Fig 1: Radiation dose-response of HSPCs and osteoblastic differentiation in mouse BM cells. (A) BM cells were irradiated with α-radiation or X-irradiation and dose-response curves of total CFCs were plotted. Representative images of colony-forming cells (B) CFU-GEMM, (C) BFU-E and (D) CFU-G/GM from BM cultures are shown. (E) Quantification of each colony type is presented. (F-J) Flow cytometric analysis of BM cultures was performed. Quantitative values (F-H) and representative histograms (I-K) are shown for (F and I) CD45 expression and osteoblastic differentiation markers (G and J) RUNX2 and (H and K) BAP. Each value represents the mean ± SD of 8-10 mice per group. Statistical analysis was performed using one-way ANOVA followed by Tukey-Kramer's multiple comparisons test. *P<0.05, **P<0.01 vs. undifferentiated BM-MNCs. HSPCs, hematopoietic stem/progenitor cells; BM, bone marrow; CFCs, colony-forming cells; CFU-GEMM, colony-forming unit-granulocyte, erythroid, macrophage and megakaryocyte; BFU-E, burst-forming unit-erythroid; CFU-G/GM, colony-forming unit-granulocyte and macrophage; MNCs, mononuclear cells ; NC, negative control.