Fig 2: PAF1C is a UV-induced interactor of CSB.a Results of SILAC-based MS after GFP-CSB pull-down from CS1AN-SV cells. The number of peptides identified and the UV-induced enrichment (ratio H/L) are shown. b Volcano plot depicting the UV-specific enrichment of proteins after pull-down of GFP-CSB from U2OS CSB-KO cells analyzed by label-free MS. The enrichment (log2) is plotted on the x-axis and the significance (two-sided t-test −log10 p-value) is plotted on the y-axis. Highlighted are significantly enriched subunits of RNAPII (blue) and PAF1C (red). c Co-immunoprecipitation of GFP-CSB from U2OS CSB-KO cells. d Co-immunoprecipitation of GFP or GFP-CSB from CS1AN-SV cells. e Volcano plot (as in b) depicting the enrichment of proteins or f co-immunoprecipitation after pull-down of GFP-LEO1 from RPE1-hTERT cells. g Volcano plot (as in b) depicting the enrichment of proteins or h co-immunoprecipitation after pull-down of GFP-RPB1 from U2OS cells. c, d, f, h All co-immunoprecipitation figures are representative examples of at least three independent experiments.