CD133 Antibody - BSA Free NB120-16518 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemCD133 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberNB120-16518
Quantity0.1 mg (also 0.025 mg)
ApplicationsIF/IHC,Flow Cytometry,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry/ Immunofluorescence
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatPBS
ReactivityHuman,Mouse,Porcine,Rat
TargetCD133
HostRabbit
ImmunogenSynthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865)
ClonalityPolyclonal
NCBI Full Gene Nameprominin 1
NCBI Gene AliasesAC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
Antibody TypePrimary Antibodies
IsotypeIgG
Research AreaCellular Markers, Core ESC Like Genes, Hematopoietic Stem Cell Markers, Neuronal Cell Markers, Stem Cell Markers
PurityImmunogen affinity purified
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: CD133 - Hematopoietic Stem Cell Marker

Sizes Available: 0.1 mg (also 0.025 mg)

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Questions and Answers

Can this antibody be used to study glioma stem cells in my research?

Yes, the CD133 antibody has been utilized in studies investigating glioma stem cells. For instance, it was mentioned in research where CD133 expression was correlated with glioma stemness and was used in immunohistochemical staining to assess glioma samples (king2021, wan2020).

Is this antibody effective for detecting CD133 in human tissues?

The CD133 antibody has been shown to effectively detect CD133 in various human tissues, including in studies of meningiomas where it was used to assess the expression levels of cancer stem cell markers (zongyu2018). It has also been cited in studies involving human umbilical cord blood mesenchymal stem cells, indicating its utility in human tissue analysis (bak2018).

What applications has this antibody been used for in scientific research?

The CD133 antibody has been used in several applications, including Western blotting, immunofluorescence, and immunohistochemistry, as evidenced by its use in multiple studies to analyze stem cell markers and their roles in cancer (helweg2022, ma2020, schultea2020).

Does this antibody have any documented specificity for different species?

Yes, the CD133 antibody is reactive with multiple species, including humans, mice, rats, and porcine, making it versatile for comparative studies across different model organisms (as indicated in the product specifications).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Loss of protein phosphatase 1 regulator TIMAP protein triggers EMT in A549 cellsCell Communication and SignalingApril 13, 2026Fanni Szalmás, Lilla Nagy, Peter Bai, Anita Boratkó,Thermo Fisher Scientific #MA5-11828 CD44,1:1000,Abcam #ab157107 CD133,1:1000,Novus Biologicals a Bio-Techne Brand #NB120-16518 Claudin-1 (D5H1D),1:1000,"Cell Signalling Technology #13,255" E-Cadherin (24E10),1:1000,Cell Signalling

(2) Periosteal distraction improves blood flow and regulates inflammatory response to accelerate foot ulcer healing in diabetic ratsJournal of Orthopaedic Surgery and ResearchDecember 24, 2025Zhaosu Zheng, Haiyang Guo, Songjun Wang, Xiaodong Bai, Zhaohui Songrabbit anti-rat CD34 antibody (CD34, Abcam;ab81289, 1:100); rabbit anti-rat CD133 antibody (CD133, Novus;NB120-16518, 1:100). Western blot On day 15, the left (contralateral surgery) calf muscles were extracted proteins

(3) Exploring the Potential of Saphenous Vein Grafts Ex Vivo: A Model for Intimal Hyperplasia and Re-EndothelializationJournal of Clinical MedicineAugust 14, 2024Nur A’tiqah Haron, Mohamad Fikeri Ishak, Muhammad Dain Yazid, Ubashini Vijakumaran, Roszita Ibrahim, Raja Zahratul Azma Raja Sabudin, Hafiza Alauddin, Nur Ayub Md Ali, Hairulfaizi Haron, Muhammad Ishamuddin Ismail, et al.Abcam, Waltham, CA, USA), CD309-1:100 (NBP2-36428, Novus Biologicals, Centennial, CO, USA), VEGFR-1:100 (NB120-16518, Novus Biologicals, Colorado, USA), CD146-1:100 (NBP2-44512, Novus Biologicals, Centennial, CO, USA),

(4) The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYCInternational Journal of Molecular SciencesFebruary 22, 2022Laureen P. Helweg, Beatrice A. Windmöller, Leonie Burghardt, Jonathan Storm, Christine Förster, Nils Wethkamp, Ludwig Wilkens, Barbara Kaltschmidt, Constanze Banz-Jansen, Christian Kaltschmidtused were anti-CD44 (1:400; 156-3C11; Cell Signaling, Frankfurt am Main, Germany), anti-CD133 (1:100; NB120-16518; NovusBio, Bio-Techne, Wiesbaden-Nordenstadt, Germany), anti-Nestin (1:200; MAB5326; Sigma-Aldrich,

(5) Regulation of gliomagenesis and stemness through acid sensor ASIC1aInternational journal of oncologyOctober 1, 2021King P, Wan J, Guo AA, Guo S, Jiang Y, Liu M.were purchased from Cell Signaling Technology, Inc. Rabbit polyclonal anti-CD133 antibody (cat no. NB120-16518) was purchased from Novus Biologicals, LLC. Rabbit polyclonal anti-aldehyde dehydrogenase 1 antibody

(6) Novel Primary Human Cancer Stem-Like Cell Populations from Non-Small Cell Lung Cancer: Inhibition of Cell Survival by Targeting NF-κB and MYC SignalingCellsApril 27, 2021Beatrice A. Windmöller, Morris Beshay, Laureen P. Helweg, Clara Flottmann, Miriam Beermann, Christine Förster, Ludwig Wilkens, Johannes F. W. Greiner, Christian Kaltschmidt, Barbara Kaltschmidtused were anti-CD44 (1:400; 156-3C11; Cell Signaling, Frankfurt am Main, Germany), anti-CD133 (1:100; NB120-16518; NovusBio, Bio-Techne, Wiesbaden-Nordenstadt, Germany), anti-Nestin (1:200; MAB5326; Millipore, Merck,

(7) SERPINB2 Is a Novel Indicator of Cancer Stem Cell Tumorigenicity in Multiple Cancer TypesCancers (Basel)April 8, 2019Na-Hee Lee, Se-Ra Park, Jin Woo Lee, Soyi Lim, Seung-Ho Lee, Seungyoon Nam, Dong Young Kim, Seung Yeon Hah, In-Sun Hong, Hwa-Yong Lee[38], using the primary SERPINB2 (Abcam, MA, USA, ab47742), CD44 (NOVUS, NBP1-48386), CD133 (NOVUS, NB120-16518) ALDH (Abcam, ab23375) antibodies. Alexa Fluor 488-conjugated rabbit IgG (Molecular Probes, Eugene,

(8) JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling PathwayScientific reportsApril 26, 2018Kim MS, Cho HI, Yoon HJ, Ahn YH, Park EJ, Jin YH, Jang YK.distilled water. We used the following antibodies for western blotting: anti-CD133 (Novus Biologicals, #NB120-16518), anti-E-cadherin (Cell signaling, #3195), anti-EpCAM (Cell signaling, #2929), anti-Vimentin (Cell signaling,

(9) Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1α and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma Rat ModelEvidence-based complementary and alternative medicine : eCAMJanuary 1, 0001"Liu J, Dong X, Li Z, Wang G, Zhou Y, Liu C, Wang K, Li L, Liu J."

(10) A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm)American journal of cancer researchJanuary 1, 0001"Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, et al."

(11) Acid-sensing ion channel 3 is a new potential therapeutic target for the control of glioblastoma cancer stem cells growthScientific reportsJanuary 1, 0001"Balboni A, D'Angelo C, Collura N, Brusco S, Di Berardino C, Targa A, Massoti B, Mastrangelo E, Milani M, Seneci P, et al."

(12) The Ubiquitin E3 Ligase FBXO33 Suppresses Stem Cell-Like Properties and Metastasis in Non-Small-Cell Lung Cancer by Promoting Ubiquitination and Degradation of MycFrontiers in bioscience (Landmark edition)January 1, 0001"Wei Q, Liu Z, Zhu J, Jiang W, Xie H, Feng G, Wang K."

(13) "CD133 as a biomarker for putative cancer stem cells in solid tumours: limitations, problems and challenges"The Journal of pathologyJanuary 1, 0001"Grosse-Gehling P, Fargeas CA, Dittfeld C, Garbe Y, Alison MR, Corbeil D, Kunz-Schughart LA."

(14) Implanted adipose-derived stem cells attenuate small-for-size liver graft injury by secretion of VEGF in ratsAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsJanuary 1, 0001"Ma T, Liu H, Chen W, Xia X, Bai X, Liang L, Zhang Y, Liang T."

(15) Prognostic role of CD133 expression in colorectal cancer: a meta-analysisBMC cancerJanuary 1, 0001"Wang K, Xu J, Zhang J, Huang J."

(16) Spontaneous formation of tumor spheroid on a hydrophilic filter paper for cancer stem cell enrichment"Colloids and surfaces. B, Biointerfaces"January 1, 0001"Fu JJ, Zhou Y, Shi XX, Kang YJ, Lu ZS, Li Y, Li CM, Yu L."

(17) "Interactions with Muscle Cells Boost Fusion, Stemness, and Drug Resistance of Prostate Cancer Cells"Molecular cancer research : MCRJanuary 1, 0001"Uygur B, Leikina E, Melikov K, Villasmil R, Verma SK, Vary CPH, Chernomordik LV."

(18) "Delayed blockage of prostaglandin EP₄ receptors can reduce dedifferentiation, epithelial-to-mesenchymal transition and fibrosis following acute kidney injury"Clinical and experimental pharmacology & physiologyJanuary 1, 0001"Abouelkheir M, Shabaan DA, Shahien MA."

(19) Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cellsThe Journal of reproduction and developmentJanuary 1, 0001"Horiguchi K, Yoshida S, Tsukada T, Nakakura T, Fujiwara K, Hasegawa R, Takigami S, Ohsako S."

(20) Date Palm (Phoenix dactylifera) Fruits as a Potential Cardioprotective Agent: The Role of Circulating Progenitor CellsFrontiers in pharmacologyJanuary 1, 0001"Alhaider IA, Mohamed ME, Ahmed KKM, Kumar AHS."

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