Mouse IgG Isotype Control 31903 from Thermo Fisher Scientific

Product Specs
ItemMouse IgG Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number31903
Quantity5 mg
ApplicationsControl, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Inhibition Assays
Conjugate/TagUnconjugated
FormatPBS with no preservative; pH 7.6
TargetMouse IgG
IsotypeIgG
HostMouse
Concentration5.05 mg/mL
Storage Temperature4° C
PurityIon-exchange chromatography
Antibody TypeIsotype Control
Storage4°C
ClonalityPolyclonal

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(1) Large-scale single-molecule analysis of tau proteoformsNat MethodsSeptember 4, 2026James Joly, Vivekananda Budamagunta, Zhengjian Zhang, Brittany Nortman, Maryam Jouzi, Rajat Bhatnagar, Jarrett D. Egertson, Michael E. Flaster, Rob Grothe, Sanjib Guha, et al.the N terminus, middle region and C terminus of tau, optionally together with mouse IgG (Invitrogen 31903) as the negative control, are immobilized individually using Dynabeads Antibody Coupling Kit (Invitrogen,

(2) Detection of Human Cytomegalovirus lncRNA4.9 in Breast Tumor Tissue Using RNA In Situ HybridizationJ Histochem CytochemAugust 19, 2026Arthur Morley-Bunker, Georgia Turner, Brooke Beardsley, Helen Morrin, John Pearson, Logan C. Walker, Margaret Currienegative phosphate-buffered saline (PBS) control, and a mouse IgG isotype control (Catalog number #31903, Invitrogen, Thermofisher, Rockford, IL). To perform antigen retrieval, slides were deparaffinized and

(3) BRAF Inhibition–Associated Nuclear Remodeling is Linked to Cancer-Associated Fibroblast ActivationCancer Res CommunJuly 1, 2026Jie Wang, Bruna da Silva Soley, Yao Xiao, Lindsey G. Siegfried, Linli Zhou, Mingang Xu, Sarah Millar, Thomas Andl, Yuhang Zhangantibody (Proteintech, cat. #60003-2-Ig, RRID: AB_2734122) or mouse IgG (Thermo Fisher Scientific, cat. #31903) was added to a spin column containing 20 μL of resin slurry in a 1.5-mL collection tube. The antibodies

(4) In Vitro Capacitation in Boar Sperm: Evaluation of Selected Detection TechniquesMethods ProtocJune 15, 2026Barbora Klusackova, Zuzana Pilsova, Katerina Nemeckova, Aneta Pilsova, Pavla Postlerovaovernight at 4 °C with anti-phosphotyrosine antibody (clone 4G10; 1:1000) or mouse IgG isotype control (#31903, ThermoFisher Scientific, Waltham, MA, USA; 1:10,000). For densitometric analysis, the membranes were

(5) Ppm1d Clonal Hematopoiesis Does Not Promote Atherosclerosis in Murine ModelsArterioscler Thromb Vasc BiolMay 21, 2026Cesar De Jeronimo Diaz, Huajun Liao, Brian Hardaway, Linke Li, Anna Lu Gan, Mandy Liu, Alice Kim, Grace Qian, Sandra Abramowicz, Edithe Lam, et al.#9718 1:200). Rabbit IgG (Invitrogen #31235), Rat IgG (Invitrogen #31933), and Mouse IgG (Invitrogen #31903) isotype controls were used as negative controls at concentrations matched to their corresponding primary

(6) Medium Composition Determines the Dynamics of Boar In Vitro Sperm Capacitation-Associated EventsInt J Mol SciMay 19, 2026Barbora Klusackova, Zuzana Pilsova, Barbora Bryndova, Aneta Pilsova, Natalie Zelenkova, Petr Pecina, Michal Knezu, Petra Secova, Pavla Tymich Hegrova, Eva Chmelikova, et al.mouse anti-phosphotyrosine antibody (clone 4G10; Merck Millipore; 1:300) or an IgG Isotype Control (#31903, Invitrogen; Waltham, MA, USA; 1:1000). After washing with PBS, samples were labeled with Alexa Fluor®

(7) Embryonic stem cell factors DPPA2/4 amplify active H3K4me3–H2AK119ub chromatin domains in non-small cell lung cancerGenes & DevelopmentMay 4, 2026Janith A. Seneviratne, Clare L. Crisp, Eleanor Glancy, Natalie Choy, Winnie Tan, Matthew Neve, Melanie Stammers, Tongtong Wang, Ruby Johnstone, Arshnoor Kaur, et al.conjugate with 80 µL of washed beads while rotating for 5 h at 4°Cusing 0.5–4 µg of mouse IgG (Invitrogen 31903) and 0.5–4 µg of rabbit IgG (Invitrogen 012-6102) as IgG controls or the following antibodies: 4 µg

(8) Restoring disc matrix homeostasis: Dual-miRNA and human platelet lysate as a novel therapeutic strategyMater Today BioMay 3, 2026Tara Ní Néill, Niamh Wilson, Jijo Thomas, Jake McDonnell, Stacey L. Darwish, Joseph S. Butler, Fergal J. O'Brien, James E. Dixon, Caroline M. Curtin, Conor T. BuckleyCode,Antigen Retrieval Method IgG,Rabbit,1:1250,"Invitrogen, 26102",HIER IgG,Mouse,1:1250,"Invitrogen, 31903",HIER Aggrecan,Rabbit,1:250,"Invitrogen, MA532695",HIER SOX9,Rabbit,1:250,"Invitrogen, PA586301",HIER Collagen

(9) Mapping the nanoscale organization of the human cell surface proteome reveals new functional associations and surface antigen clustersbioRxivApril 11, 2026Brendan M. Floyd, Elizabeth L. Schmidt, Nicholas A. Till, Jonathan L. Yang, Pinyu Liao, Benson M. George, Ryan A. Flynn, Carolyn R. Bertozzisupernatant and resuspended in 1 mL of cold DPBS containing 25 µg of isotype control (Thermo Scientific #31903) or 25 µg of the respective targeting antibody. The cells were then incubated at 4°C for 30 min on a

(10) The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanismsCell Biology and ToxicologyApril 2, 2026Hongtao Ren, Mincong Wang, Xiulong Ma, Lei An, Yuyan Guo, Hongbing MaA/G agarose and anti-m6A (1:3000, Cat. No. 68055–1-Ig, Proteintech), isotype IgG (1:3000, Cat. No. 31903, Thermo Fisher Scientific), anti-IGF2BP2 (1:1000, Cat. No. 11601–1-AP, Proteintech), or anti-METTL3

(11) Quantitative immunohistochemistry and the use of cellular calibrators for HER2 receptor number determinationScientific ReportsMarch 23, 2026Kevin McKinski, Bin Chenused to optimize the sensitivity of the method. A mouse IgG isotype control (ThermoFisher, catalog 31903) was used to confirm specificity of the method. 50,000 cells from each cell line were plated onto a

(12) Restoring the tumour mechanophenotype of vocal fold cancer reverts its malignant propertiesNature MaterialsFebruary 20, 2026Jasmin Kaivola, Karolina Punovuori, Megan R. Chastney, Hind Abdo, Gautier Follain, Mathilde Mathieu, Omkar Joshi, Yekaterina A. Miroshnikova, Fabian Krautgasser, Jasmin Di Franco, et al.culture medium supplemented with 10 µg ml−1 function blocking antibodies or IgG control; mouse anti-IgG (31903, Invitrogen), mouse anti-human α3 integrin (P1B5, in-house hybridoma), mouse anti-human α6 integrin

(13) R-loop editing by DNA cytosine deaminase APOBEC3B modulates the activity of oestrogen receptor enhancersNature CommunicationsFebruary 18, 2026Chi Zhang, Yu-jing Lu, Bingjie Chen, Zhiyan Bai, Qiaoxi Zeng, Alexia Hervieu, Marco P. Licciardello, Konstantinos Mitsopoulos, Bissan Al-Lazikani, Marcello Tortorici, et al.temperature 4 °C. S9.6 antibody was purchased from Kerafast (ENH002), and mouse IgG (Thermo Fisher 31903) was used as a control (if required). ssDRIP-seq was performed using immunoprecipitated DNA:RNA hybrids.

(14) Cyclic di-GMP suppresses cancer metastasis by targeting proteasome 26S subunit non-ATPase 3 independently of STINGSignal Transduct Target TherFebruary 4, 2026Jieqiong Wang, Alexander Mrozek, Kewen Hu, Hanyu You, Sarah E. Traverse, Hyemin Lee, Shelya X. Zeng, Xiufeng Pang, Heewon Park, Hua Lutotal protein was incubated with a specific antibody or control IgG (Thermo Fisher Scientific Cat# 31903, RRID:AB_10959891) overnight at 4 °C. The immune complexes were then incubated with protein G PLUS-Agarose

(15) ERα activates NAMPT/IL-33 signaling to enhance beige thermogenesis and metabolic fitnessScience AdvancesJanuary 9, 2026Ruoci Hu, Jooman Park, Yanyu Qian, Shaolei Xiong, Ahmad Hamza Elsabbagh, Aditya Chhikara, Huailing Fan, Zuoxiao Shi, Lifeng Liu, Yanhui Li, et al.TAM-induced 2-month-old mice were administered one dose of mouse IgG control (1 μg/mouse; Invitrogen 31903) or anti-IL-33 antibody (1 μg/mouse; R&D Systems AF3626) by intraperitoneal injection for five consecutive

(16) Targeting ubiquitin-specific protease 14 reduces metastatic potential and metabolic activity in cervical cancer via direct modulation of monocarboxylate transporter-4Journal of Translational MedicineDecember 2, 2025Ravi Chauhan, Gunjan Dagar, Lakshay Malhotra, Ashna Gupta, Anju Surendranath, Harshul Kapoor, Hemant Yadav, Rajat Ujjainiya, Suraja Kumar Das, Sameer Mirza, et al.mouse IgG isotype control (2 µg per 1000 µg protein) (Thermo Fisher Scientific, Mouse IgG Isotype Control, 31903). The next day, 20 µL of fresh Protein A/G beads were added and rotated for 2–3 h at 4 °C to capture

(17) Bisecting GlcNAc expression by bone marrow stromal cells modulates TGF-β1-driven macrophage polarization in myeloid leukemiasHaematologicaDecember 1, 2025Jingjing Feng, Junjie Gou, Yi Wang, Wei Wei, Yihan Ma, Xueting Ren, Chongfu Zhao, Xiaoliang Cheng, Lei Lei, Zengqi Tan, et al.(cat. #MHCD45054), CD3-PE (cat. #12003842), CD8-FITC (cat. #12003842), and isotype control antibodies (#31903/#31235, eBiosciences, San Diego, CA, USA). The stained cells were analyzed by flow cytometry (FACS;

(18) The late chromatoid body component TSSK2 is involved in translational regulation in elongating spermatids in miceReproduction (Cambridge, England)December 1, 2025Lehti MS, Ma L, Kärnä S, Laasanen S, Ahmedani A, Olotu O, Bourgery M, Tran P, Sironen A, Kotaja N.DDX4 (Abcam, UK; cat code ab13840), and negative control antibody (mouse IgG: Invitrogen, cat code 31903; rabbit IgG: Invitrogen, cat code 10500C) were added to the Dynabeads and incubated at RT for 2 h. Dynabeads-antibody

(19) Porphyromonas gingivalis Type 9 Secretion System Promotes Dysregulation of Vascular Smooth Muscle Cell Plasticity With Perturbed TGF-β/Smad SignalingMicrobiologyopenOctober 22, 2025Priscilla L. Phillips, Leticia Reyes(Catalog # 31883, Thermo Fisher Scientific) and mouse Immunoglobulin G (IgG) isotype control (Catalog # 31903, Thermo Fisher Scientific). All antibodies were diluted 1:200 in blocking buffer. Goat antirabbit ALEXA

(20) Integrated metabolomic and transcriptomic analysis identifies adipogenic differentiation of mesenchymal stem cells as a driver of chemoresistance in acute myeloid leukemiaJ Exp Clin Cancer ResOctober 17, 2025Zhipeng Pan, Rong Hu, Dandan Li, Siwen Deng, Haishan Yi, Zhengwei Duan, Lixia Kang, Ling Chen, Mengyao Wang, Yue Duan, et al.17-0739-42, 14-0909-82, 17-1057-42, eBiosciences, USA). PE-conjugated mouse IgG isotype control antibodies (31903, eBioscience, USA) were used in parallel to assess non-specific binding. Each marker was analyzed in

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Mouse IgG Isotype Control from Thermo Fisher Scientific

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Mouse IgG Isotype Control from Thermo Fisher Scientific

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