TPH2 Antibody PA1-778 from Thermo Fisher Scientific

Product Specs
ItemTPH2 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-778
NCBI Gene AliasesNtph, ADHD7, NTPH
HostRabbit
Quantity100 µL
ClonalityPolyclonal
ReactivityHuman, Mouse, Non-human primate, Rabbit, Rat
FormatPBS with 1mg/mL BSA and 0.1% sodium azide
ApplicationsImmunocytochemistry, Immunoprecipitation, Western Blot
TargetTPH2
Conjugate/TagUnconjugated
NCBI Full Gene Nametryptophan hydroxylase 2
ImmunogenSynthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)
IsotypeIgG
Storage Temperature4° C
PurityAffinity chromatography
Antibody TypePrimary
Concentration0.5 mg/mL
Storage4°C

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Involvement of the hypothalamus-raphe magnus-spinal defecation center axis in stress-induced defecation in ratsCommun BiolApril 1, 2026Natsufu Yuki, Tomoya Sawamura, Ayuna Mori, Hiroshi Yamaguchi, Yuuki Horii, Takahiko Shiina, Yasutake Shimizufollows: goat anti-GFP (1:200; ab6673, Abcam), rabbit anti-tryptophan hydroxylase 2 (TPH2) (1:1,000; PA1-778, Thermo Fisher), mouse anti-c-Fos (1:1000; ab208942), and rabbit anti-oxytocin (1:1000; 20068, ImmunoStar).

(2) Anatomical and Neuronal Distribution of the G‐Coupled Protein Receptor 75 mRNA in the Mouse Central Nervous SystemEuropean Journal of NeuroscienceFebruary 19, 2026Melanie K. Becher, Tessa Knox, Kaela Wilson, Lawrence F. Kromer, Italo Mocchetti(ThermoFisher Scientific, Cat. No. PA5‐85669); Tryptophan Hydroxylase 2, 1:250 (ThermoFisher Scientific, Cat. No. PA1‐778); vesicular Glutamate Transporter 1; and 1:100 (ThermoFisher Scientific, Cat. No. 48‐2400). Secondary

(3) Molecularly defined cellular atlas of the entire mouse brain with isotropic single-cell resolutionNature CommunicationsNovember 19, 2025Mengting Zhao, Jiandong Zhou, Tao Jiang, Miao Ren, Chuhao Dou, Lingyi Cai, Shengda Bao, Xueyan Jia, Zhaoyang Meng, Feifang Tang, et al.dilution; anti:TH, rabbit, Sigma-Aldrich, T8700, 1:1000 dilution; anti:TPH2, rabbit, Thermo Fisher, PA1-778, 1:400 dilution) for 12 h at 4 °C, followed by five washes in PBS at room temperature. Next, the sections

(4) Multi‐Region Brain Organoids Integrating Cerebral, Mid‐Hindbrain, and Endothelial SystemsAdvanced ScienceSeptember 1, 2025Anannya Kshirsagar, Hayk Mnatsakanyan, Sai Kulkarni, John Guo, Kai Cheng, Luke Daniel Ofria, Oce Bohra, Ram Sagar, Vasiliki Mahairaki, Christian E Badr, et al.paraformaldehyde (PFA) and subsequently immunostained for Phox2b (Rabbit anti‐Phox2b, ThermoFisher Scientific #PA1‐778), Beta‐Tubulin (Mouse anti‐Beta‐Tubulin, Sigma‐Aldrich #T8328), and CD31 (Rat anti‐CD31, BD Biosciences

(5) Distinct subnetworks of the mouse anterior thalamic nucleiNat CommunJuly 1, 2025Houri Hintiryan, Mitchell Rudd, Sumit Nanda, Adriana E. Gutierrez, Darrick Lo, Tyler Boesen, Luis Garcia, Jiandong Sun, Christian Estrada, Hyun-Seung Mun, et al.anti-tryptophan hydroxylase (TPH2) to identify serotonin positive neurons (ThermoFisher Scientific, #PA1-778] for 48–72 h at 4 °C. Following three KPBS rinses, sections were soaked for 3 h in the secondary antibody

(6) Hyperphenylalaninemia and serotonin deficiency in Dnajc12-deficient miceCommunications biologyDecember 18, 2024Cao Y, Popp O, Milani N, Qadri F, Kühn R, Mertins P, Bader M, Alenina N.Hydroxylase,1:1000,Mouse,Sigma,T0678-WH3 TPH1 (D10C10),1:1000,Rabbit,ThermoFisher,mAb#12339 TPH2,1:1000,Rabbit,ThermoFisher,PA1-778 GAPDH (14C10),1:1000,Rabbit,Cell Signaling,2118 PAH,1:1000,Goat,Santa Cruz,SC15112 Secondary antibody,Secondary

(7) Sex-specific modulation of the medial prefrontal cortex by glutamatergic median raphe neuronsScience advancesNovember 10, 2023Collins SA, Stinson HE, Himes A, Nestor-Kalinoski A, Ninan I.(Rockland Immunochemicals, catalog no. 600401P16) or Tph2 polyclonal antibody (Invitrogen, catalog no. PA1-778, RRID: AB_2207687) at a dilution of 1:1000 in 0.3% Triton X-100/TBS for 24 hours at 4°C (70, 71). Slices

(8) Elevated levels of serotonin 5-HT(2A) receptors in the orbitofrontal cortex of antisocial individualsEuropean archives of psychiatry and clinical neuroscienceMarch 1, 2023Braccagni G, Scheggi S, Bortolato M.1:750); anti-5-HT4 (rabbit polyclonal #ab60359 Abcam; dilution 1:1000); anti-TPH2 (rabbit polyclonal #PA1–778 Thermo Fisher Scientific, Waltham, MA; dilution 1:750) were incubated in TBS-T containing 3% (w/v) BSA

(9) Slitrk2 deficiency causes hyperactivity with altered vestibular function and serotonergic dysregulationiScienceJuly 15, 2022Katayama KI, Morimura N, Kobayashi K, Corbett D, Okamoto T, Ornthanalai VG, Matsunaga H, Fujita W, Matsumoto Y, Akagi T, et al.1:1000), anti-SERT (Slc6A4) antibody (HTT-Rb-Af560, Frontier Institute, 1:300), and anti-Tph2 antibody (PA1-778, ThermoFisher, 1:500). The goat anti-SLITRK2 antibody shows approximately 10% cross-reactivity with

(10) The Mesoscopic Connectome of the Cholinergic Pontomesencephalic TegmentumFrontiers in neuroanatomyJanuary 1, 2022Zhao P, Wang H, Li A, Sun Q, Jiang T, Li X, Gong H.T8700), and anti-Tryptophan hydroxylase 2 (1:1,000, rabbit, Invitrogen, Carlsbad, CA, United States, PA1-778). Then, the sections were washed in PBS 5 times (5 min each) at room temperature. Next, these sections

(11) Sex and brain region-specific regulation of serotonin transporter activity in synaptosomes in guanine nucleotide-binding protein G(q) alpha knockout miceJournal of neurochemistryOctober 1, 2021Haase J, Jones AKC, Mc Veigh CJ, Brown E, Clarke G, Ahnert-Hilger G.

(12) Sexually dimorphic response of colorectal motility to noxious stimuli in the colorectum in ratsThe Journal of physiologyMarch 1, 2021Horii K, Ehara Y, Shiina T, Naitou K, Nakamori H, Horii Y, Shimaoka H, Saito S, Shimizu Y.

(13) Depletion of TrkB Receptors From Adult Serotonergic Neurons Increases Brain Serotonin Levels, Enhances Energy Metabolism and Impairs Learning and MemoryFrontiers in molecular neuroscienceJanuary 1, 2021Sahu MP, Pazos-Boubeta Y, Steinzeig A, Kaurinkoski K, Palmisano M, Borowecki O, Piepponen TP, Castrén E.MAB377X, Millipore), GFAP (Catalog number 12389, Cell Signaling Technology), and Tph2 (Catalog number PA1-778, Thermo Fisher Scientific). The respective secondary antibodies were Alexa conjugated antibodies (Invitrogen).

(14) Combined exposure to dinotefuran and chronic mild stress counteracts the change of the emotional and monoaminergic neuronal activity induced by either exposure singly despite corticosterone elevation in miceThe Journal of veterinary medical scienceMarch 24, 2020Takada T, Yoneda N, Hirano T, Onaru K, Mantani Y, Yokoyama T, Kitagawa H, Tabuchi Y, Nimako C, Ishizuka M, et al.polyclonal antibody against TPH2,EnVision+ System- HRP Labeled Polymer ,"(Nacalai Tesque, Kyoto, Japan)","(PA1-778, 1:4,000)",Anti-Rabbit ,,"(Thermo Fisher, Waltham, MA, USA)","(Dako, Glostrup, Denmark)" ,,, TH,Blocking reagent

(15) Serotonergic Plasticity in the Dorsal Raphe Nucleus Characterizes Susceptibility and Resilience to AnhedoniaThe Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 15, 2020Prakash N, Stark CJ, Keisler MN, Luo L, Der-Avakian A, Dulcis D.(secondary) 1:1000 1:2000 1:1000 1:500 1:100 1:500 1:500 1:500 1:500 1:500 1:300 1:500 sc-166940 (Santacruz Biotech) ABN90 (Millipore Sigma) AB1542 (Millipore Sigma) PA1–778 (ThermoFisher Scientific) AB5421-I (Millipore Sigma) AB1529 (Millipore Sigma) A21448 (Invitrogen) A11015

(16) Differential role of dose and environment in initiating and intensifying neurotoxicity caused by MDMA in ratsBMC pharmacology & toxicologyAugust 5, 2019Shokry IM, Shields CJ, Callanan JJ, Ma Z, Tao R.Polyclonal rabbit anti-TpH2 antibody obtained from Thermo Scientific (Rockford, IL, USA; product No. PA1–778) was used at a dilution of 1: 1,000, and Alexa Fluor 594-conjugated goat anti-rabbit IgG from Invitrogen

(17) Protection of tissue physicochemical properties using polyfunctional crosslinkersNature biotechnologyDecember 17, 2018Park YG, Sohn CH, Chen R, McCue M, Yun DH, Drummond GT, Ku T, Evans NB, Oak HC, Trieu W, et al.Abcam, MA), rabbit anti-calbindin (ab11426, Abcam, MA), chicken anti-TH (TYH, Aves), or mouse anti-TPH2 (PA1–778, Thermo), in 1X PBST overnight at RT using 1:300 dilution factors for all but NFL (1:150), washed in

(18) Motherhood and infant contact regulate neuroplasticity in the serotonergic midbrain dorsal raphePsychoneuroendocrinologyFebruary 1, 2017Holschbach MA, Lonstein JS.solutions: 1) primary antiserum diluted 1:500 in iBind solution (rabbit anti-TPH2, Thermo Scientific PA1-778, Rockford, IL), 2) iBind solution, and 3) secondary antiserum diluted 1:1000 in iBind Solution (goat

(19) Associations among within-litter differences in early mothering received and later emotional behaviors, mothering, and cortical tryptophan hydroxylase-2 expression in female laboratory ratsHormones and behaviorJanuary 1, 2016Ragan CM, Harding KM, Lonstein JS.conducted as previously described (Ragan and Lonstein, 2014) using a TPH2 primary antiserum (1:1000, #PA1-778, Lot: PB195015, Thermo Fisher Scientific, Rockford, IL) with a Super Signal Western Blot Enhancer kit

(20) Sympathetic regulation of vascular tone via noradrenaline and serotonin in the rat carotid body as revealed by intracellular calcium imagingBrain researchJanuary 30, 2015Yokoyama T, Nakamuta N, Kusakabe T, Yamamoto Y.

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TPH2 Antibody from Thermo Fisher Scientific

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TPH2 Antibody from Thermo Fisher Scientific

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