Immunofluorescent analysis of NF-H in a mouse hippocampus section. The mouse brain section was obtained following transcardial perfusion of the mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained using an NF-H polyclonal antibody (Product # PA1-10016) at a dilution of 1:2,000 as seen in red, costained using an MBP monoclonal antibody at a dilution of 1:5,000 as seen in green, and with DAPI staining the nuclear DNA in blue. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.