TFCP2L1 Antibody AF5726 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTFCP2L1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF5726
NCBI Gene AliasesCRTR1, LBP-9, LBP9
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry
IsotypeIgG
HostGoat
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
TargetTFCP2L1
Quantity100 ug (also 25 ug)
NCBI Full Gene Nametranscription factor CP2 like 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human TFCP2L1, Met1-Lys180, Accession # Q9NZI6
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human TFCP2L1 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Hypoxia-induced immortalization of primary cells depends on Tfcp2L1 expressionCell death & diseaseFebruary 28, 2024Otero-Albiol D, Santos-Pereira JM, Lucena-Cacace A, Clemente-González C, Muñoz-Galvan S, Yoshida Y, Carnero A.1:1000 (Origene; TA150014), Oct3/4 1:500 (Santa Cruz; sc-5279), Tfcp2l1 1:1000 (Novus Biologicals; AF5726), goat anti-rabbit IgG (horseradish peroxidase, HRP) 1:5000 (Abcam; ab97051), rabbit anti-mouse IgG

(2) Bat pluripotent stem cells reveal unusual entanglement between host and virusesCellMarch 2, 2023Déjosez M, Marin A, Hughes GM, Morales AE, Godoy-Parejo C, Gray JL, Qin Y, Singh AA, Xu H, Juste J, et al.AF1979), anti-Zic2 (Abcam, ab150404), anti-Tfe3 (Sigma Aldrich, HPA023881), anti-Tfcp2l1 (R&D Systems, AF5726) or anti-Cdx2 (Life Technologies, PA5-20891) in a 1:50 (anti-Oct3/4) or 1:100 dilution (all others).

(3) Revealing cell populations catching the early stages of human embryo development in naive pluripotent stem cell culturesStem cell reportsJanuary 10, 2023Moya-Jódar M, Ullate-Agote A, Barlabé P, Rodríguez-Madoz JR, Abizanda G, Barreda C, Carvajal-Vergara X, Vilas-Zornoza A, Romero JP, Garate L, et al.AB5603), Anti-TFE3 (1:200, Merck – Sigma-Aldrich, HPA023881-100UL), Anti-hTFCP2L1 (1:200, R&D Systems, AF5726), Anti-GATA3 (1:100, Abcam, ab199428), Anti-NANOG (1:50, ab80392, Abcam), Anti-ZSCAN4 (1:100, Origene,

(4) Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive stateScientific reportsOctober 17, 2022Ozaki H, Suga H, Sakakibara M, Soen M, Miyake N, Miwata T, Taga S, Nagai T, Kano M, Mitsumoto K, et al.Cell Signaling; RRID:AB_2650582), SSEA-4 (MAB4304; mouse; 1:2000; Millipore; RRID:AB_177629), TFCP2L1 (AF5726; goat; 1:200; R&D Systems; RRID:AB_2202564), TRA1-60 (MAB4360; mouse; 1:200; Millipore; RRID:AB_2119183),

(5) The transcription factor Tfcp2l1 promotes primordial germ cell-like cell specification of pluripotent stem cellsThe Journal of biological chemistryOctober 1, 2021Zhang M, Ji J, Wang X, Zhang X, Zhang Y, Li Y, Wang X, Li X, Ban Q, Ye SD.monoclonal antibody (1:2000, 200608, Zen-Bio), Prdm14 (1:1000, D121722, BBI), and Tfcp2l1 (1:1000, AF5726, R&D systems). Immunofluorescence staining The cells were fixed in 4% paraformaldehyde for 20 min

(6) Principles of signaling pathway modulation for enhancing human naive pluripotency inductionCell stem cellSeptember 2, 2021Bayerl J, Ayyash M, Shani T, Manor YS, Gafni O, Massarwa R, Kalma Y, Aguilera-Castrejon A, Zerbib M, Amir H, et al.AB_304980 Rabbit anti-H3K27ac,Abcam,Cat# ab4729; RRID: AB_2118291 Goat anti-TFCP2L1,R and D systems,Cat# AF5726; RRID: AB_2202564 Rabbit anti-KLF17,SIGMA,Cat# HPA024629; RRID: AB_1848933 Rabbit anti-TFAP2C,Cell Signaling

(7) Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid OxidationCell stem cellSeptember 3, 2020Khoa LTP, Tsan YC, Mao F, Kremer DM, Sajjakulnukit P, Zhang L, Zhou B, Tong X, Bhanu NV, Choudhary C, et al.#AF2729, 1:100), Goat anti-OTX2 (R & D Systems, #AF1979, 1:50), Goat anti-TFCP2L1 (R & D Systems, #AF5726, 1:100), Mouse anti-SMA (SIGMA ALDRICH, #A2547, 1:200), Mouse anti-TUJ1 (Biolegend, #801201, 1:200),

(8) Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem CellsCell reportsJune 2, 2020Takahashi K, Jeong D, Wang S, Narita M, Jin X, Iwasaki M, Perli SD, Conklin BR, Yamanaka S.anti-KLF4,Santa Cruz Biotechnology,"sc-20691, RRID:AB_669567" Goat polyclonal anti-TFCP2L1,R&D Systems,"AF5726, RRID:AB_2202564" Rabbit polyclonal anti-TFE3,Sigma-Aldrich,"HPA023881, RRID:AB_1857931" Rabbit polyclonal

(9) Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency RegulationCell reportsMarch 17, 2020Bi Y, Tu Z, Zhang Y, Yang P, Guo M, Zhu X, Zhao C, Zhou J, Wang H, Wang Y, et al.

(10) Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive PluripotencyStem cell reportsDecember 10, 2019Bredenkamp N, Yang J, Clarke J, Stirparo GG, von Meyenn F, Dietmann S, Baker D, Drummond R, Ren Y, Li D, et al.(Santa Cruz, sc-5279), KLF4 (Santa Cruz, sc-20691), KLF17 (Atlas Antibodies, HPA024629), TFCP2L1 (Bio-Techne, AF5726). Antibodies for immunostaining of differentiation markers were: FOXA2 (R&D Systems, AF2400), SOX17

(11) Distinct Molecular Trajectories Converge to Induce Naive PluripotencyCell stem cellSeptember 5, 2019Stuart HT, Stirparo GG, Lohoff T, Bates LE, Kinoshita M, Lim CY, Sousa EJ, Maskalenka K, Radzisheuskaya A, Malcolm AA, et al.anti-T (Brachyury),R&D Systems,Cat#AF2085; RRID:AB_2200235 Polyclonal goat anti-Tfcp2l1,R&D Systems,Cat#AF5726; RRID:AB_2202564 Monoclonal mouse anti-alpha-Tubulin,Abcam,Cat#ab7291; RRID:AB_2241126 ,, "Chemicals,

(12) Honey bee Royalactin unlocks conserved pluripotency pathway in mammalsNature communicationsDecember 4, 2018Wan DC, Morgan SL, Spencley AL, Mariano N, Chang EY, Shankar G, Luo Y, Li TH, Huh D, Huynh SK, et al.

(13) Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus MonkeysCell reportsNovember 27, 2018Kang Y, Ai Z, Duan K, Si C, Wang Y, Zheng Y, He J, Yin Y, Zhao S, Niu B, et al.

(14) Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell FateCell stem cellNovember 1, 2018Sardina JL, Collombet S, Tian TV, Gómez A, Di Stefano B, Berenguer C, Brumbaugh J, Stadhouders R, Segura-Morales C, Gut M, et al.

(15) Trim33 regulates early maturation of mouse embryoid bodies in vitroBiochemistry and biophysics reportsDecember 1, 2017Rajderkar S, Panaretos C, Kaartinen V.Bank MC480), cleaved caspase3 (Cell Signaling 9661), γH2AX (Millipore 05-636-1), Tfcp2l1 (R&D Systems AF5726), Trim33 (Sigma HPA004345), Pou5f1 (Cell signaling #2840) and laminin (Sigma L9393)) were used according

(16) Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent StatesCell stem cellJune 1, 2017Collier AJ, Panula SP, Schell JP, Chovanec P, Plaza Reyes A, Petropoulos S, Corcoran AE, Walker R, Douagi I, Lanner F, et al.performed using Clarity Western ECL reagent (Bio-Rad). Primary antibodies: TFCP2L1 (1:500, R&D Systems AF5726); KLF17 (1:200, Atlas Antibodies HPA024629); POU5F1 (1:500, Santa Cruz sc5279); β-ACTIN (1:1000, Sigma

(17) Resetting transcription factor control circuitry toward ground-state pluripotency in humanCellSeptember 11, 2014Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, Santos F, Clarke J, Mansfield W, et al.NO,Dilution NANOG,Abcam,ab21624,1:200 NANOG,eBioscience,14-5769-82,1:200 KLF4,Santa Cruz,sc-20691,1:400 TFCP2L1,R and D,AF5726,1:500 TFE3,Sigma,HPA023881-100UL,1:500 STELLA,Millipore,MAB4388,1:200 ECAD,Beckman Coulter,IM1763,1:100 CXCR4,BD

(18) α-Intercalated cells defend the urinary system from bacterial infectionThe Journal of clinical investigationJuly 1, 2014Paragas N, Kulkarni R, Werth M, Schmidt-Ott KM, Forster C, Deng R, Zhang Q, Singer E, Klose AD, Shen TH, et al.using Ksp-Cre (Jackson Labs), and the deletion was confirmed by PCR and immunostaining for Tcfcp2l1 (AF5726; R&D Systems). The absence of ICs was detected by immunostaining for H+ATPase (ATP6v1b1; Santa Cruz).

(19) Spatial transcriptional mapping of the human nephrogenic programDevelopmental cellJanuary 1, 0001"Lindström NO, Sealfon R, Chen X, Parvez RK, Ransick A, De Sena Brandine G, Guo J, Hill B, Tran T, Kim AD, et al.",Novus Biologicals,NBP1–49872 anti-Sall1,R&D,PP-K9814–00 anti-Six2,Mybiosource,MBS610128 anti-Six2,ProteinTech,11562–1-AP anti-Sox9,Abcam,ab185230 anti-Tfcp2l1,Novus Biologicals,AF5726 anti-Tfap2a,Cell Signaling,3215 anti-Tfap2b,Cell Signaling,2509S anti-Wt1,Abcam,ab89901 RNA-Scope

(20) "Single-Cell Profiling Reveals Sex, Lineage, and Regional Diversity in the Mouse Kidney"Developmental cellJanuary 1, 0001"Ransick A, Lindström NO, Liu J, Zhu Q, Guo JJ, Alvarado GF, Kim AD, Black HG, Kim J, McMahon AP."Slc5a2 (rabbit, Abcam, ab85626), Rhbg (rabbit, Novus, NBP2–33527), and Tfcp2l1 (goat, R&D Systems, AF5726). Complete information is provided in tabular form in Key Resource Table. RNAscope details RNAscope

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Human TFCP2L1 Antibody

TFCP2L1 Antibody

TFCP2L1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (20)

Reviews

TFCP2L1 Antibody from R&D Systems, a Bio-Techne Brand

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