Western Blot: CDC2L5 Antibody [NB100-68268] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC2L5 antibody NB100-68268 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CDC2L5 was also immunoprecipitated by rabbit anti-CDC2L5 antibody that recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes (A) and 10 seconds (B).