Fig 1: Western blot validation and network analysis of candidate hSOX10 protein interactors identified by proximity biotinylation and mass spectrometry.(A) Immunoprecipitation (IP)-Western blot validation of six candidate hSOX10 interactors spanning a range of enrichment levels (YAP1, MAML1, ZNF609, TRIM24, STAT3, and KMT2A). Biotinylated proteins purified with streptavidin beads from mT-NLS, mT-hSOX10, and hSOX10-mT expressing cells were probed with protein-specific antibodies. Blotting for hSOX10 is shown as a control to confirm the presence of the bait protein. (B) Network visualization of hSOX10 interactors identified by proximity biotinylation and mass spectrometry. Nodes represent individual proteins, and edges represent known interactions from BioGRID. Node size correlates with mutation frequency in melanoma from cBioPortal data (<1% to 25%), and node color represents the average fold change (FC) enrichment in hSOX10-proximity experiments, with a gradient from green (+5.3) to blue (+10.5). Known hSOX10 interactions are represented by green edges, novel interactions by blue edges, prey-prey interactions by grey edges, and nodes with purple outlines indicate proteins validated by IP-Western blot in panel A. Key complexes, including SWI/SNF and MLL3/4 complexes, as well as transcriptional regulators and additional grouped interactors, are highlighted.