Brilliant Violet 570™ anti-mouse CD4 100542 from BioLegend

Product Specs
ItemBrilliant Violet 570™ anti-mouse CD4
CompanyBioLegend
Price $275.00Supplier Page View Company Product Page
Catalog Number100542
Quantity50 µg
ApplicationsFC - Quality tested
Concentrationµg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Conjugate/TagBrilliant Violet 570™
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ReactivityMouse
IsotypeRat IgG2a, kappa
CloneRM4-5
HostRat
Antibody TypePrimary
ImmunogenBALB/c mouse thymocytes
NCBI Full Gene NameCD4 antigen
NCBI Gene AliasesL3T4, Ly-4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Intramuscular plasmid DNA electroporation sequesters neoantigen-specific CD8+ T cells in treated muscle and limits tumor infiltrationMolecular Therapy OncologySeptember 17, 2026Bianchi Andrea, Esposito Mauro, Giacomelli Tiziano, Esposito Ilaria, Tonini Claudia, Aurisicchio Luigi, Palombo Fabio46-0032-80) (1:160), CD4- PerCP-eFluor710 (Invitrogen cat.46-0042-82) (1:200) or BV570 (BioLegend, cat. 100541) (1:200), CD8-APC-eFluor780 (Invitrogen cat.470081-82) (1:200) or SB702 (Invitrogen, cat. 67-0081-82)

(2) The initial melanoma T cell infiltrate is defined by tissue-resident programs restrained by regulatory T cellsResearchSquareJuly 16, 2026Jason B. Williams, Shishir M. Williams, Alexander L. Pant, Alexander Rajmalani, Clarence Rajmalani, Clarence Zhang, Jiang Zhang, Elizabeth Rotrosen, Elizabeth Rotrosen, Angelika Stoecklinger, et al.532,M1/69,ebioscience,58-0242-82 CD45 APC-Fire 810,30-F11,BioLegend,103174 XCR1 BV785,ZET,BioLegend,148225 CD4 BV570,RM4–5,BioLegend,100541 MHCII,M5/114.15.2,eBiosciences,48-5321-82 Thy1.1 BV711,OX-7,BioLegend,202539 Thy1.1 PE-Cy7,OX-7,BioLegend,202518 Thy1.2

(3) Hypoxia shapes both therapeutic response and resistance in metastatic clear cell renal cell carcinomaCancer CellJuly 13, 2026Lynda Vuong, Andrew E. Cornish, Jennifer Pfeil, Yash Khandwala, Hui Jiang, Doris X.T. Zheng, Eduardo A. Mascareno, Nicole Rittenhouse, Phillip M. Rappold, Josef Leibold, et al.BE0003-1 anti-mouse CD4-BUV395 (GK1.5),BD Biosciences,Cat # 563790 Anti-mouse CD4-BV570 (RM4-5),Biolegend,Cat # 100541 anti-mouse PD-1-BUV615 (RMP1-30),BD Biosciences,Cat # 752354 anti-mouse PD-1-BUV615 (29F 1A12),BD Biosciences

(4) BPTF is essential for vaccine-induced germinal center B cell responsesJ ImmunolJune 20, 2026Alexandria J Sturtz, Birk K Evavold, Zarifeh Heidari Rarani, Wafaa B Alsoussi, Julian Q Zhou, Hanover C Matz, Hiromi Muramatsu, Wren Simkins, Sameer Kumar Malladi, Katherine M McIntire, et al.Cat#62-0711-82), IgG1-BV510 (1:100, RMG1-1; BioLegend; Cat#406621), CD4-BV570 (1:200, RM4-5; BioLegend; Cat#100542), CXCR4-BV605 (1:50, L276F12; BioLegend; Cat#146519), IgD-BV711 (1:200, 11-26c.2a; BioLegend; Cat#405731),

(5) Mucosal vaccination clears Clostridioides difficile colonizationNatureApril 1, 2026Thomas AK, Peritore-Galve FC, Ehni AG, Lança BBC, Coggin J, Brady EJ, Yoder SM, Shrem R, Rodríguez RC, Kroh HK, et al.109238), anti-B220 (FITC, RA3-6B2, 1:600, BioLegend, 103205), anti-CD4 (BV570, RM4-5, 1:150, BioLegend, 100541), anti-CD8 (Alexa Fluor 532, 53-6.7, 1:300, Thermo Fisher Scientific, 58-0081-80), anti-CD69 (APC, H1.2F3,

(6) Exogenous sphingomyelinase mediates MSC-derived EV biogenesis and enhances potency via repackaging of molecular cargoiScienceMarch 20, 2026Daniel C. Shah, S'Dravious A. DeVeaux, Hongmanlin Zhang, Alan Y. Liu, Tosin A. Adedipe, Nathan F. Chiappa, Afra I. Toma, Krishna A. Patel, Young C. Jang, Steven L. Goudy, et al.,BD Biosciences,751213; RRID: AB_2875235 CD3 Pacific Blue™,BioLegend,100213; RRID: AB_493644 CD4 BV570,BioLegend,100541; RRID: AB_10897943 CD8a Alexa Flour™ 700,BioLegend,100729; RRID: AB_493702 EpCAM/CD326,BD Biosciences,563134;

(7) Regulatory T cells inhibit CD8 + T RM -like cells during the early stages of tumor immune escapebioRxivMarch 6, 2026Jason B. Williams, Shishir M. Pant, Alexander L. Kley, Bharat A. Rajmalani, Clarence Yapp, Jiang Zhang, Kyle Deans, Elizabeth Rotrosen, Angelika Sales, Peter K. Soger, et al.532,M1/69,ebioscience,58-0242-82 CD45 APC-Fire 810,30-F11,BioLegend,103174 XCR1 BV785,ZET,BioLegend,148225 CD4 BV570,RM4-5,BioLegend,100541 MHCII,M5/114.15.2,eBiosciences,48-5321-82 Thy1.1 BV711,OX-7,BioLegend,202539 Thy1.1 PE-Cy7,OX-7,BioLegend,202518 Thy1.2

(8) Unconventional CD8+ T cell surveillance of cytomegalovirus via Qa-1/HLA-E-restricted epitope recognitionSci AdvDecember 19, 2025Shanelle P. Reilly, Madison L. Smith, Samantha M. Borys, Céline Fugère, Delia Demers, Michael J. Hogan, David Zemmour, Laurent BrossayBUV395 Hamster Anti-Mouse CD3e (BD Biosciences no. 563565, RRID: AB_2738278), BV570 Rat Anti-Mouse CD4 (BioLegend no. 100542, RRID:AB_2563051), BV650 Rat Anti-Mouse CD4 (BioLegend no. 100469, RRID: AB_2783035), APC-eFluor780

(9) Topoisomerase III-beta protects from immune dysregulation and tumorigenesisSci AdvDecember 19, 2025Md Rasel Al Mahmud, Simone Andrea Baechler, Anjali Dhall, Sourav Saha, Hongliang Zhang, Laura Bassel, Shuling Zhang, Min-Jung Lee, Nahoko Sato, Shraddha Rastogi, et al.antibodies for surface staining for 20 min at 4°C. The antibodies used were CD4 (RM4-5, BioLegend, #100542), CD8 (53-6.7, BioLegend, #100733), CD3 (17A2, BioLegend, #100237), CD11b (1D3/39, BioLegend, #152408),

(10) Pro-Fibrotic Immune Cells and Fibro-Adipogenic Progenitors Contribute to Skeletal Muscle Extracellular Matrix Remodeling and Fibrosis in Cancer CachexiaAmerican Journal of Physiology-Cell PhysiologyOctober 1, 2025Thomas D. Cardaci, Arianna V. Bastian, Kasie Roark, Brooke M. Bullard, Mitchell M. NeSmith, Christian A. Unger, Robert L. Price, Jason L. Kubinak, E. Angela Murphy, Brandon N. VanderVeenNumber AF488,Ter119,BioLegend,116215 PerCP/Cy5.5,CD8a,BioLegend,100734 APC,CD106,BioLegend,105718 AF700,CD31,BioLegend,102443 APC/Fire 750,Ly6c,BioLegend,128045 BV421,CD11c,BioLegend,117329 BV570,CD4,BioLegend,100541 BV605,MHCII,BioLegend,107639 BV650,CCR2,BioLegend,150613 BV711,CD68,BioLegend,137029 BV750,CD11b,BioLegend,101267 BV785,c-Kit,BioLegend,105841 PE,NK1.1,BioLegend,108707 PE/Dazzle

(11) Early-life thymectomy results in visceral adipose tissue inflammation and glucose intoleranceImmun AgeingOctober 1, 2025David J. Buckley, Sogol Zahedi, Prema Velusamy, Sunita Sharma, Manoj Sabnani, Lisa C. Flores, Ganga Tandukar, Yuji Ikeno, Paul J. Fadel, Lisa A. Lesniewski, et al.Invitrogen#59–0454-82 (total leukocytes), violetfluor500 CD3, Tonbo#85–0032 (pan T cells), BV570-CD4, Biolegend#100542 (T helper cells), PerCP-Cy5.5-CD8, Tonbo#65–0081 (cytotoxic T cells), SB645 CD44, Invitrogen#64–0441-82

(12) NNMT inhibition in cancer-associated fibroblasts restores antitumour immunityNatureSeptember 25, 2025Janna Heide, Agnes J. Bilecz, Samarjit Patnaik, Maria Francesca Allega, Leonhard Donle, Kaiting Yang, Ethan Teich, Yan Li, Qiaoshan Lin, Ke Kong, et al.(Biolegend, 126419, 1:200), CD11b (Biolegend, 101224, 1:200), CD19 (BD, 566167, 1:200), CD4 (Biolegend, 100542, 1:200 or 100512, 1:200), PD-L1 (Biolegend, 124336, 1:200), CD3 (Biolegend, 100249, 1:200 or 100216,

(13) Multi-omic analysis identifies erythroid cells as the major population in mouse placentas expressing genes for antigen presentation in MHC class II, chemokines, and antibacterial immune responseFrontiers in ImmunologySeptember 22, 2025Olga Perik-Zavodskaia, Roman Perik-Zavodskii, Saleh Alrhmoun, Kirill Nazarov, Julia Shevchenko, Konstantin Zaitsev, Sergey SennikovPacific Blue anti-mouse TER - 119/erythroid cells (Cat # 116232), and Brilliant Violet 570 anti-mouse CD4 (Cat # 100542) (BioLegend, USA). After incubating the cells in the dark for 30 – 40 minutes, we washed them with 0.5

(14) Anti-vascular endothelial growth factor treatment potentiates immune checkpoint blockade through a BAFF- and IL-12-dependent reprogramming of the TMEImmunityApril 8, 2025Mohamed-Reda Benmebarek, Cihan Oguz, Matthias Seifert, Benjamin Ruf, Yuta Myojin, Kylynda C. Bauer, Patrick Huang, Chi Ma, Marina Villamor-Payà, Francisco Rodriguez-Matos, et al.RRID:AB_2562358), anti-CD103-BV510 (Biolegend Cat. No 121423, RRID:AB_2562713), anti-CD4-BV570 (Biolegend Cat. No 100542, RRID:AB_2563051), anti-T-bet-BV650 (BD Cat. No 564142, RRID:AB_2738616), anti-CCR8-BV711 (Biolegend

(15) Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosisCell ReportsMarch 25, 2025Samantha M. Golomb, Ian H. Guldner, Emilija Aleksandrovic, Shaneann R. Fross, Xiyu Liu, Lu Diao, Karena Liang, Jinxuan Wu, Qingfei Wang, Jacqueline A. Lopez, et al.53–6.7), rat anti-mouse CD3-PacificBlue (BioLegend, 100213, 17A2), rat anti-mouse CD4–570 (BioLegend, 100541, RM4–5), rat anti-mouse CD8a-APC-Cy7 (BioLegend, 100713, 53–6.7), hamster anti-mouse TCR γ/δ−711 (BioLegend,

(16) Peptide-Drug Conjugate for Therapeutic Reprogramming of Tumor-Associated Macrophages in Breast CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2025Lepland A, Peranzoni E, Haljasorg U, Asciutto EK, Crespí-Amer M, Modesti L, Kilk K, Lombardia M, Acosta G, Royo M, et al.M024T02B06,Thermofisher F4/80,SBB765,Cl:A3‐1,MCA497SBB765,Bio‐Rad CD45,SBB810,YW62.3,MCA1031SBB810,Bio‐Rad CD206,PE‐Fire700,C068C2,141742,Biolegend PDL1,NovaFluorRed700,MIH5,M036T03R03,Thermofisher CD11b,APC‐Cy7,N418,117324,Biolegend B220,APC‐Fire810,RA3‐6B2,103278,Biolegend GRZB,BV421,QA18A28,396414,Biolegend CD49,PacBlue,DX5,108918,Biolegend CD4,BV570,RM4‐5,100542,Biolegend CD27,BV605,LG 3A10,124249,Biolegend CD8,BV650,53‐6.7,100742,Biolegend CD44,BV711,IM7,103057,Biolegend FOXP3,AF488,150D,320012,Biolegend CD3,SparkBlue‐574,17A2,100276,Biolegend CD19,SBB765,6D5,MCA1439SBB765,Bio‐Rad IL7RA,PE‐Cy7,A7R34,135014,Biolegend CD25,APC,PC61,102012,Biolegend KI67,AF700,16A8,652420,Biolegend CD62L,APC‐Cy7,MEL‐14,104428,Biolegend Antibodies

(17) Type I IFN-mediated NET release promotes Mycobacterium tuberculosis replication and is associated with granuloma caseationCell Host & MicrobeDecember 11, 2024Chanchal Sur Chowdhury, Rachel L. Kinsella, Michael E. McNehlan, Sumanta K. Naik, Daniel S. Lane, Priyanka Talukdar, Asya Smirnov, Neha Dubey, Ananda N. Rankin, Samuel R. McKee, et al.HK1.4),BioLegend,Cat# 128036 BV570™ anti-CD4 rat monoclonal antibody (clone RM4-5),BioLegend,Cat# 100542 BV421™ anti-TCRp Armenian hamster IgG (clone H57-597),BioLegend,Cat# 109230 APC-Fire750 anti-CD8a

(18) Dietary restriction impacts health and lifespan of genetically diverse miceNatureOctober 17, 2024Andrea Di Francesco, Andrew G. Deighan, Lev Litichevskiy, Zhenghao Chen, Alison Luciano, Laura Robinson, Gaven Garland, Hannah Donato, Matthew Vincent, Will Schott, et al.(25-0441-U100, Tonbo Biosciences, 1:40); Ly6G BV421, 1A8, (562737, BD Biosciences, 1:80); CD4 BV570, RM4-5 (100542, BioLegend, 1:40); CD11b BV650, M1/70 (563402, BD Biosciences, 1:160); CD45R/B220 BUV496 (RA3-6B2, 564662,

(19) Protocol to study the immune profile of syngeneic mouse tumor modelsSTAR protocolsSeptember 20, 2024Miyauchi S, Arimoto KI, Liu M, Zhang Y, Zhang DE.109822" CD3,17A2,BV711,1:100,"BioLegend, Cat #: 100241" CD4,RM4-5,BV570,1:500,"BioLegend, Cat #: 100542" CD8a,53–6.7,PE-Cy5,1:200,"BioLegend, Cat #: 100710" CD25,PC61,PE,1:100,"BioLegend, Cat #: 102008" CD62L,MEL-14,PE-Cy7,1:100,"BioLegend,

(20) Transcriptomic analysis unveils bona fide molecular signatures of microglia under conditions of homeostasis and viral encephalitisJournal of NeuroinflammationAugust 17, 2024Felix Mulenge, Olivia Luise Gern, Lena Mareike Busker, Angela Aringo, Luca Ghita, Inken Waltl, Andreas Pavlou, Ulrich Kalinke(Biolegend, 149025), MHCI BUV661 (BD, 749702), MHCII BV785 (Biolegend, 107645), CD4 BV570 (Biolegend, 100541), CD8b BV711 (Biolegend, 126633), CD11c BV605 (BD, 563057), TCR-β PE-dazzle (Biolegend, 109240), B220

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5/8/2020Great Antibody to Detect CD4 T Cells Via Flow Cytometry Chirag PatelI would recommend this antibody to anyone studying T cells. It gives a strong, consistent labeling of CD4 T cells.

Brilliant Violet 570™ anti-mouse CD4 from BioLegend

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Brilliant Violet 570™ anti-mouse CD4 from BioLegend

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