Fibroblast Activation Protein alpha/FAP Antibody

Product Specs
ItemFibroblast Activation Protein alpha/FAP Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $579.00Supplier Page View Company Product Page
Catalog NumberNB110-85534
Quantity0.1 mg
ApplicationsFlow Cytometry,ELISA,Western Blot
Format0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Conjugate/TagUnconjugated
ReactivityHuman,Mouse
PurityImmunogen affinity purified
HostRabbit
TargetFibroblast Activation Protein alpha/FAP
IsotypeIgG
Antibody TypePrimary Antibodies
NCBI Gene AliasesSIMP, DPPIV, FAPA, FAPalpha
ImmunogenThis affinity purified Fibroblast Activation Protein alpha/FAP Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Fibroblast Activation Protein alpha/Fibroblast Activation Protein alpha/FAP protein. (Uniprot: P97321)
NCBI Full Gene Namefibroblast activation protein, fibroblast activation protein alpha
ClonalityPolyclonal
Research AreaCancer, Protein Phosphatase
StorageStore at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for Western blotting in my experiments?

Yes, the Fibroblast Activation Protein alpha/FAP Antibody has been successfully utilized in Western blotting, as demonstrated in studies where it was employed to detect FAP-α in mouse models of Lewis lung carcinoma. The antibody was used at a dilution of 1:1,000 for this application, indicating its effectiveness in protein detection.

Is this antibody specific for mouse fibroblast activation protein?

The Fibroblast Activation Protein alpha/FAP Antibody is specifically reactive with mouse fibroblast activation protein, as indicated by its use in studies involving mouse models. This specificity is crucial for accurately targeting FAP-α expressed in cancer-associated fibroblasts.

What kind of experimental models have utilized this antibody?

This antibody has been used in mouse models, particularly in studies investigating the role of fibroblast activation protein in cancer. For instance, it was employed in research focused on antitumor immunity targeting FAP-α in Lewis lung carcinoma, highlighting its relevance in cancer biology.

Are there any publications that have cited the use of this antibody?

Yes, the Fibroblast Activation Protein alpha/FAP Antibody has been cited in at least four scientific articles, including studies that explore its role in immunotherapy and tumor microenvironments, demonstrating its applicability in current research.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein-α and human livin α exert an antitumor effect against Lewis lung carcinoma in miceImmunity, inflammation and diseaseSeptember 1, 2023Ye Z, Pan J, Yin Z, Wang S, Li Y, Cai X, Zheng H, Cao Z.Beyotime), Western blot was performed by incubating the membranes with primary antibodies against FAP (NB110‐85534, 88 kDa, Novus Biologicals), livin α (NB100‐56145, 33 kDa, Novus Biologicals), CD45 (ab10558, 147 kDa,

(2) Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblastsNature communicationsMay 19, 2022Li X, Yong T, Wei Z, Bie N, Zhang X, Zhan G, Li J, Qin J, Yu J, Zhang B, et al.anti-α-SMA (Novus, cat No NBP2-33006, clone 1A4/asm-1, 1/1000 dilution) and anti-FAP (Novus, cat. No NB110-85534, 1/1000 dilution). Myofibroblasts between passages 3 and 8 were used for the following experiments.

(3) Antitumor immunity targeting fibroblast activation protein-α in a mouse Lewis lung carcinoma modelOncology lettersJuly 1, 2020Xie J, Yuan S, Peng L, Li H, Niu L, Xu H, Guo X, Yang M, Duan F.temperature for 1 h and subsequently incubated with primary antibodies against FAP-α (1:1,000; cat. no. NB110-85534; Novus Biologicals, Ltd.) and GAPDH (1:1,000; cat. no. NB100-56875; Novus Biologicals, Ltd.) for 2 h

(4) VCAM-1 secreted from cancer-associated fibroblasts enhances the growth and invasion of lung cancer cells through AKT and MAPK signalingCancer lettersMarch 31, 2020Zhou Z, Zhou Q, Wu X, Xu S, Hu X, Tao X, Li B, Peng J, Li D, Shen L, et al.

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