Fig 2: Influence of Tregs on the cytokine secretion of T cells. (A) Representative flow cytometry demonstrating the association between Treg quantity and cytokine secretion of T cells in tumors. Comparison of CD4+T cell secretion of (B) IFN-γ, (C) IL-4 and (D) IL-17 and (E) CD8+T cell cytokine secretion between Treg low and Treg high BC patients. All experiments were repeated three times. Treg, regulatory T cell; BC, breast cancer; CD, cluster of differentiation; IFN, interferon; IL, interleukin.

Fig 3: Prognostic ability of CD4, CD8A and FOXP3 mRNA expression in BC patients. Kaplan-Meier survival curves of (A) disease-free survival and (B) overall survival with CD4 expression level. (C) Disease free survival and (D) overall survival with CD8A and (E) disease free survival and (F) overall survival with FOXP3 in BC patients. P-values were calculated using the log-rank method. All experiments were repeated three times. CD, cluster of differentiation; FOXP3, forkhead box protein P3; BC, breast cancer.

Fig 4: Cellular immunogenicity outcomes for adolescents were mostly non-inferior or inconclusive in adolescents in comparison to adults.a, b Adolescents receiving one dose of BNT162b2 (B; N = 58) and 2 doses of BNT162b2 (BB; N = 56) and c adolescents receiving 2 doses of CoronaVac (CC; N = 60) were tested for IFN-γ+ and IL-2+ CD4+ and CD8+ T cells on flow-cytometry-based intracellular cytokine staining assays specific to S (and N and M for CC) for 21 days after dose 1 and 28 days after dose 2. The results of SNM-specific T cell responses were calculated from the sum of responses of the individual S, N and M peptide pools. S-specific IFN-γ+CD4+, IL-2+CD4+ and IFN-γ+CD8+ T cell responses were non-inferior for adolescent BB in comparison to adults (N = 47). For adolescent CC compared to adults (N = 36), SNM-specific IL-2+CD4+, IFN-γ+CD8+ and IL-2+CD8+, N-specific IFN-γ+CD4+, IL-2+CD4+, IFN-γ+CD8+ and IL-2+CD8+, M-specific IFN-γ+CD8+ and IL-2+CD8+ were non-inferior. The remaining cellular immunogenicity outcomes were inconclusive. Dots and error bars show GMR estimates and two-sided 95% CI respectively. GMR geometric mean ratio, CI confidence interval, S spike protein, N nucleocapsid protein, M membrane protein, IFN-γ interferon-γ, IL-2 interleukin-2.

Fig 5: The reciprocal quantity association of CD4, CD8A and FOXP3 mRNA expression. The reciprocal association of (A) CD4 and (B) FOXP3 with CD8A mRNA expression in healthy breast tissue. The reciprocal association of (C) CD4 and (D) FOXP3 with CD8A mRNA expression in BC tissue. All experiments were repeated three times. TPM, transcript per million; CD, cluster of differentiation; FOXP3, forkhead box protein P3; BC, breast cancer.