Alpha B Crystallin Antibody

Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (SPC-126). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (SPC-126) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (SPC-126). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (SPC-126) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (SPC-126). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (SPC-126) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.