MyBioSource.com's CaMKII (Alpha-Specific) Antibody: PerCP is a Mouse monoclonal antibody. The CaMKII (Alpha-Specific) Antibody: PerCP was generated using Camk2a as the antigen. It reacts with Human, Mouse, Rat, and Bovine. This antibody has been shown to work in applications such as: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, and Western Blot.
Description
Background Info: Detects ~50-60kDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.
Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12)