VGLUT2 antibody 135 421 from Synaptic Systems GmbH

Product Specs
ItemVGLUT2 antibody
CompanySynaptic Systems GmbH
Price $425.00Supplier Page View Company Product Page
Catalog Number135 421
Quantity100 µg
ApplicationsWB, IP, ICC, IHC, IHC-P, IHC-G
Formatpurified IgG
Reactivityrat, mouse
TargetVGLUT2
IsotypeIgG2a
Hostmouse
Clonalitymonoclonal
Cell Line95E11
Clone95E11
ImmunogenSynthetic peptide corresponding to AA 566 to 582 from rat VGLUT2

Questions and Answers

What species does this antibody work with for immunohistochemistry applications?

The VGLUT2 antibody has been shown to work with mouse and rat tissues in immunohistochemistry applications, as indicated in various studies including those that utilized this antibody at a dilution of 1:1000.

Can this antibody be used in studies related to neurodegenerative diseases?

Yes, the VGLUT2 antibody has been utilized in research examining neurodegenerative conditions, such as in the study of TDP-43 mislocalization in a model of TDP-43 proteinopathy, where it was employed to analyze synaptic alterations in retinal ganglion cells.

How has this antibody been applied in the context of synaptic research?

The VGLUT2 antibody has been used to investigate excitatory synapse composition and function, as demonstrated in studies mapping proteomic compositions at excitatory postsynaptic sites in the cerebellar cortex, highlighting its role in synaptic signaling.

Is there any evidence of this antibody being used in developmental studies?

Yes, the VGLUT2 antibody has been referenced in studies focusing on the developmental patterns of cerebellar granule cells, where it was used to analyze synaptic markers during dendritic refinement and synapse formation.

Synaptic Systems GmbH

Rudolf-Wissell-Str. 28

Goettingen 37079

Germany

Phone: +49-551-50556-0
+49-551-50556-384

Company Profile

Website: http://www.sysy.com/

(1) Synaptic vesicles that store monoamines and glutamate differ in protein compositionSci AdvJuly 24, 2026Hrach Asmerian, Alexia J. Diaz, Hongfei Xu, Juan A. Oses-Prieto, Jacob Alberts, Barathan Gnanabharathi, Anna M. Sanetra, Poulomi Das, Noah Carr, Alma L. Burlingame, et al.whole-brain samples and Covance, AB_291262 for striatal samples) or anti-VGLUT2 (Synaptic Systems, 135421) antibodies for 2 hours at 4°C. Five microliters of antibody was added per 2 ml of vesicle suspension. In

(2) Protocol to study synapse density or volume-SynDOVE-in brain using confocal microscopy and Imaris three-dimensional surface rendering softwareSTAR ProtocApril 2, 2026Nolwazi Z. Gcwensa, Khaliah Y. Long, Arielle F. Manabat, Laura A. Volpicelli-DaleySCR_014329 Imaris,Bitplane,RRID:SCR_007370 ,, Antibodies,Antibodies,Antibodies ,, VGLUT2,Synaptic Systems,135 421 HOMER1,Synaptic Systems,160 006 pSer129-synuclein,Abcam,51253 ,, Other,Other,Other ,, Nikon A1R

(3) Glutamatergic Heterogeneity in the Neuropeptide Projections From the Lateral Hypothalamus to the Mouse Olfactory BulbJournal of Comparative NeurologyJanuary 1, 2026Meizhu Qi, Julia Won, Catherine Rodriguez, Douglas A. Storace(Ab89886),Abcam,Ab89886 Rabbit anti-MCH primary antibody (Ab274415),Abcam,RRID: AB_3101761 Mouse anti-VGLUT2 primary antibody (135421),Synaptic Systems,RRID: AB_2884916 Guinea pig anti-VGLUT1 primary antibody (135304),Synaptic Systems,135304 Mouse

(4) Conditional mGluR5 knockout in glutamatergic pathways disrupts the development of excitatory synaptic transmission onto mouse MNTB neuronsJournal of NeurophysiologyNovember 1, 2025Huimei Wang, Danah Alquraish, Xiaoyan Yu, Yuan Wang, Yong Lu(PBS) and incubated overnight at 4°C with the primary antibodies anti-VGluT2 (Synaptic Systems Cat# 135 421, RRID:AB_2619823; 1:1000) and anti-mGluR5 (Abcam Cat# ab76316, RRID:AB_1523944; 1:500), diluted in PBS

(5) Thalamus enables active dendritic coupling of inputs arriving at different cortical layersNature CommunicationsSeptember 19, 2025Arco Bast, Jason M. Guest, Rieke Fruengel, Rajeevan T. Narayanan, Christiaan P. J. de Kock, Marcel Oberlaenderanti-mCherry, Invitrogen #PA5-34974, Invitrogen #M11217 and mouse anti-VGlut2 antibody, Synaptic Systems #135421) in PB containing 1% NGS for 48 h at 4 °C. Secondary antibodies were diluted 1:500 (goat anti-Rabbit

(6) Microglial activation and hypothalamic structural plasticity in HFD obesity: insights from semaglutide and minocyclineJournal of Lipid ResearchFebruary 1, 2025Xi Rong, Fang Wei, Yuqi Jiang, Qintao Ma, Dongmei Wang, Jie ShenSynaptic Systems, Germany), Alexa Fluor® 488–conjugated anti-CD68 (ab201844, 1:100, Abcam), mouse anti-vGLUT2 (135421, 1:1,000, Synaptic Systems), and mouse anti-vGAT (131011, 1:1,000, Synaptic Systems). BV2 microglia

(7) Excitatory synaptic structural abnormalities produced by templated aggregation of α-syn in the basolateral amygdalaNeurobiology of diseaseSeptember 1, 2024Gcwensa NZ, Russell DL, Long KY, Brzozowski CF, Liu X, Gamble KL, Cowell RM, Volpicelli-Daley LA.Polyclonal,Dilution ,,,,, VGLUT1,Synaptic Systems,"135,304",AB_887878,Guinea Pig/IgG/Poly,1: 1000 VGLUT2,Synaptic Systems,"135,421",AB_2619823,Mouse/IgG/Mono,1: 1000 HOMER1,Synaptic Systems,"160,006",AB_2631222,Chicken/IgG/Poly,1: 1000

(8) Mapping proteomic composition of excitatory postsynaptic sites in the cerebellar cortexFrontiers in molecular neuroscienceJanuary 1, 2024Robinson K, Delhaye M, Craig AM.(rabbit, Thermofisher, Cat: PA1-046, RRID: AB_2287112, 1:250), VGluT2 (mouse IgG2a, Synaptic Systems, Cat: 135421, RRID: AB_2619823, 1:200). All secondary antibodies were goat antibodies, which include: anti-rabbit

(9) Tonotopic differentiation of presynaptic neurotransmitter-releasing machinery in the auditory brainstem during the prehearing period and its selective deficits in Fmr1 knockout miceThe Journal of comparative neurologyDecember 1, 2022Yu X, Wang Y.

(10) Sarm1 knockout modifies biomarkers of neurodegeneration and spinal cord circuitry but not disease progression in the mSOD1^G93A mouse model of ALSNeurobiology of diseaseOctober 1, 2022Collins JM, Atkinson RAK, Matthews LM, Murray IC, Perry SE, King AE.

(11) Vesicular Glutamate Release from Feeder-FreehiPSC-Derived NeuronsInternational journal of molecular sciencesSeptember 11, 2022Baldassari S, Cervetto C, Amato S, Fruscione F, Balagura G, Pelassa S, Musante I, Iacomino M, Traverso M, Corradi A, et al.

(12) Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3NeuronMay 4, 2022Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, et al.

(13) Adult medial habenula neurons require GDNF receptor GFRα1 for synaptic stability and functionPLoS biologyNovember 1, 2021Fernández-Suárez D, Krapacher FA, Pietrajtis K, Andersson A, Kisiswa L, Carrier-Ruiz A, Diana MA, Ibáñez CF.

(14) In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injuryCell stem cellMay 6, 2021Tai W, Wu W, Wang LL, Ni H, Chen C, Yang J, Zang T, Zou Y, Xu XM, Zhang CL.anti-VGAT,Synaptic Systems,Cat# 131011; RRID: AB_887872 Mouse monoclonal anti-VGLUT2,Synaptic Systems,Cat# 135421; RRID: AB_2619823 "Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor

(15) Deletion of BDNF in Pax2 Lineage-Derived Interneuron Precursors in the Hindbrain Hampers the Proportion of Excitation/Inhibition, Learning, and BehaviorFrontiers in Molecular NeuroscienceMarch 26, 2021Philipp Eckert et al.United Kingdom, anti-rabbit, 1:2000, ab11427) and vGluT2 (Synaptic Systems, Germany, anti-mouse, 1:500, 135421) were detected using appropriate Alexa 488 (Molecular Probes, Germany, 1:500, A11001) and Cy3 (Jackson Immuno

(16) Rapid purification and metabolomic profiling of synaptic vesicles from mammalian braineLifeOctober 12, 2020Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL.,Synaptic Systems,"135303, RRID:AB_887875",(1:1500) dilution Antibody,Rabbit monoclonal VGLUT2,Synaptic Systems,"135421, RRID:AB_2619823",(1:1000) dilution Antibody,Rabbit monoclonal calreticulin,Cell Signaling Technology,"12238S,

(17) Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like PhenotypesThe Journal of neuroscience : the official journal of the Society for NeuroscienceApril 1, 2020Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, et al.catalog #13540419), and VGluT2 (1:300; mouse monoclonal [epitope AA 566 –582]; Synaptic Systems; catalog #135421). Antibodies diluted in TBS with 1% BSA were applied and left overnight at 4°C. After 3 ⫻ 5 min washing

(18) Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1Cerebral cortex (New York, N.Y. : 1991)March 1, 2019Wang L, Kloc M, Maher E, Erisir A, Maffei A.

(19) Developmental pattern and structural factors of dendritic survival in cerebellar granule cells in vivoScientific reportsDecember 3, 2018Dhar M, Hantman AW, Nishiyama H.analysis. For pre-synaptic markers, anti-VGLUT (1:1000 diluted in 5% NGS in TBST, Synaptic systems 135–421) and anti-VGAT (1:1000 diluted in 5% NGS in TBST, Synaptic systems 131–003) primary antibodies were

(20) Assembly of Excitatory Synapses in the Absence of Glutamatergic NeurotransmissionNeuronApril 19, 2017Sando R, Bushong E, Zhu Y, Huang M, Considine C, Phan S, Ju S, Uytiepo M, Ellisman M, Maximov A.RRID:AB_2106755" VGAT,Synaptic Systems,"Cat#131003, RRID:AB_887869" Vglut2,Synaptic Systems,"Cat#135421, RRID:AB_2619823" All other antibodies were homemade and were a kind gift from Dr. Thomas C. Südhof,, Bacterial

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VGLUT2 antibody

VGLUT2 antibody

VGLUT2 antibody

VGLUT2 antibody

VGLUT2 antibody

VGLUT2 antibody from Synaptic Systems GmbH

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VGLUT2 antibody from Synaptic Systems GmbH

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