002, Agrisera),

(10) Assembly and Functional Coordination of Two Families of Metabolic Organelles in SalmonellaMicrob BiotechnolFebruary 3, 2026Ping Chang, Mengru Yang, Yu Chen, Tianpei Li, Marie Held, Lu-Ning Liuanti‐CsoS1 (Agrisera, catalogue no. AS14 2760; dilution 1:5000), anti‐CbbL (Agrisera; catalogue no. AS03 037; dilution 1:10,000), followed by incubation with horseradish peroxidase‐conjugated secondary anti‐rabbit

(11) Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymesProceedings of the National Academy of SciencesJanuary 20, 2026Vishalsingh R. Chaudhari, Myat T. Lin, Kevin M. Hines, Maureen R. HansonBiorad) and incubated with primary antibodies against tobacco LSU (rabbit anti-RbcL, Agrisera, cat# AS03 037), cyanobacterial LSU (rabbit anti-Se_RbcL, kind gift from Douglas Orr, Lancaster University, UK), and

(12) Heterologous expression of the cyanobacterial fructose-1,6−/sedoheptulose-1,7-bisphosphatase in Chlamydomonas reinhardtii causes increased cell size and biomass productivity in mixotrophic conditionsAlgal ResearchJanuary 9, 2026Martina Bussola, Federico Perozeni, Maria Meloni, Matteo Pivato, Mirko Zaffagnini, Matteo Ballottariα-PsbC (CP43) (AS11 1787, dilution used 1:2000), α-AtpC (AS08 312, dilution used 1:10000), α-RbcL (AS03 037, dilution used 1:5000), α-Lhcbm5 (AS09 408, dilution used 1:5000), α-CAH3 (AS05 073, dilution used 1:2000).

(13) Structure and encapsulation of carbonic anhydrase within the α-carboxysomeProceedings of the National Academy of SciencesNovember 18, 2025Pei Cing Ng, Oluwatobi Adegbite, Tianpei Li, Arnaud Baslé, Jon Marles-Wright, Lu-Ning Liuthe peptides APRSDQMDRVSGEGK and FANRNVPKPEKPGSK, 1:10,000 dilution), anti-RbcL (Agrisera, Cat No. AS03 037, 1:10,000 dilution), anti-CsoS2-N (synthesized by GenScript, NJ, USA with the RGTRAVPPKPQSQG peptide,

(14) Intercellular compartmentation of trehalose 6-phosphate metabolism in Setaria viridis leavesJournal of Experimental BotanyNovember 17, 2025Tomás Tonetti, Bruno E Rojas, Regina Feil, Camila Seimandi, Leandro E Lucero, Julieta V Cabello, Paula Calace, Mariana Saigo, Stéphanie Arrivault, Mark Stitt, et al.western blots using commercial antibodies raised against the large subunit of Rubisco (RbcL; Agrisera AS03 037; https://www.agrisera.com/) and PEPC (Agrisera AS09 458), as described below for the immunoblotting

(15) High Dose of Nickel Unbalances Carbon Metabolism and Nitrogen Assimilation in Barley (Hordeum vulgare L.)Plants (Basel)September 20, 2025Alessia De Lillo, Ivana De Rosa, Giorgia Capasso, Giorgia Santini, Concetta Di Napoli, Noemi Russo, Ermenegilda Vitale, Stefania Grillo, Sergio Esposito, Simone Landi(from Agrisera, Vännäs, Sweden—unless otherwise noted) vs. RuBisCO large subunit (RbcL—EC 4.1.1.39, AS03 037); D1 protein (PsbA—AS05 084); glutamine synthetase 1 & 2 (EC 6.3.1.2, AS08295); glyceraldehyde-3-P dehydrogenase

(16) Directed evolution of a plant Rubisco chaperone with altered client recognitionProceedings of the National Academy of SciencesSeptember 16, 2025Siyu Li, ByungUk Lee, Yichong Lao, Sirawit Lertwiriyapiti, Xuhui Huang, Tina Wang7.6) at room temperature for 1 h and incubated with primary antibodies (rabbit anti-RbcL; Agrisera AS03 037 and mouse anti-6xHis; ThermoFisher MA1-21315) in TBST (TBS + 0.1% TWEEN-20) at 4 °C overnight. After

(17) Physiological and Molecular Responses of the Flag Leaf Under L-Phenylalanine Ammonia-Lyase InhibitionCellsSeptember 2, 2025Katarzyna Hura, Michał Dziurka, Tomasz Hura, Magdalena Wójcik-Jagła, Andrzej Zieliński(RA, Rubisco activase − Agrisera AS10 700; RbcL, Rubisco large subunit, form I and form II − Agrisera AS03 037). Proteins were precipitated using 10% (w/v) trichloroacetic acid (TCA) containing 0.07% (v/v) β-mercaptoethanol

(18) A fungal effector promotes infection via stabilizing a negative regulatory factor of chloroplast immunityNat CommunJuly 29, 2025Kunqin Xiao, Feng Yang, Wenjing Cui, Anmo Li, Jeffrey A. Rollins, Jinxin Guo, Xinhua Sun, Fengting Wang, Xiaojie Wang, Xun Xu, et al.anti-His (CW0286M, 1:1000; CWBIO), rabbit polyclonal anti-MORF2 (1:1000, this work), rabbit anti-RbcL (AS03037, 1:10000; Agrisera), rabbit polyclonal anti-actin (AS132640, 1:5000; Agrisera), goat anti-mouse IgG-HRP

(19) OsDUF2488 acts synergistically with OsPrx1.1, regulates ROS metabolism and promotes dehydration tolerance in ricePlant Biotechnology JournalJune 17, 2025Dipak Gayen, Sunil Kumar, Pragya Barua, Nilesh Vikram Lande, Subhasis Karmakar, Amit K. Dey, Saurabh Gayali, Tushar Kanti Maiti, Kutubuddin Ali Molla, Snehal Murumkar, et al.incubated with the target primary antibodies (anti‐COX II, Agrisera A S04 053A; anti‐RuBisCo, Agrisera AS03 037; anti‐ Lhcb1, Agrisera AS01 004 and anti‐YCF54, Agrisera AS10936anti‐YCF54) for 2 h at room temperature.

(20) Thylakoid Targeting Improves Stability of a Cytochrome P450 in the Cyanobacterium Synechocystis sp. PCC 6803ACS Synth BiolMarch 21, 2025Sayali S. Hanamghar, Silas Busck Mellor, Lisbeth Mikkelsen, Christoph Crocoll, Mohammed Saddik Motawie, David A. Russo, Poul Erik Jensen, Julie A. Z. ZedlerLaursen,50 dilution 1:5000), anti-PsbA (Agrisera AS05 084, dilution 1:10,000), or anti-RbcL (Agrisera AS03 037, dilution 1:10,000). As secondary antibody, an anti-rabbit horseradish peroxidase-conjugated antibody

Be the First to Write a Review!

Anti-RbcL : Rubisco large subunit, form I (rabbit)

Anti-RbcL antibody - Rubisco large subunit, form I (rabbit) from Agrisera

Description

Specifications/Features

Questions and Answers

Agrisera

Citations (178)

Reviews

Anti-RbcL antibody - Rubisco large subunit, form I (rabbit) from Agrisera

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map