Anti-AOX1/2 antibody - Plant alternative oxidase 1 and 2 AS04 054 from Agrisera

Product Specs
ItemAnti-AOX1/2 antibody - Plant alternative oxidase 1 and 2
CompanyAgrisera
Price EUR329.00Supplier Page View Company Product Page
Catalog NumberAS04 054
ImmunogenKLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms including Arabidopsis thaliana AOX1A. UniProt: Q39219, TAIR: At3g22370, AOX1B UniProt: O23913, TAIR:AT3G22360, AOX1C UniProt: O22048, TAIR: AT3G27620, and AOX2, UniProt: O22049, TAIR: AT5G64210, Solanum lycopersicum UniProt: Q7XBG9, Oryza sativa UniProt: Q7XT33, AOX1D, TAIR: AT1G32350
Quantity50 µl
ApplicationsImmunolocalization, Western blot
ReactivityArabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Glycine max, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Rosa hybrida, Solanum lycopersicum, Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum, Zea mays
TargetAnti-AOX1/2
FormatLyophilized
ClonalityPolyclonal
HostRabbit
Research AreaPlant Biology
NCBI Gene AliasesATAOX1A, MSJ1_5, alternative oxidase 1A, hyper-sensitivity-related 3, HSR3, MSJ1.5, ALTERNATIVE OXIDASE, alternative oxidase 2, AtHSR3
PuritySerum
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Molecular Weight36-40 : 36-40 for Arabidopsis thaliana

Specifications/Features

1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection (WB), 1: 750 (IL)

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Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) Activation of alternative oxidase ensures carbon supply for ethylene and carotenoid biosynthesis during tomato fruit ripeningPlant PhysiologyOctober 31, 2025Ariadna Iglesias-Sanchez, Nestor Fernandez Del-Saz, Miguel Ezquerro, Elisenda Feixes-Prats, Miquel Ribas-Carbo, Alisdair R Fernie, Manuel Rodríguez-Concepción, Igor Florez-Sarasaelectrophoresis and transfer to nitrocellulose membrane, diluted 1:500 polyclonal anti-AOX AOX1 and 2 (AS04054, Agrisera, Sweden) or diluted 1:5000 anti-Porin, voltage-dependent anion-selective channel protein 1

(2) Targeted translation inhibition of chloroplast and mitochondrial mRNAs by designer pentatricopeptide repeat proteinsNucleic Acids ResMarch 26, 2025Nikolay Manavski, Serena Schwenkert, Hans-Henning Kunz, Dario Leister, Jörg Meurer(AS0-6172, 1:5000), PsaB (AS10-695, 1:1000), PetB (AS18-4169, 1:5000), Lhcb2 (AS01-003, 1:5000), AOX1/2 (AS04-054, 1:1000), Cox2 (AS04-053A, 1:1000), and AtpB (AS05-085, 1:5000) were obtained from Agrisera. PsbK (PHY4392A,

(3) Targeted introduction of premature stop codon in plant mitochondrial mRNA by a designer pentatricopeptide repeat protein with C-to-U editing functionPlant JFebruary 7, 2025Nikolay Manavski, Eslam Abdel-Salam, Serena Schwenkert, Hans-Henning Kunz, Andreas Brachmann, Dario Leister, Jörg MeurerScientific) and analyzed with a Fusion FX7 system (PeqLab, Erlangen, Germany). Antibodies targeting AOX1/2 (AS04 054; 1:1000), Cox2 (AS04 053A; 1:1,000), and AtpB (AS05 085; 1:5,000) were sourced from Agrisera (Vännäs,

(4) Mitochondrial AOX1a and an H2O2 feed‐forward signalling loop regulate flooding tolerance in ricePlant Biotechnology JournalNovember 12, 2024Cong Danh Nguyen, Chun‐Hsien Lu, Yi‐Shih Chen, Hsiang‐Ting Lee, Shuen‐Fang Lo, An‐Chi Wei, Tuan‐Hua David Ho, Su‐May Yuconducted as described previously (Chen et al., 2015). The anti‐plant alternative oxidase 1 and 2 (AOX) (AS04054) and anti‐α‐tubulin (AS10680) antibodies were purchased from Agrisera (Vännäs, Sweden). The anti‐AOX

(5) Germination of Pisum sativum L. Seeds Is Associated with the Alternative Respiratory PathwayBiology (Basel)October 9, 2023Lénia Rodrigues, Amaia Nogales, João Nunes, Leonardo Rodrigues, Lee D. Hansen, Hélia CardosoSweden, AS13 2640; dilution: 1:1000), and the lower part with primary antibody anti-AOX1/2 (Agrisera AS04054; dilution: 1:1000). PsActin was used as endogenous control for the normalization of data achieved for

(6) Poly(ADP-ribose)-binding protein RCD1 is a plant PARylation reader regulated by Photoregulatory Protein KinasesCommunications BiologyApril 19, 2023Julia P. Vainonen, Richard Gossens, Julia Krasensky-Wrzaczek, Raffaella De Masi, Iulia Danciu, Tuomas Puukko, Natalia Battchikova, Claudia Jonak, Lennart Wirthmueller, Michael Wrzaczek, et al.antibodies: αHA (Roche), αGFP (Milteny Biotech), αGST (Sigma), αPAR (Trevigen), αRCD15, and αAOX1/2 (Agrisera AS04 054). The signal was visualized by ECL Prime chemiluminescence reagents (GE Healthcare). Quantification

(7) Temperature dependence of O2 respiration in mangrove leaves and roots: implications for seedling dispersal phenologyNew PhytologistJanuary 1, 2023Tomomi Inoue, Yasuaki Akaji, Shigeyuki Baba, Ko Noguchi(AS04 053A; Agrisera, Vännäs, Sweden) and 250‐fold for use as polyclonal antibodies against the AOX protein (AS04 054; Agrisera). Horseradish peroxidase‐conjugated antirabbit IgG (DM 001A; Bio‐Techne) was used as a secondary

(8) Alternative or cytochrome? Respiratory pathways in traps of aquatic carnivorous bladderwort Utricularia reflexaPlant Signaling & BehaviorDecember 31, 2022Andrej PavloviÄ, Jana JakÅ¡ovÃ¡, Martin HrivÅˆackÃ½, LubomÃr Adamecphotosystem II (LHCB4) were performed as described previously10 with antibodies (cat. no. AS04 045, AS04 054, AS04 053A) purchased from Agrisera (Vännäs, Sweden). For measurements of RD, a Clark-type oxygen sensor

(9) Novel Insights into Floral Thermogenesis: In Vivo Analyses of Mitochondrial Dynamics in Nelumbo nucifera FlowersInternational Journal of Molecular SciencesOctober 8, 2022Ruoyi Li, Jing Li, Siqin Wang, Ruohan Wangdetermine the relative abundances of AOX and UCP, using a 1:2000 dilution of the anti-AOX1/2 antibody (AS04054; Agrisera AB, 21 Vännäs, Sweden) and 1:500 dilution of an anti-UCP1/2 antibody (AS121850; Agrisera AB,

(10) The Lack of Alternative Oxidase 1a Restricts in vivo Respiratory Activity and Stress-Related Metabolism for Leaf Osmoprotection and Redox Balancing Under Sudden Acute Water and Salt Stress in Arabidopsis thalianaFrontiers in Plant ScienceMay 17, 2022NÃ©stor F. Del-Saz, Ariadna Iglesias-Sanchez, David Alonso-Forn, Miguel LÃ³pez-GÃ³mez, Francisco Palma, MarÃa JosÃ© Clemente-Moreno, Alisdair R. Fernie, Miquel Ribas-Carbo, Igor Florez-SarasaBio-Rad. Membranes were incubated overnight at 4°C with diluted 1:500 polyclonal anti-AOX, AOX1 and 2 (AS04054, Agrisera, Sweden) or diluted 1:5,000 anti-Porin, voltage-dependent anion-selective channel protein

(11) Reductive stress triggers ANAC017-mediated retrograde signaling to safeguard the endoplasmic reticulum by boosting mitochondrial respiratory capacityThe Plant CellMarch 29, 2022Philippe Fuchs et al.in TBS-T (20 mM Tris pH 7.6 (HCl), 137 mM NaCl, 0.1% v/v Tween) overnight at 4°C. Antibodies AOX1/2 (AS04 054, Agrisera, Vännäs, Sweden) and goat anti-rabbit (AS09 602; Agrisera, Vännäs, Sweden) were diluted in

(12) ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction responsePlant PhysiologyAugust 3, 2021Jesús Pascual et al.AS09 521), α-YFP (antibodies-online ABIN1545635), α-SOT12 (Agrisera AS16 3943), or α-AOX (Agrisera AS04 054) antibodies, using horseradish peroxidase‐linked secondary antibody and enhanced chemiluminescence (Amersham,

(13) Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thalianaScientific ReportsDecember 1, 2020Kaori Sako et al.by non-reducing SDS-PAGE. Immunoblotting was performed as described previously43 using anti-AOX1/2 (AS04 054), anti-Gamma CA (AS17 4114) and anti-COX II (AS04 053A) (Agrisera, Vännäs, Sweden). Isolation of mitochondria.

(14) Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondriaBMC Plant BiologyDecember 1, 2020Magda Grabsztunowicz, Anne Rokka, Irum Farooq, Eva-Mari Aro, Paula Mulo[45], dilution 1:1000) was used as a root plastid marker, and alternative oxidase1/2 (AOX1/2, Agrisera, AS04054, dilution 1: 750) and nitrate reductase (NR; Agrisera, AS08 310–100, dilution: 1:500) antibodies were

(15) Mitochondrial Pentatricopeptide Repeat Protein, EMB2794, Plays a Pivotal Role in NADH Dehydrogenase Subunit nad2 mRNA Maturation in Arabidopsis thalianaPlant & cell physiologyJune 1, 2020Marchetti F, Cainzos M, Shevtsov S, Córdoba JP, Sultan LD, Brennicke A, Takenaka M, Pagnussat G, Ostersetzer-Biran O, Zabaleta E.

(16) Induction of Terminal Oxidases of Electron Transport Chain in Broccoli Heads under Controlled Atmosphere StorageFoodsMarch 25, 2020Yoshio Makino, Jun Inoue, Hsiao-Wen Wang, Masatoshi Yoshimura, Kensaku Maejima, Sachiko Funayama-Noguchi, Takeshi Yamada, Ko Noguchitransferred to a polyvinylidene difluoride membrane (Hybond-P, Amersham, Piscataway, NJ, USA). Anti-AOX (AS04 054, Agrisera, Vännäs, Sweden) and anti-COX II (AS04 053, Agrisera) were used as primary antibodies. Anti-Rabbit

(17) Altered levels of AOX1a expression result in changes in metabolic pathways in Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiationPlant science : an international journal of experimental plant biologyFebruary 1, 2020Garmash EV, Velegzhaninov IO, Ermolina KV, Rybak AV, Malyshev RV.

(18) The Lack of Mitochondrial Thioredoxin TRXo1 Affects In Vivo Alternative Oxidase Activity and Carbon Metabolism under Different Light ConditionsPlant and Cell PhysiologyNovember 1, 2019Igor Florez-Sarasa, Toshihiro Obata, NÃ¯Â¿Â½stor FernÃ¯Â¿Â½ndez Del-Saz, Jean-Philippe Reichheld, Etienne H Meyer, Manuel Rodriguez-Concepcion, Miquel Ribas-Carbo, Alisdair R FernieTom Elthon, Lincoln, NE) at 1:5000 dilution; polyclonal anti-AOX, 565 alternative oxidase 1 and 2 (AS04054, Agrisera, Sweden) at 1:500 dilution (used for the 566 total AOX amount); monoclonal anti-AOX (AOA,

(19) Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeksThe Plant journal : for cell and molecular biologyJuly 1, 2019Réthoré E, d'Andrea S, Benamar A, Cukier C, Tolleter D, Limami AM, Avelange-Macherel MH, Macherel D.

(20) Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport ProteinsFrontiers in Plant ScienceMay 15, 2019Liuqing Kuang, Shangwu Chen, Yan Guo, Huiqin MaAS08 325, 1:1000), AOX1/2 (plant alternative oxidase 1 and 2; mitochondrial marker, 36–40 kDa; Agrisera AS04 054, 1:1000). After three washes in PBST, the membranes were incubated in the secondary antibody (anti-rabbit

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Anti-AOX1/2 : Plant alternative oxidase 1 and 2

Anti-AOX1/2 antibody - Plant alternative oxidase 1 and 2 from Agrisera

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Specifications/Features

Agrisera

Citations (39)

Reviews

Anti-AOX1/2 antibody - Plant alternative oxidase 1 and 2 from Agrisera

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