Indirect immunostaining of PFA fixed rat hippocampus neurons with anti-calbindin (dilution 1 : 5000; red) and mouse anti-MAP 2 (cat. no. 188 011, dilution 1 : 1000; green). Nuclei have been visualized by DAPI staining (blue).

Indirect immunostaining of the the piriform cortex of rat. Positive cells were revealed with Cy2 and Cy3 conjugated secondary antibodies.
1 anti-calbindin (dilution 1 : 500)
2 anti-parvalbumin (cat. no. 159 004; dilution 1 : 800)
3 overlay

Courtesy: W. Härtig, Paul Flechsig Institute, Leipzig

Indirect immunostaining of paraffin embedded mouse cerebellum section (dilution 1 : 200; red). Nuclei have been visualized by DAPI staining (blue).