CD45 Antibody (30-F11) - BSA Free NB100-77417 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemCD45 Antibody (30-F11) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberNB100-77417
Concentration1.0 mg/ml
ImmunogenThis CD45 Antibody (30-F11) was prepared from Mouse thymus or spleen
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Functional,In vitro assay,In vivo assay,CyTOF-ready,Functional Assay,Immunocytochemistry/ Immunofluorescence
ReactivityHuman,Mouse,Rat
HostRat
Quantity0.5 mg (also 0.1 mg)
TargetCD45
ClonalityMonoclonal
IsotypeIgG2b Kappa
Conjugate/TagUnconjugated
Clone30-F11
FormatPBS
PurityProtein G purified
Research AreaAdaptive Immunity, Autoimmune Diseases, Cell Biology, Cellular Markers, Cellular Signaling, Cytokine Research, Glia Markers, Growth and Development, Hematopoietic Stem Cell Markers, Immunology, Inflammation, Innate Immunity, MAP Kinase Signaling, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Modulation of DNA Pools, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
NCBI Full Gene Nameprotein tyrosine phosphatase receptor type C
NCBI Gene AliasesLY5, GP180, loc, CD45, L-CA, Cd45, CD45R, LCA, IMD105, T200, Lyt-4, B220, Ly-5
Antibody TypePrimary Antibodies
Molecular Weight145 kDa
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b)

Sizes Available: 0.5 mg (also 0.1 mg)

Add to Compare List

Questions and Answers

What applications has the CD45 antibody been used for in recent research?

The CD45 antibody (30-F11) has been utilized in various applications including immunohistochemistry, immunofluorescence, and flow cytometry. For instance, it was employed in a study to analyze leukocyte infiltration in tissues during enterovirus infection in a mouse model, highlighting its role in identifying immune cell populations (). Additionally, it has been used in studies investigating intimal hyperplasia and re-endothelialization in saphenous vein grafts, demonstrating its utility in vascular biology ().

Does this antibody recognize all isoforms of CD45, and how has this been demonstrated?

Yes, the CD45 antibody recognizes all CD45 isoforms as well as known mouse CD45 alloantigens. This specificity has been confirmed in multiple studies, including its application in immunohistochemistry to identify CD45-positive cells in various tissue contexts, such as in the lungs during injury and repair processes (, ).

Can this antibody be used in studies involving mouse models, and if so, how?

The CD45 antibody has been effectively used in mouse models to study immune responses and tissue regeneration. For example, it was used to assess immune cell infiltration in a model of lung injury, where it helped characterize the immune landscape during the repair process (). Furthermore, it has been cited in research focusing on pancreatic islet regeneration in diabetic mouse models, indicating its relevance in metabolic studies ().

What species does the CD45 antibody react with, and how has this been validated in research?

The CD45 antibody is reactive with mouse, human, and rat species. Its cross-reactivity has been validated in various studies, including those examining immune cell dynamics in both murine and human tissues, thereby supporting its use in comparative immunological research (, ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Effectiveness of a heparin-like glycosaminoglycan from snail mucus in a murine model of dry eyeJ Transl MedJuly 21, 2026Shangkun Ou, Lingli Zhang, Lisha Lin, Liying Zhang, Hao Jiang, Qiurong Long, Meng Zhang, Xueer Zheng, Xiaoyu Tian, Yiming Wu, et al.(24789-1-AP), K14 (82824-1-AP) and PAX6 (12323-1-AP) antibodies were from Proteintech (China), CD45 (NB100-77417) antibody was from Novus (USA), and F4/80 (HA721520) and GAPDH (ET1601-4) antibodies were from Huabio

(2) Diet-dependent modulation of energy balance by CB1 signaling in peripheral sensory neuronsiScienceAugust 15, 2025Linden B, Herz H, Jarrah M, Tasabehji D, Saleh S, Fraer A, Clark P, Ye Y, Chu Y, Al-Khalil Z, et al.anti-CB1 1:250,Abcam,ab23703 Rat anti-mouse CD68 1:50,Bio-Rad,MCA1957GA Rat anti-CD45 1:50,Novus,NB100-77417 Rabbit anti-TH 1:200,Phosphosolutions,#2025-THRAB Rabbit anti-CGRP 1:1000,Sigma,C8198 Rabbit anti-chromogranin

(3) Lymphatic Activation of ACKR3 Signaling Regulates Lymphatic Response After Ischemic Heart InjuryArteriosclerosis, Thrombosis, and Vascular BiologyMay 1, 2025Laszlo Balint, Shubhangi Patel, Donald Stephen Serafin, Hua Zhang, Kelsey E. Quinn, Amir Aghajanian, Bryan M. Kistner, Kathleen M. Caronantibodies: rabbit anti-LYVE1 (1:200, Fitzgerald, 70R-LR005), goat anti-CD45 (1:50, Novus Biologicals, NB100-77417), goat anti-F4/80 (1:50, BD Pharminogen, 565409), donkey anti-rabbit Cy3 (1:200, Jackson ImmunoResearch,

(4) Lymphatic activation of ACKR3 signaling regulates lymphatic response after ischemic heart injuryArterioscler Thromb Vasc BiolMarch 27, 2025Laszlo Balint, Shubhangi Patel, D. Stephen Serafin, Hua Zhang, Kelsey E. Quinn, Amir Aghajanian, Bryan M. Kistner, Kathleen M. Caronantibodies: rabbit anti-LYVE1 (1:200, Fitzgerald, 70R-LR005), goat anti-CD45 (1:50, Novus Biologicals, NB100-77417), goat anti-F4/80 (1:50, BD Pharminogen, 565409), donkey anti-rabbit Cy3 (1:200, Jackson ImmunoResearch,

(5) An injury-induced mesenchymal-epithelial cell niche coordinates regenerative responses in the lungScienceDecember 13, 2024Dakota L. Jones, Michael P. Morley, Xinyuan Li, Yun Ying, Gan Zhao, Sarah E. Schaefer, Luis R. Rodriguez, Fabian L. Cardenas-Diaz, Shanru Li, Su Zhou, et al.Santa Cruz, cat# sc-515825), Jag2 (rabbit, LSBio, cat# LS‑C100395), CD45 (rat, Novus Biologicals, cat# NB100–77417), Pdgfra (goat, R&D, cat# AF1062), Pdgfrb (goat, R&D, cat # AF1062), and Erg (mouse, Abcam, cat# ab214341). For

(6) Exploring the Potential of Saphenous Vein Grafts Ex Vivo: A Model for Intimal Hyperplasia and Re-EndothelializationJournal of Clinical MedicineAugust 14, 2024Nur A’tiqah Haron, Mohamad Fikeri Ishak, Muhammad Dain Yazid, Ubashini Vijakumaran, Roszita Ibrahim, Raja Zahratul Azma Raja Sabudin, Hafiza Alauddin, Nur Ayub Md Ali, Hairulfaizi Haron, Muhammad Ishamuddin Ismail, et al.Centennial, CO, USA), CD133-1:250, (NB120-16518, Novus Biologicals, Centennial, CO, USA), and CD45-1:100 (NB100-77417, Novus Biologicals, Centennial, CO, USA). Secondary antibodies include goat anti-mouse 488-1:100 (ab150077,

(7) An injury-induced tissue niche shaped by mesenchymal plasticity coordinates the regenerative and disease response in the lungbioRxivMarch 5, 2024Dakota L. Jones, Michael P. Morley, Xinyuan Li, Yun Ying, Fabian L. Cardenas-Diaz, Shanru Li, Su Zhou, Sarah E. Schaefer, Ullas V. Chembazhi, Ana Nottingham, et al.Cell Signaling, cat# 4147), Jag2 (rabbit, LSBio, cat# LS-C100395), CD45 (rat, Novus Biologicals, cat# NB100-77417). For RNAscope, lung tissue was prepared, fixed, embedded in paraffin, and sectioned as described above.

(8) Murine liver repair via transient activation of regenerative pathways in hepatocytes using lipid nanoparticle-complexed nucleoside-modified mRNANature communicationsJanuary 1, 0001"Rizvi F, Everton E, Smith AR, Liu H, Osota E, Beattie M, Tam Y, Pardi N, Weissman D, Gouon-Evans V."

(9) Overexpression of HOX11 leads to the immortalization of embryonic precursors with both primitive and definitive hematopoietic potentialBloodJanuary 1, 0001"Keller G, Wall C, Fong AZ, Hawley TS, Hawley RG."

(10) Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised micePLoS pathogensJanuary 1, 0001"Simanjuntak Y, Ko HY, Lee YL, Yu GY, Lin YL."

(11) Chronic liver inflammation and hepatocellular carcinogenesis are independent of S100A9International journal of cancerJanuary 1, 0001"De Ponti A, Wiechert L, Stojanovic A, Longerich T, Marhenke S, Hogg N, Vogel A, Cerwenka A, Schirmacher P, Hess J, et al."

(12) Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease"Journal of immunology (Baltimore, Md. : 1950)"January 1, 0001"Seaman WE, Wofsy D, Greenspan JS, Ledbetter JA."

(13) Bioprinted 3D Bionic Scaffolds with Pancreatic Islets as a New Therapy for Type 1 Diabetes-Analysis of the Results of Preclinical Studies on a Mouse ModelJournal of functional biomaterialsJanuary 1, 0001"Klak M, Wszoła M, Berman A, Filip A, Kosowska A, Olkowska-Truchanowicz J, Rachalewski M, Tymicki G, Bryniarski T, Kołodziejska M, et al."sc-514592; Santa Cruz Biotechnology, Dallas, TX, USA), CD45 (Novus Biologicals, Centennial, CO, USA, NB100-77417, 1:500), or MOMA (Novus Biologicals, Centennial, CO, USA; NB100-64946, 1:500) overnight at 4 °C. The

(14) Transient yet Robust Expression of Proteins in the Mouse Liver via Intravenous Injection of Lipid Nanoparticle-encapsulated Nucleoside-modified mRNABio-protocolJanuary 1, 0001"Everton E, Rizvi F, Smith AR, Beattie M, Tam Y, Pardi N, Weissman D, Gouon-Evans V."

(15) BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy EfficacyCell reportsJanuary 1, 0001"Samanta D, Huang TY, Shah R, Yang Y, Pan F, Semenza GL."

(16) An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapiesBiomaterialsJanuary 1, 0001"Wang J, Kural MH, Wu J, Leiby KL, Mishra V, Lysyy T, Li G, Luo J, Greaney A, Tellides G, et al."

(17) Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cellsCancer researchJanuary 1, 0001"Hanaoka K, Fujita N, Lee SH, Seimiya H, Naito M, Tsuruo T."

(18) Bone Marrow-Derived Cell Recruitment to the Neurosensory Retina and Retinal Pigment Epithelial Cell Layer Following Subthreshold Retinal PhototherapyInvestigative ophthalmology & visual scienceJanuary 1, 0001"Caballero S, Kent DL, Sengupta N, Li Calzi S, Shaw L, Beli E, Moldovan L, Dominguez JM 2nd, Moorthy RS, Grant MB."

(19) Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasionCellJanuary 1, 0001"Gangoso E, Southgate B, Bradley L, Rus S, Galvez-Cancino F, McGivern N, Güç E, Kapourani CA, Byron A, Ferguson KM, et al."

(20) Selective radiation of hematolymphoid tissue delivered by anti-CD45 antibodyCancer researchJanuary 1, 0001"Matthews DC, Badger CC, Fisher DR, Hui TE, Nourigat C, Appelbaum FR, Martin PJ, Bernstein ID."

Be the First to Write a Review!