Great Antibody to Detect CD4 T Cells Via Flow Cytometry

May 08, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Brilliant Violet 570™ anti-mouse CD4 Antibody

Catalog Number:

100542

Clone Number:

Clone RM4-5

We are an immunology lab that looks at immune responses in the context of inflammation and cancer. This marker is used to widely used to identify CD4 T cells in flow cytometry experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes (Naive and Infected)

Primary Incubation

Multiple Dilutions (1:250, 500, 1000)

Blocking Agent

FC Block 100ng/mL in FACS solution (surface)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora (BVR)

Results Summary

Splenic CD4 T cells are strongly and specifically labelled to identify the population with CD4 BV570.

DOI or PMID #

N/A

Additional Notes

Accordinly Titrate Antibody based on flow analyzer.

Related Categories

Image Gallery

Summary

The Good

Reliable results as easy to idenitify CD4+ T cells from other spenic cells.

The Bad

None

The Bottom Line

I would recommend this antibody to anyone studying T cells. It gives a strong, consistent labeling of CD4 T cells.

Share your experience with other scientists. Write a Review! »

Join the discussion