Fig 1: Lineage-Specific Clock Deficiency Ablates Migration Rhythms(A) Numbers of adoptively transferred donor cells present in the blood of control recipients or recipients with Bmal1-deficient endothelial cells 1 hr after transfer at ZT1 and ZT13 (n = 3 or 4 mice; unpaired Student’s t test).(B) Adoptive transfer of donor cells to the liver and lung of control recipients or recipients with Bmal1-deficient endothelial cells 1 hr after transfer at ZT1 and ZT13 (n = 5 mice; unpaired Student’s t test).(C) Expression of endothelial cell ICAM-1 and VCAM-1 in liver and lung of control mice and mice with Bmal1-deficient endothelial cells at ZT1 and ZT13 (n = 4–7 mice; one-way ANOVA).(D) Adoptive transfer of control or Bmal1-deficient B cells to the spleen and lymph node of wild-type recipients 1 hr after transfer at ZT1 and ZT13 (n = 6 mice; unpaired Student’s t test).(E) qPCR analysis of N1rd1 mRNA expression in isolated control and Bmal1-deficient B cells (n = 3 mice; one-way ANOVA).(F) CD11a and CD49d expression on control and Bmal1-deficient B cells in blood at ZT13 (n = 8 mice; unpaired Student’s t test).(G) Adoptive transfer of control or Bmal1-deficient neutrophils to the spleen of wild-type recipients 1 hr after transfer at ZT1 and ZT13 (n = 6 mice; unpaired Student’s t test).(H) qPCR analyses of N1rd1 mRNA expression in isolated control and Bmal1-deficient neutrophils (n = 3 mice; one-way ANOVA).(I) PSGL-1 expression on control and Bmal1-deficient neutrophils in blood at ZT13 (n = 4 or 5 mice; unpaired Student’s t test).*p < 0.05, **p < 0.01, ***p < 0.001. All data are represented as mean ± SEM. ns, not significant. See also Figure S6.