Fig 2: AVP and its receptors in the medial cerebellar nucleus (Med). The large neurons of Med showed AVP (a), V1aR (b) and V1bR (c) immunoreactivity in the neuronal cytoplasm

Fig 3: Co-localization of vasopressin and its receptors with RAMP1 in TG. a Double staining of vasopressin and RAMP1, b double staining of V1aR and RAMP1, c double staining of V1bR and RAMP1. Vasopressin, V1aR and V1bR co-localized with RAMP1 in the neuronal cytoplasm (arrows)

Fig 4: Immunohistochemistry of AVP, V1aR, V1bR and V2R in TG from male and female rat. AVP immunoreactivity was located in neuronal cytoplasm (arrow) and in SGCs (arrowhead) and of all neurons, 36% were positive for AVP in males and 37% in females. In males, 76% of all neurons were V1aR immunoreactive and 75% V1bR immunoreactive. In females, 86% of all neurons were positive for V1aR and 84% of all neurons were positive for V1bR. V1aR and V1bR were located in neuronal cytoplasm (arrow) and in fibers (arrowheads). V2R immunoreactivity was expressed mostly in the SGCs (arrowhead) and a fewer in the neuronal cytoplasm (arrow)

Fig 5: Expression of AVP, V1aR and V1bR mRNA in hypothalamus and trigeminal ganglion. a The mRNA levels of AVP, V1aR and V1bR in HT and, b in TG. Expression was normalized to housekeeping gene GAPDH. Data were obtained by real-time PCR and are expressed as mean ± s.e.m., n = 6, *P < 0.05, **P < 0.01 and ***P < 0.001