Anti-H+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody) AS07 260 from Agrisera

Product Specs
ItemAnti-H+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody)
CompanyAgrisera
Price EUR329.00Supplier Page View Company Product Page
Catalog NumberAS07 260
Quantity50 µg
ImmunogenKLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456, TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
ReactivityActinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata, Ricinus communis, Salicornia bigelovii, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Vicia faba, Zea mays
ClonalityPolyclonal
FormatLyophilized
HostRabbit
ApplicationsImmunofluorescence, Immunolocalization, Western blot
TargetAnti-H+ATPase
Research AreaPlant Biology
PurityImmunogen affinity purified serum in PBS pH 7.4
NCBI Gene AliasesPLASMA MEMBRANE PROTON ATPASE, OPEN STOMATA 2, AHA4, AHA6, AHA7, PMA2, AHA1, AHA2, AHA3, AHA8, AHA9, F23A5.1, F19F24_16, H(+)-ATPase 7, MJB24.16, PMA, ARABIDOPSIS THALIANA ARABIDOPSIS H(+)-ATPASE, OST2, H(+)-ATPase 11, AHA11, T8B10.1, ATAHA3, H(+)-ATPase 9, F9N11_40, H(+)-ATPase 2, H(+)-ATPase 3, H(+)-ATPase 1, H(+)-ATPase 6, F9N11.40, H(+)-ATPase 4, F9A16_7, MRG21.9, MJB24_16, H(+)-ATPase 8, F23A5_1, F9A16.7, P-TYPE H(+)-ATPASE ISOFORM 3, P-TYPE H(+)-ATPASE ISOFORM 2, MRG21_9, F19F24.16, PLASMA MEMBRANE PROTON ATPASE 2
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C
Molecular Weight90-95 kDa (Arabidopsis thaliana, depending upon an isoform)

Specifications/Features

1: 1000 - 1: 5000 with standard ECL OR 1: 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1: 600 - 1: 1000 (IF), 1: 100 (IL)

Add to Compare List

Questions and Answers

Can this H+ATPase antibody be used for immunoblotting in plant studies?

Yes, the H+ATPase antibody has been successfully utilized in immunoblotting applications, as demonstrated in various studies, including those involving Arabidopsis thaliana and Chlamydomonas reinhardtii, where it was used to detect plasma membrane H+-ATPase levels under different experimental conditions.

What types of organisms does this antibody react with?

The H+ATPase antibody shows reactivity with a range of organisms, including various plants such as Arabidopsis, maize, and tobacco, as well as algae and zebrafish, making it versatile for studies across different species in plant biology.

Is there any evidence of this antibody being used in research related to plant immunity?

Yes, the H+ATPase antibody has been referenced in studies focusing on plant immunity, such as in the research on the immune receptor FLAGELLIN SENSING2, where it was used to analyze the abundance of defense proteins at the plasma membrane, highlighting its role in immune signaling pathways.

How has this antibody been applied in studies involving carbon dioxide tolerance in microalgae?

The H+ATPase antibody has been employed in research examining the effects of high CO2 environments on microalgae, specifically in Chlamydomonas reinhardtii, where it was used to assess the expression levels of plasma membrane H+-ATPases, which are crucial for maintaining cellular pH and metabolic functions under stress conditions.

Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) Expanding the Analytical Toolbox for Extracellular Vesicle Biochemical Profiling: A Multiplatform Spectroscopic and Chromatographic StrategyAnalytical ChemistryMay 12, 2026Caterina Branca, Angela Paterna, Estella Rao, Samuele Raccosta, Mohamed Zekri, Sabrina Picciotto, Paola Gargano, Giorgia Adamo, Luana Pulvirenti, Laura Siracusa, et al.antibody anti H+-ATPase (diluted 1:1000 in blocking buffer, diluted 1:1 with TBS/Tween 0.2%, Agrisera, AS07260), antibody anti Alix (diluted 1:200 in blocking buffer, diluted 1:1 with TBS/Tween 0.2%, Santa Cruz Biotechnology Inc

(2) Cellular Responses of Maize Roots to Long-Term Cadmium Exposure: Adjustments of Class III Peroxidases, Plasma Membrane and Tonoplast Sub-ProteomesProteomesFebruary 25, 2026Sabine Lüthje, Ayse Gül Yilmaz, Kalaivani Ramanathan, Waldemar Gräfenstein, Jenny M. Tabbert, Stefanie Wienkoop, Katrin Heino, François Clement Perrineau, Sönke HarderAgrisera, Vännäs, Sweden) were used: after separation by 10% SDS-PAGE for plasma membrane H+-ATPase (AS07 260; 1:1000) and vacuolar H+-pyrophosphatase (V-PPase, AS12 1849; 1:2000); after separation on 14% SDS-PAGE

(3) Light-induced OsLIKE1 phosphorylation enhances rice resistance against blast diseaseNature CommunicationsDecember 23, 2025Jiangtao Liu, Lei Su, Wenya Wang, Jintao Wang, Weizhong Chen, Zhixiang Yang, Leiyun Yang, Gang Li, Xinyu Liu, Haifeng Zhang, et al.isolated membrane protein was used for western blot analysis with the H+ATPase antibody (Agrisera, AS07260). Nuclear and Cytoplasmic Protein Extraction: Rice protoplasts were resuspended in pre-cooled cell lysis

(4) Cleavage of Bcl-2-associated athanogene by metacaspase determines plant antiviral immunityNat CommunOctober 14, 2025Lin Liang, Yuhang Jiang, Pingzhi Zhao, Hongwei Wang, Xiaoyue Chen, Xiao Lin, Yanwei Sun, Wenqian Zhang, Rongxiang Fang, Jian Yeplasma membrane marker protein, H+-ATPase, was detected using an anti-H+-ATPase monoclonal antibody (Agrisera, AS07 260; 1:5000 dilution). The uncropped blots of immunoblotting images are provided in the Source Data files. Ion

(5) The ESCRT component FYVE4 modulates salt-stress response by strengthening the SOS1–SOS2 interaction in ArabidopsisPlant CommunicationsSeptember 8, 2025Chuanliang Liu, Xinyi Lin, Min Xu, Zhao Zheng, Zhenghao Wang, Xin Huang, Feihua Wu, Guoyong Liu, Weijie Liu, Changlian Peng, et al.antibody (cat. no. PHY3509A) was purchased from PhytoAB. Rabbit antibodies against H+-ATPase (cat. no. AS07 260) and FBPase (cat. no. AS04 043) were obtained from Agrisera. The GFP mouse antibody (cat. no. HT801-01)

(6) Subcellular targets and recognition mechanism of Ralstonia solanacearum effector RipE1iScienceMay 16, 2025Dimitra Tsakiri, Konstantinos Kotsaridis, Vassiliki A. Michalopoulou, Ning Zhang, Sotiris Marinos, Nikos Kountourakis, Michael Kokkinidis, Gregory B. Martin, Panagiotis F. Sarristransiently expressing proteins of interest (72hpi). H+-ATPase was detected with an anti-H+ -ATPase (AS07 260; Agrisera) antibody and the cytosolic fraction was verified by staining of the polyvinylidene difluoride

(7) Role of polar localization of the silicon transporter OsLsi1 in metalloid uptake by rice rootsPlant PhysiolMay 9, 2025Noriyuki Konishi, Namiki Mitani-Ueno, Jian Feng Maantibody (1:1,000 dilutions; Shao et al. 2021), and rabbit polyclonal H+-ATPase antibody as a control (AS07 260, 1/5,000, Agrisera), respectively. Anti-Rabbit IgG (H + L) HRP Conjugate (1:50,000; Promega, http://www.promega.com)

(8) Lack of AtMC1 catalytic activity triggers autoimmunity dependent on NLR stabilityEMBO ReportsMarch 20, 2025Jose Salguero-Linares, Laia Armengot, Joel Ayet, Nerea Ruiz-Solaní, Svenja C Saile, Marta Salas-Gómez, Esperanza Fernandez, Lode Denolf, Fernando Navarrete, Jenna Krumbach, et al.RRID:AB_10751750), α-cAPXa (dilution 1:5000, Agrisera Cat# AS23 4940), α-H+ATPase (dilution 1:5000 Agrisera Cat# AS07 260, RRID:AB_1031584), α-SOBIR1 (dilution 1:1000, Agrisera Cat# AS16 3204), α-BIK1 (1:3000 Agrisera Cat#

(9) The role of indole‐3‐acetic acid and characterization of PIN transporters in complex streptophyte alga Chara brauniiNew PhytologistMarch 6, 2025Katarina Kurtović, Stanislav Vosolsobě, Daniel Nedvěd, Karel Müller, Petre Ivanov Dobrev, Vojtěch Schmidt, Piotr Piszczek, Andre Kuhn, Adrijana Smoljan, Tom J. Fisher, et al.into acidic and alkaline segments; overnight incubated at 4°C with rabbit anti‐H+‐ATPase (1 : 1000, AS07260; Agrisera, Vännäs, Sweden), polyclonal rat anti‐CbPINa or anti‐CbPINc (1 : 500; Moravian Biotechnology,

(10) The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum-Plasma Membrane Contact Sites and Interacts With AVRPM3(b2/c2) Through Its LRR DomainMolecular plant pathologyFebruary 1, 2025Isaksson J, Kunz L, Flückiger S, Widrig V, Keller B.Membranes were probed with HRP‐conjugated primary anti‐HA (clone 3F10, 12,013,819,001, Roche), anti‐ATPase (AS07 260, Agrisera), followed by HRP‐conjugated anti‐rabbit (sc‐2357, LabForce) or anti‐HSP70 (1D9, Enzo), followed

(11) ATG8 delipidation is not universally critical for autophagy in plantsNat CommunJanuary 5, 2025Yong Zou, Jonas A. Ohlsson, Sanjana Holla, Igor Sabljić, Jia Xuan Leong, Florentine Ballhaus, Melanie Krebs, Karin Schumacher, Panagiotis N. Moschou, Simon Stael, et al.(Agrisera, AS14 2769) were used at a dilution of 1:5000, while anti-rabbit HRP-conjugate (Agrisera, AS07 260) at a dilution of 1:10,000. Densitometric analysis Densitometric analysis of the western blots was

(12) GhCASPL1 regulates secondary cell wall thickening in cotton fibers by stabilizing the cellulose synthase complex on the plasma membraneJournal of Integrative Plant BiologyDecember 1, 2024Li Zhang, Xingpeng Wen, Xin Chen, Yifan Zhou, Kun Wang, Yuxian ZhuBIOTECH, Suzhou, China, Cat. No. YKZP902, 1:10,000 dilution), anti‐H+‐ATPase (Agrisera, Sweden, Cat. No. AS07260, 1:3,000 dilution), and anti‐GhCesA8 (our laboratory, 1:10,000 dilution). Peroxidase‐conjugated goat

(13) A land plant‐specific VPS13 mediates polarized vesicle trafficking in germinating pollenNew PhytologistDecember 1, 2024Surachat Tangpranomkorn, Yuka Kimura, Motoko Igarashi, Fumiko Ishizuna, Yoshinobu Kato, Takamasa Suzuki, Takuya Nagae, Sota Fujii, Seiji Takayama(AS04 053A; Agrisera), anti‐BiP (AS09 481; Agrisera), anti‐Sec21p (AS08 327; Agrisera), anti‐H+‐ATPase (AS07 260; Agrisera), anti‐SEC15A (PHY0818A; PhytoAB, San Jose, CA, USA), anti‐GFP (no.: 598; MBL, Tokyo, Japan),

(14) Dual function of overexpressing plasma membrane H + -ATPase in balancing carbon-water useScience AdvancesNovember 8, 2024Hangjin Jiang, Jinghan Su, Zirong Ren, Dexian Wang, Adrian Hills, Toshinori Kinoshita, Michael R. Blatt, Yin Wang, Yizhou Wangcentrifuged (14,000 rpm) for 10 min. Antibodies were obtained from Agrisera (plasma membrane H+-ATPase, AS07260, 1:5000 dilution) and Abcam (immunoglobulin G–horseradish peroxidase ab6721, 1:5000 dilution). Blots

(15) E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditionsPlant PhysiologyMay 31, 2024Jun Soo Kwak, Jong Tae Song, Hak Soo Seoanti-histone 3 (ab1791, Abcam), anti-tubulin (sc-33999, Santa Cruz Biotechnology), and anti-H+-ATPase (AS07260, Agrisera) antibodies, respectively. Effects of SSV2 overexpression on CNGC6 concentration in vivo Fourteen-day-old

(16) An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and functionNature CommunicationsMay 30, 2024Yuying Li, Qiong Wang, Huimin Jia, Kazuya Ishikawa, Ken-ichi Kosami, Takahiro Ueba, Atsumi Tsujimoto, Miki Yamanaka, Yasuyuki Yabumoto, Daisuke Miki, et al.immunoblotting with anti-c-Myc (9B11; Cell Signaling Technology), anti-cAPX (AS06 180; Agrisera), anti-H+ATPase (AS07 260; Agrisera) and anti-FLAG (F3165; Sigma) antibodies. Cell fractionation Microsomal fractionation from

(17) SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigeloviiNature CommunicationsMay 20, 2024Octavio R. Salazar, Ke Chen, Vanessa J. Melino, Muppala P. Reddy, Eva Hřibová, Jana Čížková, Denisa Beránková, Juan Pablo Arciniegas Vega, Lina María Cáceres Leal, Manuel Aranda, et al.PsbA (catalog number AS05 084) 1:10000 working dilution; Plasma membrane H+ ATPase (catalog number AS07 260) 1:1000 working dilution; and Tonoplast V-ATPase (catalog number AS07 213) 1:2000 working dilution.

(18) Thermoprotection by a cell membrane–localized metacaspase in a green algaThe Plant CellFebruary 26, 2024Yong Zou, Igor Sabljić, Natalia Horbach, Adrian N Dauphinee, Anna Åsman, Lucia Sancho Temino, Elena A Minina, Marcin Drag, Simon Stael, Marcin Poreba, et al.antibody was from Santa Cruz Biotechnology (sc-805). The anti-H+ ATPase antibody was from Agrisera (AS07 260). The membranes were blocked in 5% (w/v) nonfat-dried milk dissolved in Tris-buffered saline with 0.1%

(19) Arabidopsis COP1 guides stomatal response in guard cells through pH regulationCommunications BiologyFebruary 5, 2024Seoyeon Cha, Wang Ki Min, Hak Soo SeoSDS-PAGE, PM H+-ATPase protein levels were examined by western blotting with anti-PM H+-ATPase antibody (AS07-260; Agrisera). The same PVDF membrane used in the western blotting was stained with Coomassie Brilliant

(20) Spatiotemporal deposition of cell wall polysaccharides in oat endosperm during grain developmentPlant PhysiologyOctober 20, 2023Pavani P Nadiminti, Sarah M Wilson, Allison van de Meene, Alfie Hao, John Humphries, Julian Ratcliffe, Changyu Yi, Marta Peirats-Llobet, Mathew G Lewsey, James Whelan, et al.incubated in an appropriate antimouse secondary antibody. A rabbit polyclonal H+-ATPase (Agrisera, AS07260) was used at a 1:100 dilution in combination with an Alex Fluor 568 antirabbit IgG (H+L) secondary antibody

Be the First to Write a Review!

Anti-H+ATPase : Plasma membrane H+ATPase (rabbit antibody)

Anti-H+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody) from Agrisera

Description

Specifications/Features

Questions and Answers

Agrisera

Citations (83)

Reviews

Anti-H+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody) from Agrisera

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map