Fig 1: Circ_0001052 played a pro-hypertrophic part in CMs via targeting Hipk3. (A) Overexpression efficiency of Hipk3. (B) The cell surface area in circ_0001052-silenced CMs with up-regulated Hipk3. (C–E). The levels of hypertrophic biomarkers in CMs under diverse contexts. **P < 0.01.
Fig 2: MiR-21 overexpression and HIPK3 down-regulation increase cell viability and decrease LDH activity(A) The cell viability relative to CTL: miR-21 overexpression or HIPK3 down-regulation increased cell viability while miR-21 suppression decreased cell viability (n=3). (B) LDH activity: miR-21 overexpression or HIPK3 down-regulation decreased LDH activity while miR-21 suppression increased LDH activity (n=3 or 4). (C) HIPK3 mRNA expression relative to CTL (n=3). Data were presented as mean ± SD; ##P<0.01 vs. CTL; **P<0.01 vs. H/R+ 21mimic NC; +P<0.05 vs. H/R+ 21inhibitor NC; ++P<0.01 vs. H/R+ 21inhibitor NC; ΔΔP<0.01 vs. H/R+ si-NC.
Fig 3: Schematic abstract revealed the mechanism underlying circ_0001052 regulated Hipk3 in CMs.
Fig 4: MiR-21 interacts with HIPK3(A) Representative immunofluorescence images of HIPK3 expression levels (×400 magnification). (B) Representative images of Western blot analysis. (C) Statistical analysis of HIPK3 levels: miR-21 overexpression inhibited HIPK3 expression, and miR-21 suppression up-regulated HIPK3 expression. The gray values of bands were normalized to that of beta Actin (n=3). (D) MiR-21 expression relative to CTL: transfecting with si-HIPK3 increased miR-21 expression, and transfecting with miR-21 mimic increased miR-21 expression while transfecting with miR-21 inhibitor decreased miR-21 expression (n=3–5). Data are presented as mean ± SD; #P<0.05 vs. CTL; ##P<0.01 vs. CTL; *P<0.05 vs. H/R+ 21mimic NC; **P<0.01 vs. H/R+21mimic NC; +P<0.05 vs. H/R+21inhibitor NC; ++P<0.01 vs. H/R+ 21inhibitor NC; ΔΔP<0.01 vs. H/R+ si-NC.
Fig 5: MiR-21 overexpression and HIPK3 down-regulation suppress apoptosis(A) TUNEL staining showed miR-21 overexpression or HIPK3 down-regulation significantly reduced TUNEL positive cells, and miR-21 suppression increased TUNEL positive cells (×400 magnification). (B) Representative images of flow cytometry: miR-21 overexpression or HIPK3 down-regulation inhibited apoptosis, and miR-21 suppression promoted apoptosis. (C) Quantitative analysis of TUNEL positive cells (n=3). (D) Rates of apoptosis cells detected by flow cytometry (n=3). Data are presented as mean ± SD; ##P<0.01 vs. CTL; **P<0.01 vs. H/R+21mimic NC; ++P <0.01 vs. H/R+ 21inhibitor NC; ΔΔP<0.01 vs. H/R+si-NC.
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