GLI-3 Antibody AF3690 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGLI-3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF3690
NCBI Gene AliasesGCPS, GLI3FL, PAP-A, PAPA1, PAPA, PAPB, PPDIV, ACLS, PHS, GLI3-190
Quantity100 ug (also 25 ug)
ReactivityChicken,Drosophila,Human,Mouse,Transgenic Mouse,Nematode - Caenorhabditis elegans
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene NameGLI family zinc finger 3
ApplicationsWB, ICC, IHC, IHC-p, IP, ChIP, FA
ClonalityPolyclonal
Antibody TypePrimary Antibodies
TargetGLI-3
PurityAntigen Affinity-purified
ConcentrationLYOPH
IsotypeIgG
Conjugate/TagUnconjugated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ImmunogenE. coli-derived recombinant human GLI-3, Met1-Glu479, Accession # P10071

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.

Sizes Available: 100 ug (also 25 ug)

Add to Compare List

Questions and Answers

What applications can I use this GLI-3 antibody for in my experiments?

The GLI-3 antibody has been shown to work effectively in various applications, including Western blotting, immunocytochemistry, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation, and fluorescence assays.

Has this antibody been used in any specific studies related to the Hedgehog signaling pathway?

Yes, the GLI-3 antibody has been utilized in studies investigating the Hedgehog signaling pathway. For instance, it was used in research demonstrating that GLI factors, including GLI-3, are involved in the transcriptional regulation of the SLUG gene in melanoma cells, highlighting its role in cancer biology (see ).

Can this antibody detect GLI-3 in different species?

The GLI-3 antibody is reactive with human, mouse, and C. elegans (worm) GLI-3, making it suitable for studies across these species.

What dilution should I use for this antibody in Western blotting?

In Western blotting applications, the GLI-3 antibody has been used at a dilution of 1:1000, as indicated in various studies (e.g., and ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Microtubule binding protein Togaram1 is required for proper development of mammalian forebrain and neural primary ciliaDevelopmental BiologySeptember 1, 2026Clarissa Q. Nassar, Savera J. Shetty, Noelle D. DwyerTris-buffered saline with 0.1% Tween20 (TBS-T) and incubated with goat anti-Gli3 (R&D Systems, cat #AF3690) at 1:1000 and mouse anti-beta-Actin (Santa Cruz Technologies cat #Sc-47778) at a dilution of 1:5,000

(2) Stable overexpression of Nup88 enhances HeLa cell migration through independent regulation of Kif7 and Gli1Oncol RepJuly 24, 2026Masaki Makise, Ryota Uchimura, Ayano Shiraishi, Akihiko Kuniyasu80031-1-RR), and anti-KRAS (cat. no. 12063-1-AP) from Proteintech Group, Inc.; anti-human/mouse Gli3 (cat. no. AF3690; R&D Systems); anti-phospho-mTOR (Ser2481) (cat. no. 09-343SP) from Merck Millipore. Peroxidase-conjugated

(3) Base editing-derived models of human WDR34 and WDR60 disease alleles replicate retrograde intraflagellar transport (IFT) and hedgehog signaling defectsCommun BiolJuly 1, 2026Dinu Antony, Elif Yilmaz Güleç, Anna Klawonn, Zeineb Bakey, Isabel Schüle, Gwang-Jin Kim, Toni Cathomen, Ilona Skatulla, Han G. Brunner, Sebastian J. Arnold, et al.Massachusetts, USA), followed by western analysis using goat polyclonal GLI3 antibody (1:1000 dilution; AF3690, R&D Systems, Minneapolis, Canada). The intensity of bands was then quantified by Image J (densitometry).

(4) Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalonCell ReportsMay 26, 2026Xiaoliang Liu, Oscar T. Gutierrez, Sabyasachi Baboo, Eva Cai, Gurleen Kaur, Yazan Al-Issa, Jolene K. Diedrich, John R. Yates, Xuecai Gefor WB),Novus Biologicals,Cat#: NBP2-16665 RRID: AB_3263717 Goat anti-GLI3 (1:1000 for WB),R&D Systems,Cat#: AF3690 RRID: AB_2232499 Mouse anti-V5 (1:500 for IF),Thermo Fisher Scientific,Cat#: R960-25 RRID: AB_2556564 "Rabbit

(5) Light- and chemical-induced ciliary signaling governs dorsal/ventral regionalization of human telencephalic organoidsNature CommunicationsMay 22, 2026Issei S. Shimada, Akari Goto, Yutaka Hashimoto, Hajime Inoue, Takuto Sugawara, Tomohiro Doura, Tsubasa Fujita, Toshiaki Iwata, Riko Shimmoto, Hiroshi Takase, et al.(Proteintech, 17711-1-AP), β-actin (Santa Cruz, sc-69879), GAPDH (Novus, NB300-322), GLI3 (R&D Systems, AF3690)}, diluted 1:2500 in 0.1% PBST at 4 °C overnight or Bullet ImmunoReaction buffer (Nacalai Tesque, 18439-85)

(6) Gli2 and Gli3 synergistically mediate HH-TGF-β crosstalk in mesenchymal progenitor cells to orchestrate tooth root morphogenesisInt J Oral SciMarch 12, 2026Tao Zhou, Linyang Huang, Yaxin Xie, Yijia Yin, Yufei Yao, Li Mei, Paul R. Cooper, Hu Zhao, Xianglong Han, Junjun Jingantibodies used for CUT&Tag were: Gli2 (1:50, NB-600-874, Novus Biologicals, USA), Gli3 (5 μg/106 cells, AF3690, R&D systems, USA), rabbit control IgG (1:50, AC005, Abclone, China), goat anti-rabbit IgG H&L (Ab207,

(7) Loss of SPECC1L in cranial neural crest cells results in increased hedgehog signaling and frontonasal dysplasiaFrontiers in PhysiologyJanuary 22, 2026An J. Tran, Brittany M. Hufft-Martinez, Dana N. Thalman, Lorena Maili, Sean A. McKinney, Jeremy P. Goering, Paul A. Trainor, Irfan SaadiKi-67 (1:500, Cell Signaling, 12202), GLI1 (1:100, Cell Signaling, 2553S), GLI3 (1:100, R&D System, AF3690), Non-phospho (Active) β-catenin (1:500, Cell Signaling, 8814S), SOX10 (1:50, Proteintech, 10422-1-AP),

(8) A dual role for GLI3 signaling in neural crest developmentDevelopmentJanuary 1, 2026Simon J. Y. Han, Vinit Adani, Edward Farrow, Bhaval Parmar, Ching-Fang Chang, Kim Cochran, Ronald R. Horne, Paige J. K. Ramkissoon, Ezekiel Esteban, Kelsey H. Elliott, et al.(Cell Signaling Technology, 13202S) before being probed with the following antibodies: GLI3 antibody (1:200; R&D Systems, AF3690) and FLAG M2 antibody (1:1000; Sigma-Aldrich, F1804–200UG). Chick embryo in situ hybridization, IF

(9) Identification of conserved residues essential for the ciliogenic functions of WDPCPDisease Models & MechanismsNovember 1, 2025Yeon Ja Choi, Sungbo Hwang, Chanjae Lee, Huiqing Zeng, Xi Chen, Ukhyun Jo, Hyungjin Kim, Aimin Liu, Daeui Park, John B. Wallingford, et al.at 4°C overnight. These antibodies were as follows: anti-HA (11867423001, Roche, 1:1000), anti-GLI3 (AF3690, R&D Systems, 1:200), anti-GAPDH (2118S, Cell Signaling Technology, Danvers, MA, USA, 1:1000) and anti-β-actin

(10) Smoothened Inhibitor, PF-04449913 Inhibits the Development of Myelofibrosis in a JAK2V617F Transgenic Mouse Model by Reducing TGF-β and MAPK Signaling PathwaysResearch squareMay 9, 2025Merchant A, Chaudhry P, Singh M, Lemos T, Fargal E, Siddiqi I, O'Connell C, Zhao Z.

(11) Proteomic profiling of primary cilia in the developing brain uncovers new regulators of cortical developmentbioRxiv : the preprint server for biologyMay 4, 2025Liu X, Gutierrez OT, Kaur G, Al-Issa Y, Baboo S, Diedrich JK, Cai E, Yates JR 3rd, Ge X.

(12) Dissection of Gαs and Hedgehog signaling crosstalk reveals therapeutic opportunities to target adenosine receptor 2b in Hedgehog-dependent tumorsbioRxivFebruary 28, 2025Sarah Krantz, Braden Bell, Katherine Lund, Natalia Salinas Salinas Parra, Yeap Ng, Natalia De Oliveira Rosa, Saikat Mukhopadhyay, Brad St St Croix, Kavita Y Sarin, Roberto Weigert, et al.GAPDH (1:2000, Cell Signaling 5174), HA Tag (1:1000, Cell Signaling 3724), Gli3 (1:300, R&D Systems AF3690), SMO (1:50, Santa Cruz sc-166685), E-Cadherin (1:1000, Cell Signaling 3195), Keratin 17 (1:2000, Cell

(13) Use of patient-derived cell models for characterization of compound heterozygous hypomorphic C2CD3 variants in a patient with isolated nephronophthisisHuman Molecular GeneticsFebruary 8, 2025Zachary T Sentell, Lina Mougharbel, Zachary W Nurcombe, Sima Babayeva, Marc Henein, Lee Lee Chu, Murielle M Akpa, Chen-Fang Chung, Jean-Baptiste Rivière, Mihaela Pupavac, et al.Invitrogen, PA5–72860), Anti-⍺-tubulin (1:1000, Abcam; ab4074) and anti-GLI3 (1:1250, R&D Systems, AF3690). HRP-labeled secondary antibodies: HRP anti-goat, (1:1000, R&D; HAF109); HRP anti-mouse (1:2000, Life

(14) Non-canonical CDK6 activity promotes cilia disassembly by suppressing axoneme polyglutamylationJournal of Cell BiologyFebruary 3, 2025Kai He, Xiaobo Sun, Chuan Chen, San Luc, Jielu Hao Robichaud, Yingyi Zhang, Yan Huang, Biyun Ji, Pei-I Ku, Radhika Subramanian, et al.1:1,000; AdipoGen Life Science), Detyrosinated Tubulin (AB3201, dilution 1:5,000; Sigma-Aldrich), GLI-3 (AF3690, dilution 1:500; R&D Systems), RAB11FIP5 (14594-1-AP, dilution 1:1,000; Proteintech), CDK6 (PA5-79027,

(15) Reduction of elevated Gli3 does not alter the progression of autosomal recessive polycystic kidney diseasePhysiol RepJanuary 16, 2025Lauren G. Russell, Maria Kolatsi-Joannou, Laura Wilson, Jennifer C. Chandler, Nuria Perretta Tejedor, Georgie Stagg, Karen L. Price, Christopher J. Rowan, Tessa Crompton, Norman D. Rosenblum, et al.and incubated in the following antibodies overnight at 4°C: Goat anti‐GLI3 (1 μg/mL, R&D Systems, no. AF3690) or Rabbit anti‐alpha‐tubulin (1 μg/mL, Abcam, ab4074). Membranes were then incubated in the appropriate

(16) The Hira complex regulates Gli3R-dependent transcription in Hedgehog signaling and medulloblastoma cell growth and migrationSci RepJanuary 2, 2025Weronika Skarżyńska, Brygida Baran, Paweł Niewiadomski2643 S)",WB 1:1000 "Anti-Gli2 (R&D System, AF3635-SP)",WB 1:1000IF 1:500 "Anti-Gli3 (R&D System, AF3690)",WB 1:500IF 1:300 "Anti-SufU (Cell Signaling, C81H7)",WB 1:1000 "Anti-Arl13b (Proteintech, 17711-1-AP)",IF

(17) Intervertebral disc-intrinsic Hedgehog signaling maintains disc cell phenotypes and prevents disc degeneration through both cell autonomous and non-autonomous mechanismsCellular and Molecular Life SciencesDecember 1, 2024Lei Zhang, Siyuan Hu, Chunmei Xiu, Meng Li, Yixin Zheng, Rui Zhang, Bin Li, Jianquan ChenProteintech), goat primary antibodies against Gli2 (1:3000, #AF3635, R&D System) or Gli3 (1:2000, #AF3690, R&D System), or rabbit primary antibody against β-actin (1:2000, #4970S, Cell Signaling Technology)

(18) PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathwayCellular signallingNovember 1, 2024Baran B, Derua R, Janssens V, Niewiadomski P.

(19) CPLANE protein INTU regulates growth and patterning of the mouse lungs through cilia-dependent Hh signalingDevelopmental BiologyNovember 1, 2024Huiqing Zeng, Shimaa Ali, Aswathy Sebastian, Adriana Sophia Ramos-Medero, Istvan Albert, Charlotte Dean, Aimin Liuanti-CDH1,Sigma-Aldrich,U3254 Goat polyclonal anti-GLI2,R&D System,AF3635 Goat polyclonal anti-GLI3,R&D System,AF3690 Mouse monoclonal anti-Acta2,Sigma-Aldrich,A2547 Mouse monoclonal anti-β-tubulin,Sigma-Aldrich,T4026 Bacterial

(20) The Proteasome and Cul3-Dependent Protein Ubiquitination Is Required for Gli Protein-Mediated Activation of Gene Expression in the Hedgehog PathwayCellsSeptember 6, 2024Uśpieński T, Niewiadomski P."Cell Signaling Technologies (Danvers, MA, USA)",2643,WB (1:1000) Anti-Gli3,"R&D Systems (Minnneapolis, MN, USA)",AF3690,WB (1:1000) Anti-HA,"BioLegend (San Diego, CA, USA)",,"IF, WB (1:1000)" Anti-PARP,"Cell Signaling Technologies

Write a Review

(1)

1/21/2016Gli3 Antibody for Western Blotting Ganesh PusapatiThe goal of this antibody was to test if Gli3 full length and repressor forms are expressed in NIH3T3 cells and if their levels change in response to Sonic Hedgehog ligand addition.

Human/Mouse GLI-3 Antibody

GLI-3 Antibody

GLI-3 Antibody

GLI-3 Antibody

GLI-3 Antibody

GLI-3 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (126)

Reviews

GLI-3 Antibody from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map