Western blot was performed using Anti-EED Polyclonal Antibody (Product # PA5-34430) and a 45kDa band corresponding to human EED isoform and 56kDa band corresponding to mouse EED isoform was observed across cell lines and tissues tested. In addition to this there was an increase in the expression of EED proteins upon SAHA treatment in A549 cells. Whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with SAHA (2uM for 72hr) (Lane 2), tissue extracts (30 µg lysate) of Rat Kidney (Lane 3), Mouse Lung (Lane 4), Mouse Spleen (Lane 5) and Mouse Pancreas (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..