Brilliant Violet 421™ anti-mouse CD4 100563 from BioLegend

Product Specs
ItemBrilliant Violet 421™ anti-mouse CD4
CompanyBioLegend
Price $274.00Supplier Page View Company Product Page
Catalog Number100563
Quantity50UG
ApplicationsFC,ICC,IHC-F
Concentration0.2000mg/mL
Conjugate/TagBrilliant Violet 421™
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ReactivityMouse
IsotypeRat IgG2a, κ
CloneRM4-5
HostRat
Antibody TypePrimary
ImmunogenBALB/c mouse thymocytes
NCBI Full Gene NameCD4 antigen
NCBI Gene AliasesL3T4, Ly-4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Regulatory B cells contribute to allergen-encapsulating nanoparticle immunotherapy efficacy for food allergyJCI InsightAugust 10, 2026Laila M. Rad, Michael N. Saunders, Laura A. Williams, Katarzyna W. Janczak, Chris L. Dorsett, Kate V. Griffin, Elizabeth J. Bealer, Jeffrey A. Ma, Sayre A. Tillery, Jyotirmoy Roy, et al.Live/Dead Fixable Violet (Thermo Fisher Scientific, L34964). Panel 2 comprises CD4 (BV421; BioLegend, 100543), CD8 (FITC; BioLegend, 100705), B220 (PerCP; BioLegend, 103234), CD138 (PE/Cy7; BioLegend, 142514),

(2) Mucosal vaccination with enzymatically active Helicobacter pylori γ-glutamyl transferase (GGT) adjuvanted with STING-agonist elicits robust Th1/Th17 immunity in miceFrontiers in MicrobiologyJune 30, 2026Monish Kumar, Jiayan Li, Navin Varadarajanthen stained for extracellular markers CD3-BUV395 (BD Biosciences #569614), CD4-BV421 (Biolegend, #100543) and CD8-AF488 (Biolegend, #100726) at 4 °C for 1 h. The cells were then fixed for 20 min at 4 °C using

(3) Deficiency of lysosomal TMEM175 in myeloid macrophages exerts anti-tumor immunity via inflammasome and cross-presentation pathwayNature CommunicationsFebruary 14, 2026Ziqi Zhang, Xue li, Tianqi Lu, Jingyun Yang, Xuejiao Han, Zhenfei Bi, Jingwen Luo, Aqu Alu, Weiqi Hong, Siyuan Chen, et al.(BioLegend, 100516), PerCP-Cy5.5 anti-mouse CD4 (BD Biosciences, 550954), BV421 anti-mouse CD4 (BioLegend, 100543), BV650 anti-mouse NK1.1 (BioLegend, 108735), APC anti-mouse NK1.1 (BioLegend, 108710), BV421 anti-mouse

(4) Bioinformatic analysis, clinical implications and experimental validation of ferroptosis-related feature gene in IgA nephropathy: focus on DUSP1Front Med (Lausanne)August 7, 2025Tingting Liu, Tingting Pan, Mingxin Chang, Shaojie Fu, Hongzhao Xu, Hao Wu, Zhonggao Xu, Yanli ChengSubsequently, fluorochrome-conjugated antibodies against CD3 (100203, BioLegend, United States) and CD4 (100543, BioLegend, United States) were added, and the cells were incubated in the dark at 4°C for 30 min. Following

(5) KMT2D coordinates antiviral CD4+ T cell responses through opposing effects on T follicular helper and cytotoxic gene expressionCell ReportsJune 24, 2025Jonathan A. Cohen, Ashlyn A. Buzzelli, Gabrielle Quickstad, Lixin Yang, Ichiro Misumi, Karl B. Shpargel, Jason K. WhitmireRRID:AB_2922449 anti-mouse-CD4-SparkPlusUV395,Biolegend,Cat #100495; RRID:AB_3097649 anti-mouse-CD4-BV421,Biolegend,Cat #100563; RRID:AB_2563052 anti-mouse-CD4-Violet Fluor 500,Cytek,Cat #850041U025 anti-mouse-CD4-FITC,Biolegend,Cat

(6) Impact of Extended Dosing Intervals and Ipsilateral Versus Contralateral Boosting on mRNA Vaccine Immunogenicity in MiceVaccines (Basel)March 1, 2025Bin Lu, Omkar Chaudhary, Balaji Banoth, Janhavi Nadkarni, Wei Zong, Emilie Mausser, Hillary Danz, Mona Motwani, Sophie Ruiz, Donghui Zhang, et al.Franklin Lakes, NJ, USA] CD3-BV605 (cat#: 564009); [BioLegend, San Diego, CA, USA] CD4-BV421 (cat#: 100543), CXCR5-BV650 (cat#: 145517), PD1-PE (cat#: 109104GC B cells: [BD Biosciences, Franklin Lakes, NJ, USA]

(7) A yeast-based oral therapeutic delivers immune checkpoint inhibitors to reduce intestinal tumor burdenCell Chemical BiologyJanuary 16, 2025Olivia N. Rebeck, Miranda J. Wallace, Jerome Prusa, Jie Ning, Esse M. Evbuomwan, Sunaina Rengarajan, LeMoyne Habimana-Griffin, Suryang Kwak, David Zahrah, Jason Tung, et al.APC/Cyanine7 anti-mouse CD4,BioLegend,Cat#100526 (RRID: AB_312727) Brilliant Violet 421™ anti-mouse CD4,BioLegend,Cat#100563 (RRID: AB_2563052) PerCP/Cyanine5.5 anti-mouse CD8a,BioLegend,Cat#100734 (RRID: AB_2075238) Brilliant Violet 711™ anti-mouse/human CD44

(8) Elucidating Sex-Specific Immune Profiles in a Breast Cancer ModelInternational Journal of Molecular SciencesDecember 6, 2024Ebony Hargrove-Wiley, Dora Obodo, Wendy Bindeman, Barbara FingletonCA, USA): CD45-APC/Cy7 (Cat. No. 103116), CD3-PerCP/Cy5.5 (Cat. No. 100328), CD4-Br.Viol421 (Cat. No. 100543), CD8-APC (Cat. No. 100712), NK1.1-PE (Cat. No. 108708), CD11b-Alexa488 (Cat. No. 101217), CD19-PerCP/Cy5.5

(9) A hypoxia-activated and microenvironment-remodeling nanoplatform for multifunctional imaging and potentiated immunotherapy of cancerNature CommunicationsNovember 29, 2024Jianwen Song, He Wang, Xue Meng, Wen Li, Ji Qianti-CD86-APC (Biolegend, 105011, 1:200); anti-CD3-FITC (Biolegend, 100203, 1:200); anti-CD4-BV421 (Biolegend, 100543, 1:200); anti-CD8a-BV421 (Biolegend, 100711, 1:200); anti-Foxp3-PE (Biolegend, 320007, 1:200); anti-F4/80-PE

(10) An IL-2 mutein increases regulatory T cell suppression of dendritic cells via IL-10 and CTLA-4 to promote T cell anergyCell ReportsNovember 26, 2024Braxton L. Jamison, Matthew Lawrance, Chun Jing Wang, Hannah A. DeBerg, Lauren J. Ziegler, David M. Sansom, Marc A. Gavin, Lucy S.K. Walker, Daniel J. CampbellBV650 (RM4-5),BioLegend,Cat# 100555; RRID: AB_2562529 Anti-mouse CD4 BV421 (RM4-5),BioLegend,Cat# 100563; RRID:AB_2563052 Anti-mouse CD45 BUV395 (30-F11),BD Biosciences,Cat# 564279; RRID:AB_2651134 Anti-mouse

(11) Muscle inflammation is regulated by NF-κB from multiple cells to control distinct states of wasting in cancer cachexiaCell ReportsNovember 26, 2024Benjamin R. Pryce, Alexander Oles, Erin E. Talbert, Martin J. Romeo, Silvia Vaena, Sudarshana Sharma, Victoria Spadafora, Lauren Tolliver, David A. Mahvi, Katherine A. Morgan, et al.107651; RRID:AB_2616728 Siglec F,Thermo Fisher,Cat# 367–1702-80; RRID:AB_2895998 Ly-6C,Biolegend,Cat# 100543; RRID:AB_10898318 CD8a,Biolegend,Cat# 100741; RRID:AB_11124344 CD11b,BD Bioscience,Cat# 564454; RRID:AB_2665392 CD161,Biolegend,Cat#

(12) Inhibitory co-receptor Lag3 supports Foxp3+ regulatory T cell function by restraining Myc-dependent metabolic programmingImmunityNovember 1, 2024Dongkyun Kim, Giha Kim, Rongzhen Yu, Juyeun Lee, Sohee Kim, Mia R. Gleason, Kevin Qiu, Elena Montauti, Li Lily Wang, Deyu Fang, et al.RM4-5),Biolegend,Cat # 100528;RRID:AB_312729 "anti-mouse CD4, BV421 (clone RM4-5)",Biolegend,Cat # 100563;RRID:AB_2563052 "anti-mouse CD8, APC (clone 53-6.7)",BD Biosciences,Cat #553035;RRID:AB_398527 "anti-mouse

(13) Sex-dependent APOE4 neutrophil-microglia interactions drive cognitive impairment in Alzheimer's diseaseNature medicineOctober 1, 2024Rosenzweig N, Kleemann KL, Rust T, Carpenter M, Grucci M, Aronchik M, Brouwer N, Valenbreder I, Cooper-Hohn J, Iyer M, et al.were washed, pelleted and stained with the following rat antibodies: BV421 anti-CD4 (1:100, Biolegend, 100543), FITC anti-CD3 (1:100, Biolegend, 100203), PE anti-CD8 (1:100, Biolegend, 100707) and APC anti-TCRγδ

(14) Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass SpectrometryCancer immunology researchAugust 1, 2024Meng W, Takeuchi Y, Ward JP, Sultan H, Arthur CD, Mardis ER, Artyomov MN, Lichti CF, Schreiber RD.BioLegend, Cat# 126609), BV711 anti-CD4 (RM4–5, BioLegend, Cat# 100549), BV421 (RM4–5, BioLegend, Cat# 100543), PECy7 anti-CD90.2 (30-H12, BioLegend, Cat# 105325), FITC anti-CD11b (M1/70, BioLegend, Cat# 101205),

(15) Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass SpectrometryCancer Immunology ResearchAugust 1, 2024Wei Meng, Yoshiko Takeuchi, Jeffrey P. Ward, Hussein Sultan, Cora D. Arthur, Elaine R. Mardis, Maxim N. Artyomov, Cheryl F. Lichti, Robert D. SchreiberBioLegend, Cat# 126609), BV711 anti-CD4 (RM4–5, BioLegend, Cat# 100549), BV421 (RM4–5, BioLegend, Cat# 100543), PECy7 anti-CD90.2 (30-H12, BioLegend, Cat# 105325), FITC anti-CD11b (M1/70, BioLegend, Cat# 101205),

(16) Periodontitis exacerbates pulmonary hypertension by promoting IFNγ(+) T cell infiltration in miceInternational journal of oral scienceMarch 28, 2024Meng X, Du L, Xu S, Zhou L, Chen B, Li Y, Chen C, Ye H, Zhang J, Tian G, et al.h. Antibodies against CD45-BV510 (103137, Biolegend), CD3-FITC (553061, BD Pharmingen), CD4-BV421 (100543, Biolegend), CD8-PE-Cy7 (100721, Biolegend), IL-17A-PE (506903, Biolegend) and IFN-γ-APC (505809, Biolegend)

(17) Conserved molecular chaperone PrsA stimulates protective immunity against group A Streptococcusnpj VaccinesFebruary 26, 2024Chien-Yu Lai, Jia-Xun Xie, Meng-Chih Lai, Zhao-Yi Wu, Jr-Shiuan Lin, Yu-Tsung Huang, Chia-Yu Chi, Chuan Chiang-Ni, Mark J. Walker, Yung-Chi Changanti-mouse CD3-APC/Cy7 antibody (BioLegend, Cat. No. 100329), CD4-BV421 antibody (BioLegend, Cat. No. 100543), and CD8-PE Dazzle 594 antibody (BioLegend, Cat. No. 100761). Following fixation with 4% paraformaldehyde

(18) Long-lived autoreactive memory CD4(+) T cells mediate the sustained retinopathy in chronic autoimmune uveitisFASEB journal : official publication of the Federation of American Societies for Experimental BiologyApril 1, 2023Fan NW, Zhu Q, Wang S, Ortiz G, Huckfeldt RM, Chen Y.

(19) The altering cellular components and function in tumor microenvironment during remissive and relapsed stages of anti-CD19 CAR T-cell treated lymphoma miceFrontiers in immunologyJanuary 1, 2023Zhao K, Ren C, Tang D, Zhao L, Chen X, Wang Y, Xu K.

(20) Glucose promotes regulatory T cell differentiation to maintain intestinal homeostasisiScienceSeptember 16, 2022Yu Y, Yang W, Yu T, Zhao X, Zhou Z, Yu Y, Xiong L, Yang H, Bilotta AJ, Yao S, et al.

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11/15/2016Brilliant Violet Antibody for CD4 Mithun KhattarCD4 is a very common marker in lymphocyte staining panels. Since its a lineage marker, I wanted to try staining it using uncommon fluorophores, especially on the brilliant violet laser. Among all the ... Read Review

Brilliant Violet 421™ anti-mouse CD4 from BioLegend

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Brilliant Violet 421™ anti-mouse CD4 from BioLegend

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