Fig 1: Immunofluorescence staining for detection of ZIKV antigen in mouse testes. (A) Distribution of ZIKV antigens in testis at different time points as indicated. (B) The co-localization of ZIKV antigens with cells in ZIKV-infected testes at 5 dpi (anti-ZIKV polyclonal antibodies, in green), various cell markers in testis (in red, F4/80 for macrophages, HSD3B1 for Leydig cells, α-SMA for peritubular myoid cells, FasL for sertoli cells, DDX4 for germ cells), and staining for nuclei (DAPI, in blue).
Fig 2: Arrangement of testicular cells in mice 3D testicular organoids. A The morphological structure of testicular organoids at 0–7 days post culture. Scale bar = 25 μm. B–D Distribution of spermatogenic cells marker DDX4 and Sertoli cells marker Vimentin (B), myoid epithelial cells marker α-SMA (C) or Leydig cells marker HSD3B1 (D) in shaped testicular organoids at 5th and 7th day post culture. Nuclei were visualized with DAPI. Scale bar, 5 μm. E H&E staining of the testis from 6 to 8 weeks old BALB/c male mice (ST: seminiferous tubule, Spg: spermatogonia, Spc: spermatocytes; Ser: Sertoli cell; Myo: myoid epithelial cell). Scale bar, 50 μm.
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