EAAT2/GLT1 Antibody NBP1-20136 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemEAAT2/GLT1 Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberNBP1-20136
ApplicationsCytometric Bead Assay Standard,IF/IHC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,In-situ Hybridization,In vivo assay,Immunocytochemistry/ Immunofluorescence,In Situ Hybridization
HostRabbit
FormatLyophilized from whole antisera
Quantity0.1 ml (also 0.025 ml)
TargetEAAT2/GLT1
Conjugate/TagUnconjugated
NCBI Full Gene Namesolute carrier family 1 member 2, solute carrier family 1 (glial high affinity glutamate transporter), member 2
ReactivityHu, Ms, Rt, Mk
IsotypeIgG
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
Antibody TypePrimary Antibodies
Research AreaHypoxia, Neuroscience
PurityUnpurified
ClonalityPolyclonal
ImmunogenA synthetic peptide from mouse EAAT2/GLT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human
NCBI Gene AliasesHBGT, GLT-1, EAAT2, EIEE41, Eaat2, GLT1, MGLT1, 1700091C19Rik, 2900019G14Rik, DEE41

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml (also 0.025 ml)

Questions and Answers

Can this antibody be used to study the role of EAAT2 in astrocytes during neurodegenerative diseases?

Yes, the EAAT2/GLT1 antibody has been utilized in studies examining astrocytic functions in neurodegenerative contexts. For instance, it was used in research investigating the modulation of glutamate uptake and release by astrocytes, which is crucial for maintaining glutamate homeostasis and preventing excitotoxicity in conditions such as epilepsy and Parkinson's disease (Liu et al., 2018; Mahmoud et al., 2019).

Is this antibody effective for immunofluorescence applications in human and mouse astrocytes?

The EAAT2/GLT1 antibody has been shown to work effectively in immunofluorescence applications, as evidenced by its use in studies involving both human-induced pluripotent stem cell-derived astrocytes and primary murine astrocytes (Filippini et al., 2021; Mahmoud et al., 2019). It has been employed at a dilution of 1:100 for staining, demonstrating its suitability for visualizing EAAT2 expression in these cell types.

What species can this antibody react with, and how does that impact its utility in research?

This antibody is reactive with mouse, rat, non-human primate, and human species. This broad reactivity allows researchers to apply it in comparative studies across different models of neurodegenerative diseases, enhancing its utility in translational research aimed at understanding the role of EAAT2 in various species (Thomason et al., 2022).

Are there any specific experimental conditions or protocols recommended for using this antibody?

While specific experimental protocols are not detailed in the provided information, the antibody has been successfully used in various applications, including Western blotting and immunohistochemistry, at specified dilutions (e.g., 1:500 for Western blotting). It is advisable to follow standard protocols for these techniques, ensuring proper fixation and permeabilization of cells to achieve optimal staining results (Liu et al., 2018; Schneide et al., 2024).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Ceftriaxone attenuates Poly I:C–induced neuroinflammation in vitro by modulating glutamate transport, synaptic integrity, and immunometabolic reprogrammingFrontiers in Cellular NeuroscienceOctober 28, 2025Xizi Shi, Yesheng Sun, Shirin Hosseini, Fangfang Chen, Thekla Cordes, Kristin Michaelsen-Preusse, Martin KorteEAAT1/GLAST-1/SLC1A3 (rabbit, 1:500, Novus Biologicals, NB100-1869), EAAT2/GLT-1 (rabbit, 1:500, Novus Biologicals, NBP1-20136), GFAP (mouse, 1:1000, Sigma-Aldrich, G3893), GluR1 (GluA1) (guinea pig, 1:400, Alomone Labs, AGP-009),

(2) A phenotypic screening platform for identifying chemical modulators of astrocyte reactivityNature neuroscienceApril 1, 2024Clayton BLL, Kristell JD, Allan KC, Cohn EF, Karl M, Jerome AD, Garrison E, Maeno-Hikichi Y, Sturno AM, Kerr A, et al.(1:1000, BioLegend, 919101), AQP4 (1:100, Sigma Aldrich, HPA014784), GLT-1 (1:100, Novus Biologicals, NBP1–20136), ALDH1L1 (1:100, Novus Biologicals, NBP2–50045), HDAC3 (1:100, BioLegend, 685202), dsRNA (1:500, Millipore,

(3) Using fluorescence activated cell sorting to examine cell-type-specific gene expression in rat brain tissueJournal of visualized experiments : JoVEMay 28, 2015Schwarz JM.

(4) Inhibition of MyD88 Signaling Skews Microglia/Macrophage Polarization and Attenuates Neuronal Apoptosis in the Hippocampus After Status Epilepticus in MiceNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsJanuary 1, 0001"Liu JT, Wu SX, Zhang H, Kuang F."USA, 5 μg/ml), rabbit anti-MR (Abcam, ab64693, Cambridge, MA, USA, 1:500), rabbit anti-GLT-1 (Novus, NBP1-20136, Littleton, CO, USA, 1:100), and rabbit anti-CD163 (Abcam, ab182422, Cambridge, MA, USA, 1:100). After

(5) FACS analysis of neuronal-glial interactions in the nucleus accumbens following morphine administrationPsychopharmacologyJanuary 1, 0001"Schwarz JM, Smith SH, Bilbo SD."

(6) Surface diffusion of astrocytic glutamate transporters shapes synaptic transmissionNature neuroscienceJanuary 1, 0001"Murphy-Royal C, Dupuis JP, Varela JA, Panatier A, Pinson B, Baufreton J, Groc L, Oliet SH."

(7) Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cellsStem cell research & therapyJanuary 1, 0001"Zhang M, Ngo J, Pirozzi F, Sun YP, Wynshaw-Boris A."(Cell Signaling 12,137), NeuN (Abcam ab104225), SOX9 (R&D Systems AF3075), GLT1 (Novus Biologicals NBP1–20136), and GFAP (Dako Z0334) were diluted in blocking buffer and incubated overnight at 4 °C. Following several

(8) Estimating the glutamate transporter surface density in distinct sub-cellular compartments of mouse hippocampal astrocytesPLoS computational biologyJanuary 1, 0001"Rǎdulescu AR, Todd GC, Williams CL, Bennink BA, Lemus AA, Chesbro HE, Bourgeois JR, Kopec AM, Zuloaga DG, Scimemi A."

(9) Deletion of the Sodium-Dependent Glutamate Transporter GLT-1 in Maturing Oligodendrocytes Attenuates Myelination of Callosal Axons During a Postnatal Phase of Central Nervous System DevelopmentFrontiers in cellular neuroscienceJanuary 1, 0001"Thomason EJ, Suárez-Pozos E, Afshari FS, Rosenberg PA, Dupree JL, Fuss B."cells, R&D Systems Cat# IC015P, RRID:AB_2885000), anti-EAAT2/GLT-1 (rabbit polyclonal, 1:50, Novus Cat# NBP1-20136, RRID:AB_1641916), isotype control to anti-EAAT2/GLT-1 (rabbit IgG polyclonal, 1:50, Novus Cat# NBP2-24891,

(10) Direct effect of 2-palmitoyl glycerol on promotion of gamma aminobutyric acid synthesis in normal human fetal-derived astrocytesFEBS open bioJanuary 1, 0001"Tsuboi M, Nakamura Y, Sakuma H."incubated with the following primary antibodies for 2 h at room temperature: rabbit anti‐SLC1A2 (1 : 200, NBP1‐20136, Novus Biologicals, Centennial, USA); rabbit anti‐GAD65/GAD67 (1 : 1000, G5163, Sigma‐Aldrich, Saint

(11) NLRX1 Enhances Glutamate Uptake and Inhibits Glutamate Release by AstrocytesCellsJanuary 1, 0001"Mahmoud S, Gharagozloo M, Simard C, Amrani A, Gris D."washing buffer. Cells were stained with the anti-GLT-1 antibody (Novus Biologicals, Centennial, CO, USA # NBP1-20136) diluted 1:100 or anti-GLAST antibody (Novus Biologicals, Centennial, CO, USA # NB100-1869) diluted

(12) Extracellular clusterin limits the uptake of α-synuclein fibrils by murine and human astrocytesGliaJanuary 1, 0001"Filippini A, Mutti V, Faustini G, Longhena F, Ramazzina I, Rizzi F, Kaganovich A, Roosen DA, Landeck N, Duffy M, et al."(GFAP, GeneTex GTX108711, 1:2,000), anti‐Excitatory Amino Acid Transporter 2 (EAAT2, Novus Biological NBP1‐20136, 1:500). Subsequently, membranes were incubated 1 hr at RT with HRP conjugated secondary antibodies

(13) Distinct forebrain regions define a dichotomous astrocytic profile in multiple system atrophyActa neuropathologica communicationsJanuary 1, 0001"Schneider Y, Gauer C, Andert M, Hoffmann A, Riemenschneider MJ, Krebs W, Chalmers N, Lötzsch C, Naumann UJ, Xiang W, et al."GFAP (Agilent Cat# GA524, RRID:AB_2811722, 1:500) and excitatory amino acid transporter 2 (Novus Cat# NBP1-20136, RRID:AB_2190752, 1:500). Primary antibodies were followed by the incubation of secondary antibodies:

(14) The Effect of Ceftriaxone in Valproic Acid-Induced Mouse Model of AutismAdvanced pharmaceutical bulletinJanuary 1, 0001"Gur G, Topuz RD, Kizilay G."

(15) Interferon-independent STING signaling promotes resistance to HSV-1 in vivoNature communicationsJanuary 1, 0001"Yamashiro LH, Wilson SC, Morrison HM, Karalis V, Chung JJ, Chen KJ, Bateup HS, Szpara ML, Lee AY, Cox JS, et al."

(16) Astrocyte-Secreted Chordin-like 1 Drives Synapse Maturation and Limits Plasticity by Increasing Synaptic GluA2 AMPA ReceptorsNeuronJanuary 1, 0001"Blanco-Suarez E, Liu TF, Kopelevich A, Allen NJ."

Be the First to Write a Review!

EAAT2/GLT1 Antibody - Azide Free

EAAT2/GLT1 Antibody - Azide Free

EAAT2/GLT1 Antibody - Azide Free

EAAT2/GLT1 Antibody - Azide Free

EAAT2/GLT1 Antibody - Azide Free

EAAT2/GLT1 Antibody from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

Citations (16)

Reviews

EAAT2/GLT1 Antibody from Novus Biologicals, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map