Purified anti-DYKDDDDK Tag 637301 from BioLegend

Product Specs
ItemPurified anti-DYKDDDDK Tag
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number637301
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ApplicationsWestern Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA, Flow Cytometry (FC), Purification
Conjugate/TagUnconjugated
IsotypeIgG2a, Lambda
Quantity200 µg
HostRat
CloneL5
Antibody TypePrimary
Concentration0.50 mg/mL
ImmunogenDYKDDDDK-tagged mouse Langerin
ClonalityMonoclonal
ReactivityEpitope tag
TargetDYKDDDDK

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Engineered Bacillus subtilis as oral probiotics to target circulating lactic acidbioRxivDecember 1, 2023Mengdi Yang, Noah Hutchinson, Jianing Yin, Ming Guan, Zongqi Wang, Peiru Chen, Shaobo Yang, Justin D. Crane, Ke Zhang, Jiahe LiThe membranes were incubated with the Anti-FLAG epitope (DYKDDDDK, Biolegend, San Diego, CA, catalog# 637301) or Anti-HA epitope (ABclonal, Woburn, MA catalog# AE008) with 1:2000 dilution overnight in a cold room,

(2) AAV-compatible optogenetic tools for activating endogenous calcium channels in vivoMolecular BrainOctober 17, 2023Yeon Hee Kook, Hyoin Lee, Jinsu Lee, Yeonji Jeong, Jaerang Rho, Won Do Heo, Sangkyu Lee1:2000; Cell Signaling Technologies, Cat# 2250, MA, USA), rat anti-FLAG (diluted 1:500; BioLegend, Cat# 637303, CA, USA), chicken anti-GFAP (diluted 1:1000; Millipore, Cat# AB5541), chicken anti-GFP (diluted 1:1000;

(3) Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platformbioRxiv : the preprint server for biologySeptember 11, 2023Kipfer E, Hauser D, Lett MJ, Otte F, Urda L, Zhang Y, Lang CMR, Chami M, Mittelholzer C, Klimkait T.(Cell Signaling Technology; Cat# 14793; RRID: AB_2572291; LOT# 5), rat monoclonal anti-FLAG (BioLegend; Cat# 637301; RRID: AB_1134266; LOT# B318853), rabbit polyclonal anti-SARS-CoV-2 NSP2 (GeneTex; Cat# GTX135717; RRID:

(4) CLPTM1L is a GPI-anchoring pathway component targeted by HCMVJournal of Cell BiologySeptember 4, 2023Inbal Kol, Ahmed Rishiq, Mevaseret Cohen, Shira Kahlon, Ophir Pick, Liat Dassa, Natan Stein, Yotam Bar-On, Dana G. Wolf, Einat Seidel, et al.(cat. ab129002; Abcam), rabbit α-human CLPTM1L (cat. HPA014791; Sigma-Aldrich), rat α- FLAG tag (cat. 637302; BioLegend), rabbit α-PIGT (cat. 14–266; ProSci), and rabbit α-PIGK (cat. ab201693; Abcam). The α-HIS,

(5) Plant-produced RBD and cocktail-based vaccine candidates are highly effective against SARS-CoV-2, independently of its emerging variantsFront Plant SciAugust 2, 2023Tarlan Mamedov, Damla Yuksel, Irem Gurbuzaslan, Merve Ilgin, Burcu Gulec, Gulshan Mammadova, Aykut Ozdarendeli, Shaikh Terkis Islam Pavel, Hazel Yetiskin, Busra Kaplan, et al.with 1% I-block. The plant-produced antigens were detected with an anti-DYKDDDDK antibody (cat. no. 637301, BioLegend), followed by horseradish peroxidase (HRP)-conjugated anti-rat polyclonal antibody (cat.

(6) Rapid generation of a mouse model for evaluating on-target normal tissue toxicity of human CAR-T cells using replication-defective recombinant adenovirusJournal of advanced researchMay 1, 2023Liao Q, Liu Z, Zhu C, He H, Feng M, Jiang L, Ding X, Sun R, Zhang X, Xu J.for immunohistochemistry (IHC), and immunostained with anti-human CD3 (Cat #: ab11089, Abcam), anti-FLAG tag (Cat #: 637301, Biolegend) antibodies and DAPI for immunofluorescence, which performed by the immunoassay platform

(7) Development of the oncolytic virus, CF33, and its derivatives for peritoneal-directed treatment of gastric cancer peritoneal metastasesJournal for ImmunoTherapy of CancerApril 5, 2023Annie Yang, Zhifang Zhang, Shyambabu Chaurasiya, Anthony K Park, Audrey Jung, Jianming Lu, Sang-In Kim, Saul Priceman, Yuman Fong, Yanghee Woocontrol antibody, Biolegend catalog# 402202, clone#27-35; rat anti-FLAG-tag antibody, Biolegend catalog#637304, clone# L5; rat isotype control antibody, Biolegend catalog#402302, clone#G013C12) for 30 min. After

(8) HDGFRP3 interaction with 53BP1 promotes DNA double-strand break repairNucleic acids researchMarch 21, 2023Zhang Z, Samsa WE, De Y, Zhang F, Reizes O, Almasan A, Gong Z.Santa Cruz. The anti-MBP antibody (#906901), anti-Rat 53BP1 antibody (#933002), anti-Rat Flag antibody (#637301), Alexa Fluor® 488 anti-PCNA (#307909), and Alexa Fluor® 488 anti-γH2AX (#613405) were purchased from

(9) Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike proteinSTAR ProtocolsMarch 1, 2023Angela Rose Liu, Shrikanth C. Basavarajappa, Nandini Sarkar, Anna Bruchez, Parameswaran RamakrishnanMonoclonal anti-HIS tag antibody produced in mouse,Sigma-Aldrich,SAB1305538; RRID: AB_2687993 Anti-DYKDDDDK (FLAG) antibody,BioLegend,637303: RRID: AB_1134265 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, SARS CoV-2

(10) Signal sequences encode information for protein folding in the endoplasmic reticulumThe Journal of cell biologyJanuary 2, 2023Sun S, Li X, Mariappan M.DNA Sequencing). Antibodies and reagents Antibodies used for immunoblotting are as follows: Rat α-FLAG L5 (#637303; BioLegend), Rabbit α-HA, Rabbit α-GFP, Rabbit α-Sec62, Rabbit α-Sec63, Rabbit α-Sec61β, Rabbit α-BAG6,

(11) Mechanism of IFT-A polymerization into trains for ciliary transportCellDecember 1, 2022Shimi Meleppattu, Haixia Zhou, Jin Dai, Miao Gui, Alan Brownincubated with 5 mL blocking buffer containing a 1:500 dilution of anti-FLAG antibody (BioLegend, #637301) overnight with constant shaking at 4°C. Next, the strip was washed three times with 10 mL TBST for

(12) MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactionsPlant MethodsDecember 1, 2022Florent Velay, Mélanie Soula, Marwa Mehrez, Clément Belbachir, Stefano D’Alessandro, Christophe Laloi, Patrice Crete, Ben Fieldantibodies. Primary antibodies targeting the HA tag (monoclonal ab9110, Abcam), FLAG tag (monoclonal 637301, BioLegend) and GFP (polyclonal A-11122, Thermofisher) were all used at a concentration of 1/3000. Biotinylated

(13) RASopathy Mutations Provide Functional Insight into the BRAF Cysteine-rich Domain and Demonstrate the Importance of Autoinhibition in BRAF RegulationMolecular CellNovember 7, 2022Russell Spencer-Smith, Elizabeth M. Terrell, Christine Insinna, Constance Agamasu, Morgan E. Wagner, Daniel A. Ritt, Jim Stauffer, Andrew G. Stephen, Deborah K. Morrisonantibody,Millipore/Sigma,cat# F3165; RRID:AB_439685 FLAG (L5) rat monoclonal antibody,BioLegend,cat# 637304; RRID:AB_1134267 MEK1 mouse monoclonal,BD Biosciences,cat# 61022; RRID:AB_397528 pS217/221-MEK rabbit

(14) Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapyCell reportsNovember 1, 2022Wang W, Wu S, Cen Z, Zhang Y, Chen Y, Huang Y, Cillo AR, Prokopec JS, Quarato G, Vignali DAA, et al.anti-mouse IFN-γ (Clone XMG1.2),BioLegend,"Cat#505821, RRID:AB_961361" Flag(anti-DYKDDDDK),BioLegend,"Cat#637301, RRID:AB_1134266" cGAS (D3O8O) Rabbit mAb (Mouse Specific),Cell Signling,"Cat#31659, RRID:AB_2799008" Cleaved

(15) Multiphase coalescence mediates Hippo pathway activationCellNovember 1, 2022Li Wang, Kyungsuk Choi, Ting Su, Bing Li, Xiaofeng Wu, Ruihui Zhang, Jordan H. Driskill, Hongde Li, Huiyan Lei, Pengfei Guo, et al.tag,Thermo Fisher Scientific,Cat# R960-25; RRID: AB_2556564 Rat monoclonal anti-FLAG,BioLegend,Cat# 637302; RRID: AB_1134268 Mouse monoclonal anti-FLAG-HRP,Sigma,Cat# A8592; RRID: AB_439702 Rabbit polyclonal

(16) The Deubiquitinating Enzyme USP48 Interacts with the Retinal Degeneration-Associated Proteins UNC119a and ARL3International Journal of Molecular SciencesOctober 19, 2022Laura SÃ¡nchez-Bellver, Andrea FÃ©rriz-Gordillo, Marc Carrillo-Pz, Laura Rabanal, Francesc R. Garcia-Gonzalo, Gemma Marfanyanti-HA (BioLegend, San Diego, CA, USA; 901502; 1:250), and anti-FLAG (BioLegend, San Diego, CA, USA; 637301; 1:250). After incubation, cells were rinsed with PBS and incubated with the corresponding secondary

(17) The Phosphofurin Acidic Cluster Sorting Protein 2 (PACS-2) E209K Mutation Responsible for PACS-2 Syndrome Increases Susceptibility to ApoptosisACS OmegaSeptember 27, 2022Rong Xuan Zang, Mitchell J. Mumby, Jimmy D. DikeakosBurlington, ON) for 45 min before overnight incubation with primary antibodies at 4 °C. Anti-FLAG (BioLegend, 637303; WB from 1:10 000 to 1:20 000), anti-HA (MilliporeSigma, H6908; WB 1:1000), and anti-β-actin (Invitrogen,

(18) Role for N-glycans and calnexin-calreticulin chaperones in SARS-CoV-2 Spike maturation and viral infectivityScience advancesSeptember 23, 2022Yang Q, Kelkar A, Sriram A, Hombu R, Hughes TA, Neelamegham S.were from GenScript (Piscataway, NJ). Rat anti-FLAG tag mAb L5 (IgG2a) in unconjugated (catalog no. 637301), Alexa Fluor 647–conjugated (catalog no. 637315, RRID:AB_2716154), and horseradish peroxidase (HRP)–conjugated

(19) Anti-human ACE2 antibody neutralizes and inhibits virus production of SARS-CoV-2 variants of concerniScienceSeptember 16, 2022Abigael E. Chaouat et al.cell types with either anti-hACE2.01-16 (generated by us)/Purified anti-DYKDDDDK Tag Antibody (Cat#637302, BioLegend), /commercial anti-hACE2 (#ABIN1169449, antibodies-online) were performed at 4 °C for 1 h,

(20) Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitmentNeurobiology of diseaseAugust 1, 2022Kluss JH, Bonet-Ponce L, Lewis PA, Cookson MR.1:10,000 for WB), mouse anti-LAMP1 (DSHB, cat #H4B3, 1:100 for ICC), rat anti-FLAG (Biolegend, cat #637302, 1:100 for ICC), chicken anti-GFP (AvesLab, GFP-1020, 1:1,000 for ICC), mouse anti-GFP (Roche, cat #11814460001,

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(1)

6/11/2019Good Purified Anti-DYKDDDDK Tag Antibody from BioLegend Aleksandra WroblewskaHighly recommended! Better than other FLAG antibodies I tried before (such as clone 5A8E5 from GenScript).
(1)

3/6/2019Purified Anti-DYKDDDDK - Great Product Cai HuangThis is the best FLAG antibody I have ever used. Great for WB and IP.
(1)

2/5/2019Sensitive FLAG Antibody For Blotting Needs Pradeep KotaThe product reviewed here is an anti-FLAG antibody that recognizes DYKDDDDK tag either at the N- or C-terminus of target proteins. This antibody was tested in protein detection from lysates resolved ... Read Review

Purified anti-DYKDDDDK Tag from BioLegend

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Purified anti-DYKDDDDK Tag from BioLegend

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