Immunoprecipitation: ILKAP/PP2C delta Antibody [NB100-74606] - ILKAP Antibody [NB100-74606] - Detection of Human ILKAP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ILKAP antibody NB100-74606 used for IP at 3 mcg/mg lysate. ILKAP was also immunoprecipitated by rabbit anti- ILKAP antibody NB100-74607, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.