Western blot analysis of lysate from HT1080 cell line, using IFITM3 Antibody (N-term) (Cat. #TA324408).TA324408 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.

IF image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.