Equivalent amounts of cell lysates (30 ug per lane) of wild-type HT-1080 cells (WT) and RAD23B-Knockdown HT-1080 cells (KD) were separated by SDS-PAGE and immunoblotted with anti-RAD23B monoclonal antibody TA804867 (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and RAD23B-Knockout Hela cells (KO, Cat# LC810196) were separated by SDS-PAGE and immunoblotted with anti-RAD23B monoclonal antibody TA804867. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RAD23B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804867)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RAD23B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804867)