Rab5 (C8B1) Rabbit mAb 3547S from Cell Signaling Technology

Product Specs
ItemRab5 (C8B1) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3547S
Quantity100 µl
ApplicationsW, IF-IC
ReactivityH, M, R, Mk
TargetRab5
Molecular Weight25
HostRabbit
NCBI Gene AliasesRAB5
ClonalityMonoclonal
NCBI Full Gene NameRAB5A, member RAS oncogene family
CloneC8B1
Research AreaProtein Folding and Trafficking

Questions and Answers

What applications can I use the Rab5 (C8B1) Rabbit mAb for in my experiments?

The Rab5 (C8B1) Rabbit mAb has been shown to work effectively in Western Blotting, Immunocytochemistry, and Immunofluorescence applications, as evidenced by its use in various studies to analyze Rab5 protein levels and localization.

Is this antibody suitable for use in human samples?

Yes, the Rab5 (C8B1) Rabbit mAb is reactive with human samples, as well as mouse, rat, and non-human primate samples, making it versatile for studies involving different species.

How has the Rab5 antibody been utilized in recent research?

The Rab5 antibody has been cited in multiple studies, including its use in immunofluorescence to investigate endosomal trafficking and AKT activation in glioma cells, highlighting its role in understanding cellular signaling pathways and endocytosis mechanisms ().

Can this antibody help in studying endocytosis mechanisms?

Yes, the Rab5 (C8B1) Rabbit mAb is specifically designed to detect Rab5, a key regulator of early endosomal trafficking. Its application in studies has demonstrated its importance in understanding the dynamics of endocytosis and the recruitment of signaling complexes to endosomes ().

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(61) Tight Junction Component Occludin Binds to FIP5 to Regulate Endosome Trafficking and Mitotic Spindle FunctionAdvanced ScienceAugust 1, 2024Zichao Zhang, Jing Chen, Rongze Ma, Chongshen Xu, Yunzhe Lu, Jiecan Zhou, Kun Xia, Pengfei Ludilution), anti‐Rab7 antibody (Cell Signaling, #9367T, 1:200 dilution), anti‐Rab5 antibody (Cell Signaling, #3547T, 1:200 dilution), anti‐γ‐tubulin antibody (Sigma, #T5192, 1:200 dilution), anti‐transferrin receptor

(62) A genetically-encoded fluorescence-based reporter to spatiotemporally investigate mannose-6-phosphate pathwayMolecular Biology of the CellAugust 1, 2024Mallika Bhat, Akshaya Nambiar, Lakshmi Edakkandiyil, Irine Maria Abraham, Ritoprova Sen, Mamta Negi, Ravi ManjithayaSciences "SQSTM1/p62 (ab56416),",Abcam RFP (6G6),ChromoTek "β-tubulin (4466S), GM130 (D6B1) (12480), RAB5 (3547S), LAMP1 (9091P)",Cell Signaling Technology "Anti-mouse IgG conjugated with horseradish peroxidase (HRP)

(63) GGA1 interacts with the endosomal Na+/H+ exchanger NHE6 governing localization to the endosome compartmentJournal of Biological ChemistryAugust 1, 2024Li Ma, Ravi Kiran Kasula, Qing Ouyang, Michael Schmidt, Eric M. Morrow(BD Biosciences, 610822), LAMP1 (Abcam, ab25630), TrkB (BD Biosciences, 610101). Rab5 (Cell Signaling Technology, 3547s and 46449s), Rab7 (Abcam, ab137029 and Cell Signaling Technology, 9367s) and Rab11(BD Biosciences, 610656).

(64) E3 Ubiquitin Ligase RNF13 Suppresses TLR Lysosomal Degradation by Promoting LAMP-1 Proteasomal DegradationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)August 1, 2024Liu W, Wang Y, Liu S, Zhang X, Cao X, Jiang M.anti‐IRF3 (4302S; CST), antibody to phosphorylation IRF3 (4947S; CST), anti‐EEA1 (3288S; CST), anti‐Rab5 (3547S; CST), anti‐Rab11 (5589P; CST), anti‐Rab7b (H00338382‐M01; Abnova), anti‐β‐Actin (M177‐3; MBL), anti‐HA

(65) Melatonergic Signaling Sustains Food Allergy Through FcεRI RecyclingResearch (Wash D C)July 22, 2024Youxia Wang, Xinmei Zhang, Ifen Hung, Chunxue Liu, Wenkai Ren, Liangpeng Ge, Hao WangScientific, A5-115221), ATP1A1 (Proteintech, 55187-1-AP), giantin (Abcam, ab37266), Rab5 (Cell Signaling, 3547), Rab7 (Cell Signaling, 9367), Rab4 (Abcam, ab109009), and Rab11 (Cell Signaling, 5589). Images were

(66) SLIRP promotes autoimmune diseases by amplifying antiviral signaling via positive feedback regulationbioRxivJune 13, 2024Doyeong Ku, Yewon Yang, Youngran Park, Daesong Jang, Namseok Lee, Yong-ki Lee, Keonyong Lee, Jaeseon Lee, Yeon Bi Han, Soojin Jang, et al.,Cell Signaling Technology,Cat# 86298; RRID: AB_2715541 Rabbit monoclonal Rab5,Cell Signaling Technology,Cat# 3547; RRID: AB_2300649 Mouse monoclonal Lamin A/C (E-1),Santa Cruz Biotechnology,Cat# sc-376248; RRID: AB_10991536 Rabbit

(67) EGFR-dependent endocytosis of Wnt9a and Fzd9b is required for β-catenin signaling during hematopoietic stem cell development in zebrafishScience SignalingApril 16, 2024Nicole Nguyen, Kelsey A. Carpenter, Jessica Ensing, Carla Gilliland, Emma J. Rudisel, Emily M. Mu, Kate E. Thurlow, Timothy J. Triche, Stephanie Grainger[Cell Signaling Technologies, 13202S], Fzd9b (8),-Wnt9a (10), α-Rab5, [Cell Signaling Technologies, 3547S], Rab7, [Cell Signaling Technologies, 9367S], Rab9a, [Proteintech, 11420-1-AP], Grp94, [Cell Signaling Technologies

(68) Identification of Four Mouse FcRn Splice Variants and FcRn-Specific VesiclesCellsMarch 29, 2024Haddad G, Blaine J.(abcam, Boston MA, USA cat#ab208943, lot# GR3213103-23), Rab 5 (Cell Signaling, Danvers, MA, USA, cat#3547 (C8B1), lot# 7), Rab7 (Cell Signaling, cat#9367 (D95F2), lot# 3), Rab11 (Cell Signaling, cat#5589 (D4F5),

(69) Single-molecule imaging of stochastic interactions that drive dynein activation and cargo movement in cellsThe Journal of cell biologyMarch 4, 2024Tirumala NA, Redpath GMI, Skerhut SV, Dolai P, Kapoor-Kaushik N, Ariotti N, Vijay Kumar K, Ananthanarayanan V.imaged immediately. The following primary antibodies were used: Rabbit Rab5 Monoclonal Antibody (Cat# 3547, RRID:AB_2300649, 14 µg/ml; Cell Signaling Technology) and Mouse Dynactin 4 Monoclonal Antibody (Cat#

(70) SP-R210 isoforms of Myosin18A modulate endosomal sorting and recognition of influenza A virus infection in macrophagesMicrobes and InfectionMarch 1, 2024Eric Yau, Linlin Yang, Yan Chen, Todd M. Umstead, Anne E. Stanley, E. Scott Halstead, Chintan K. Gandhi, Jonathan W. Yewdell, Zissis C. ChroneosBlocked cells were probed using ras-related protein in brain 5 (Rab5) (Cell Signaling Technology Cat # 3547S, 1:200, Cell signaling Technology Cat#46449S, 1:400) or 7 (Rab7)(Cell Signaling Technology Cat#95746S,

(71) EFHD2 suppresses intestinal inflammation by blocking intestinal epithelial cell TNFR1 internalization and cell deathNature communicationsFebruary 12, 2024Wu J, Xu X, Duan J, Chai Y, Song J, Gong D, Wang B, Hu Y, Han T, Ding Y, et al.#7065), anti-cIAP2 (1:1000, CST, #3130), anti-TRAF2 (1:1000, CST, #4724), anti-Rab5 (1:1000, CST, #3547), anti-EEA1 (1:1000, CST, #3288), anti-p-Cofilin (1:1000, CST, #3313), and anti-Cofilin (1:1000, CST,

(72) The temporal association of CapZ with early endosomes regulates endosomal trafficking and viral entry into host cellsBMC biologyJanuary 25, 2024Zhu H, Wang D, Ye Z, Huang L, Wei W, Chan KM, Zhang R, Zhang L, Yue J.(Proteintech, 25,043–1-AP), His6-tag (ProteinTech, 66,005–1-Ig), α-tublin (Abclonal, AC012), RAB5A (CST, 3547), LAMP1 (CST, 9091), Rab7 (Abcam, ab137029), EEA1 (CST, 3288), Myc-tag (ProteinTech, 67,447–1-Ig), Flag-tag

(73) Key roles of autophagosome/endosome maturation mediated by Syntaxin17 in methamphetamine-induced neuronal damage in miceMolecular MedicineJanuary 3, 2024Xi Wang, Miaoyang Hu, Jingrong Chen, Xinyu Lou, Hongchao Zhang, Muhan Li, Jie Cheng, Tengfei Ma, Jianping Xiong, Rong Gao, et al.China), and the following antibodies were used: anti-LC3A/B (12,741 S), anti-Rab7 (9376T), and anti-Rab5 (3547T) antibodies were from Cell Signaling Technology; anti-p62 (ab209012), anti-Stx17 (ab229646), anti-ATG5

(74) A conserved ion channel function of STING mediates noncanonical autophagy and cell deathEMBO ReportsJanuary 2, 2024Jinrui Xun, Zhichao Zhang, Bo Lv, Defen Lu, Haoxiang Yang, Guijun Shang, Jay Xiaojun Tanfrom Sigma. LAMP1 rabbit mAb (#9091, IF 1:200); GM130 rabbit mAb (#12480, IF 1:300); Rab5 rabbit mAb (#3547, IF 1:200), Phospho-STING S366 (#50907, WB 1:1000); Phospho-TBK1 Ser172 rabbit mAb (#5483, WB/IF 1:1000)

(75) PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferationbioRxivDecember 31, 2023Maria B. Campaña, Madison R. Perkins, Maxwell C. McCabe, Andrew Neumann, Eric D. Larson, Katherine A. FantauzzoCell Signaling Technology, Inc.); Na+/K+-ATPase (1:500; EP1845Y; ab76020; Abcam); RAB5 (1:200; C8B1; 3547; Cell Signaling Technology Inc.); APPL1 (1:200; D83H4; 3858; Cell Signaling Technology Inc.); EEA1 (1:200;

(76) Impact of Targeting Moiety Type and Protein Corona Formation on the Uptake of Fn14-Targeted Nanoparticles by Cancer CellsACS nanoOctober 24, 2023Carney CP, Pandey N, Kapur A, Saadi H, Ong HL, Chen C, Winkles JA, Woodworth GF, Kim AJ.ab109365). Other primary antibodies for immunofluorescence were purchased from CST and included Rab5 (Cat # 3547S), LAMP1 (Cat # 9091S), Clathrin (Cat # 4796S), and Caveolin-1 (Cat # CST 3238S). Anti-rabbit IgG Alexa

(77) Lysosomes mediate the mitochondrial UPR via mTORC1-dependent ATF4 phosphorylationCell discoverySeptember 7, 2023Li TY, Wang Q, Gao AW, Li X, Sun Y, Mottis A, Shong M, Auwerx J.instructions. Cells were then fixed and stained with antibodies to early endosome marker Rab5 (Cat. 3547, CST, 1:200), late endosome marker Rab7 (Cat. 9367, CST, 1:200), or lysosome marker Lamp1 (Cat. 121617,

(78) Cezanne promoted autophagy through PIK3C3 stabilization and PIK3C2A transcription in lung adenocarcinomaCell death discoveryAugust 18, 2023Wang Y, Li J, Zheng H, Wang K, Ren X, Wang G, Du J.anti-PIK3C3 (sc-365404, 1:200), anti-EGFR (sc-373746, 1:200) from Santa Cruz Biotechnology, Anti-Rab5 (3547T, 1:1000) from CST. Anti-Cezanne (16605-1-AP, 1:1000) from Proteintech, anti-GST (TA150101, 1:1000) from

(79) Juvenile CLN3 disease is a lysosomal cholesterol storage disorder: similarities with Niemann-Pick type C diseaseEBioMedicineJune 1, 2023Chen J, Soni RK, Xu Y, Simoes S, Liang FX, DeFreitas L, Hwang R Jr, Montesinos J, Lee JH, Area-Gomez E, et al.for immunoprecipitation (IP) and 1/250 for western blot (WB)], Rab5A (Cell Signaling Technology Cat# 3547, RRID:AB_2300649, 1/1000 for WB), Sec61β (Thermo Fisher Scientific Cat# PA3-015, RRID:AB_2239072, 1/5000

(80) The catalytic inhibitor epacadostat can affect the non-enzymatic function of IDO1Frontiers in ImmunologyApril 14, 2023Eleonora Panfili, Giada Mondanelli, Ciriana Orabona, Marco Gargaro, Claudia Volpi, Maria Laura Belladonna, Sofia Rossini, Chiara Suvieri, Maria Teresa Pallottaclone 19H8 Cat #42575) from Cell Signaling Technology (MA, USA). Anti-Rab5 (rabbit mAb clone C8B1 Cat# 3547) were from Cell Signaling Technology (MA, USA). Phosphatase assay P1.IDO1 and P1.H350A cells were

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3/2/2021Anti-Rab5 Antibody That Works Great for IF Chao YangWe used his antibody to detect GFP-fused proteins in MCF-10A cell line for immunofluorescence staining. It worked very well.

Rab5 (C8B1) Rabbit mAb

Rab5 (C8B1) Rabbit mAb from Cell Signaling Technology

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Rab5 (C8B1) Rabbit mAb from Cell Signaling Technology

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